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Critical Care
Published in: Critical Care 3/2011

Open Access 01-06-2011 | Research

Short term Candida albicans colonization reduces Pseudomonas aeruginosa-related lung injury and bacterial burden in a murine model

Authors: Florence Ader, Samir Jawhara, Saad Nseir, Eric Kipnis, Karine Faure, Fanny Vuotto, Chanez Chemani, Boualem Sendid, Daniel Poulain, Benoit Guery

Published in: Critical Care | Issue 3/2011

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Abstract

Introduction

Pseudomonas aeruginosa is a frequent cause of ventilator-acquired pneumonia (VAP). Candida tracheobronchial colonization is associated with higher rates of VAP related to P. aeruginosa. This study was designed to investigate whether prior short term Candida albicans airway colonization modulates the pathogenicity of P. aeruginosa in a murine model of pneumonia and to evaluate the effect of fungicidal drug caspofungin.

Methods

BALB/c mice received a single or a combined intratracheal administration of C. albicans (1 × 105 CFU/mouse) and P. aeruginosa (1 × 107 CFU/mouse) at time 0 (T0) upon C. albicans colonization, and Day 2. To evaluate the effect of antifungal therapy, mice received caspofungin intraperitoneally daily, either from T0 or from Day 1 post-colonization. After sacrifice at Day 4, lungs were analyzed for histological scoring, measurement of endothelial injury, and quantification of live P. aeruginosa and C. albicans. Blood samples were cultured for dissemination.

Results

A significant decrease in lung endothelial permeability, the amount of P. aeruginosa, and bronchiole inflammation was observed in case of prior C. albicans colonization. Mortality rate and bacterial dissemination were unchanged by prior C. albicans colonization. Caspofungin treatment from T0 (not from Day 1) increased their levels of endothelial permeability and lung P. aeruginosa load similarly to mice receiving P. aeruginosa alone.

Conclusions

P. aeruginosa-induced lung injury is reduced when preceded by short term C. albicans airway colonization. Antifungal drug caspofungin reverses that effect when used from T0 and not from Day 1.
Appendix
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Metadata
Title
Short term Candida albicans colonization reduces Pseudomonas aeruginosa-related lung injury and bacterial burden in a murine model
Authors
Florence Ader
Samir Jawhara
Saad Nseir
Eric Kipnis
Karine Faure
Fanny Vuotto
Chanez Chemani
Boualem Sendid
Daniel Poulain
Benoit Guery
Publication date
01-06-2011
Publisher
BioMed Central
Published in
Critical Care / Issue 3/2011
Electronic ISSN: 1364-8535
DOI
https://doi.org/10.1186/cc10276

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