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Arthritis Research & Therapy
Published in: Arthritis Research & Therapy 1/2008

Open Access 01-02-2008 | Research article

Rutoside decreases human macrophage-derived inflammatory mediators and improves clinical signs in adjuvant-induced arthritis

Authors: Tina Kauss, Daniel Moynet, Jérôme Rambert, Abir Al-Kharrat, Stephane Brajot, Denis Thiolat, Rachid Ennemany, Fawaz Fawaz, M Djavad Mossalayi

Published in: Arthritis Research & Therapy | Issue 1/2008

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Abstract

Background

Dietary flavonols may play an important role in the adjunct therapy of chronic inflammation. The availability of therapeutic formulations of pentahydroxyflavone glycoside, rutoside (RU), led us to investigate the ability of this molecule to modulate the release of various proinflammatory mediators from human activated macrophages in vitro and to ameliorate arthritic markers in a rat model.

Methods

RU was added simultaneously to human macrophages during their activation. Cells were then analyzed for inflammation-related gene expression using a specific array, and cell supernatants were collected to measure inflammatory mediators. RU was also injected into adjuvant-induced arthritic rats, and disease progression and body weight were evaluated until 50 days after injection. Sera and peritoneal macrophages were also collected to quantify the RU effect on various inflammatory markers.

Results

RU inhibited inflammation-related gene expression in activated human macrophages and the release of nitric oxide, tumor necrosis factor-alpha, interleukin (IL)-1, and IL-6 from these cells. In a rat model, RU inhibited clinical signs of chronic arthritis, correlating with decreased levels of inflammatory cytokines detected in rat sera and macrophage supernatants.

Conclusion

Thus, RU may have clinical value in reducing inflammatory manifestations in human arthritis and other inflammatory diseases.
Appendix
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Metadata
Title
Rutoside decreases human macrophage-derived inflammatory mediators and improves clinical signs in adjuvant-induced arthritis
Authors
Tina Kauss
Daniel Moynet
Jérôme Rambert
Abir Al-Kharrat
Stephane Brajot
Denis Thiolat
Rachid Ennemany
Fawaz Fawaz
M Djavad Mossalayi
Publication date
01-02-2008
Publisher
BioMed Central
Published in
Arthritis Research & Therapy / Issue 1/2008
Electronic ISSN: 1478-6362
DOI
https://doi.org/10.1186/ar2372

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