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BMC Immunology
Published in: BMC Immunology 1/2014

Open Access 01-12-2014 | Research article

Mesenchymal stromal cells reset the scatter factor system and cytokine network in experimental kidney transplantation

Authors: Marilena Gregorini, Francesca Bosio, Chiara Rocca, Valeria Corradetti, Teresa Valsania, Eleonora Francesca Pattonieri, Pasquale Esposito, Giulia Bedino, Chiara Collesi, Carmelo Libetta, Francesco Frassoni, Antonio Dal Canton, Teresa Rampino

Published in: BMC Immunology | Issue 1/2014

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Abstract

Background

In former studies we showed in a rat model of renal transplantation that Mesenchymal Stromal Cells (MSC) prevent acute rejection in an independent way of their endowing in the graft. In this study we investigated whether MSC operate by resetting cytokine network and Scatter Factor systems, i.e. Hepatocyte Growth Factor (HGF), Macrophage Stimulating Protein (MSP) and their receptors Met and RON, respectively.

Methods

MSC were injected into the renal artery soon after reperfusion. Controls were grafted untreated and normal rats. Rats were sacrificed 7 days after grafting. Serum and renal tissue levels of IFN-γ, IL-1, IL-2, IL-4, IL-6, IL-10, MSP/RON, HGF/Met systems, Treg lymphocytes were investigated.

Results

In grafted untreated rats IFN-γ increased in serum and renal tissue and IL-6 rose in serum. MSC prevented both the phenomena, increased IL-10 serum levels and Treg number in the graft. Furthermore MSC increased serum and tissue HGF levels, Met tubular expression and prevented the suppression of tubular MSP/RON expression.

Conclusions

Our results demonstrate that MSC modify cytokine network to a tolerogenic setting, they suppress Th1 cells, inactivate monocytes/macrophage, recruit Tregs. In addition, MSC sustain the expression of the Scatter Factor systems expression, i.e. systems that are committed to defend survival and stimulate regeneration of tubular cells.
Appendix
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Metadata
Title
Mesenchymal stromal cells reset the scatter factor system and cytokine network in experimental kidney transplantation
Authors
Marilena Gregorini
Francesca Bosio
Chiara Rocca
Valeria Corradetti
Teresa Valsania
Eleonora Francesca Pattonieri
Pasquale Esposito
Giulia Bedino
Chiara Collesi
Carmelo Libetta
Francesco Frassoni
Antonio Dal Canton
Teresa Rampino
Publication date
01-12-2014
Publisher
BioMed Central
Published in
BMC Immunology / Issue 1/2014
Electronic ISSN: 1471-2172
DOI
https://doi.org/10.1186/s12865-014-0044-1

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