Novel regulatory T cells controlling antibody production and systemic autoimmunity

Excerpt

Regulatory T cells (Tregs) are engaged in the maintenance of immunological self-tolerance and immune homeostasis. IL-10 has an important role in maintaining the normal immune state. We showed that IL-10-secreting Tregs can be delineated in normal mice as CD4⁺CD25^-Foxp3^- T cells that express lymphocyte activation gene-3 (LAG-3), an MHC class II-binding CD4 homolog. CD4⁺CD25^-LAG3⁺ Tregs characteristically express early growth response gene-2 (Egr-2), a key molecule for anergy induction. Retroviral gene transfer of Egr-2 converts naïve CD4⁺ T cells into IL-10-secreting and LAG-3-expressing Tregs. Moreover, CD4⁺CD25^-LAG3⁺ Tregs show B cell-dependent development. CD4⁺CD25^-LAG3⁺ Tregs, but not CD4⁺CD25⁺ Tregs, strongly suppressed the antibody production in B cells co-cultured with helper T cells. Thus, IL-10-secreting Egr-2⁺LAG3⁺CD4⁺ Tregs are closely related to B cells and can be exploited for the treat ment of autoimmune diseases. …