Skip to main content
Key Specialties
Cardiology Diabetology Endocrinology Gastroenterology Geriatrics and Gerontology Gynecology and Obstetrics Hematology Infectious Disease Internal Medicine Nephrology Neurology Oncology Pediatrics Respiratory Medicine Rheumatology Surgery
Congress Coverage
2024 ACC Congress 2023 ESMO Congress 2023 EASD Congress 2023 ERS Congress 2023 ESC Congress 2023 EHA Congress 2023 ASCO Annual Meeting
Clinical Collections
Advances in Alzheimer’s

At a glance: The ONWARDS insulin icodec trials

A round-up of the ONWARDS phase 3 clinical trials evaluating the novel weekly insulin icodec in people with diabetes.
ADVANCED SEARCH
Log in
Top
European Journal of Medical Research
Published in: European Journal of Medical Research 1/2012

Open Access 01-12-2012 | Research

Protective effects of hyperbaric oxygen and iloprost on ischemia/reperfusion-induced lung injury in a rabbit model

Authors: Ş Bozok, G Ilhan, Y Yilmaz, Z Dökümcü, L Tumkaya, H Karamustafa, S Ozan Karakisi, Ş Ergene, E Şener

Published in: European Journal of Medical Research | Issue 1/2012

Login to get access

Abstract

Background

The role of multiorgan damage in the mortality caused by ischemic limb injury is still not clarified. The objective of this study was to examine the potential protective effects of hyperbaric oxygen (HBO) and iloprost (IL) therapy on lung damage induced by limb ischemia/reperfusion injury in a rabbit model, using both biochemical and histopathological aspects.

Methods

Forty New Zealand white rabbits were randomly allocated into one of five study groups: HBO group (single session of HBO treatment); IL group (25 ng/kg/min infusion of IL); HBO + IL group (both HBO and IL); Control group (0.9% saline only); and a sham group. Acute hind limb ischemia-reperfusion was established by clamping the abdominal aorta for 1 h. HBO treatment and IL infusion were administrated during 60 min of ischemia and 60 min of reperfusion period. Blood pH, partial pressure of oxygen, partial pressure of carbon dioxide and levels of bicarbonate, sodium, potassium, creatine kinase, lactate dehydrogenase, and tumor necrosis factor alpha were determined at the end of the reperfusion period. Malondialdehyde was measured in the plasma and lung as an indicator of free radicals. After sacrifice, left lungs were removed and histopathological examination determined the degree of lung injury.

Results

In the control group, blood partial pressure of oxygen and bicarbonate levels were significantly lower and creatine kinase, lactate dehydrogenase, malondialdehyde and tumor necrosis factor-α levels were significantly higher than those of the HBO group, IL group, HBO + IL group and sham group. Similarly, the malondialdehyde levels in the lung tissue and plasma levels were significantly lower in the treatment groups compared with the control group. The extent of lung injury according to the histological findings was significantly higher in the control group.

Conclusions

These results suggest that both HBO and IL therapies and their combination might be effectively used in the prevention of lung injury after ischemia/reperfusion injury of the lower extremities.
Appendix
Available only for authorised users
Literature
1.
Li C, Jackson RM: Reactive species mechanisms of cellular hypoxia-reoxygenation injury. Am J Physiol 2002, 282: C227-C241.CrossRef
2.
Groeneveld AB, Raijmakers PG, Rauwerda JA, Hack CE: The inflammatory response to vascular surgery-associated ischaemia and reperfusion in man: effect on postoperative pulmonary function. Eur J Vasc Endovasc Surg 1997, 14(5):351–359. 10.1016/S1078-5884(97)80284-5CrossRefPubMed
3.
Isbir S, Akgun S, Ak K, Civelek A, Tekeli A, Cobanoglu A: Effect of acute lower limb ischemia/reperfusion injury on the free oxygen radical systems in lung. Turkish J Cardiovasc Surg 2000, 8(2):632–634.
4.
Valenzuela A: The biological significance of malondialdehyde determination in the assessment of tissue oxidative stress. Life Sci 1991, 48: 301–309. 10.1016/0024-3205(91)90550-UCrossRefPubMed
5.
Wijnen MH, Roumen RM, Vader HL, Goris RJ: A multiantioxidant supplementation reduces damage from ischaemia reperfusion in patients after lower torso ischaemia. A randomised trial. Eur J Vasc Endovasc Surg 2002, 23(6):486–490. 10.1053/ejvs.2002.1614CrossRefPubMed
6.
Novelli GP, Adembri C, Gandini E, Orlandini SZ, Papucci L, Formigli L, Manneschi LI, Quattrone A, Pratesi C, Capaccioli S: Vitamin E protects human skeletal muscle from damage during surgical ischemia-reperfusion. Am J Surg 1997, 173(3):206–209. 10.1016/S0002-9610(97)89593-1CrossRefPubMed
7.
Buras J: Basic mechanisms of hyperbaric oxygen in the treatment of ischemia reperfusion injury. Int Anesthesiol Clin 2000, 38(1):91–109. 10.1097/00004311-200001000-00007CrossRefPubMed
8.
Tjärnström J, Wikström T, Bagge U, Risberg B, Braide M: Effects of hyperbaric oxygen treatment on neutrophil activation and pulmonary sequestration intestinal ischemia-reperfusion in rats. Eur Surg Res 1999, 31(2):147–154. 10.1159/000008633CrossRefPubMed
9.
Tibbles PM, Edelsberg JS: Hyperbaric oxygen therapy. N Engl J Med 1996, 334: 1642–1648. 10.1056/NEJM199606203342506CrossRefPubMed
10.
Fantone JC, Marasco WA, Elgas LJ, Ward PA: Stimulus specificity of prostaglandin inhibition of rabbit polymorphonuclear leukocyte lysosomal enzyme release and superoxide anion production. Am J Pathol 1984, 115(1):9–16.PubMedCentralPubMed
11.
Baltalarli A, Ozcan V, Bir F, Aybek H, Sacar M, Onem G, Goksin I, Demir S, Teke Z: Ascorbic acid (vitamin C) and iloprost attenuate the lung injury caused by ischemia/reperfusion of the lower extremities of rats. Ann Vasc Surg 2006, 20(1):49–55. 10.1007/s10016-005-9284-0CrossRefPubMed
12.
Ashikaga H, Leclercq C, Wang J, Kass DA, McVeigh ER: Hemodynamic improvement in cardiac resynchronization does not require improvement in left ventricular rotation mechanics: three-dimensional tagged MRI analysis. Circ Cardiovasc Imaging 2010 Jul, 3(4):456–63. Epub 2010 May 17 10.1161/CIRCIMAGING.109.906305PubMedCentralCrossRefPubMed
13.
Pizarro TT, Malinowska K, Kovacf EJ, Clancy L, Robinson JA, Piccinini LA: Induction of TNF and TNF/ß (lymphotoxin) gene expression in rat cardiac transplants during allograft rejection. Transplantation 1993, 56: 399–404. 10.1097/00007890-199308000-00029CrossRefPubMed
14.
Uchiyama M, Mihara M: Determination of malonaldehyde precursor in tissues by thiobarbituric acid test. Anal Biochem 1978, 86(1):271–278. 10.1016/0003-2697(78)90342-1CrossRefPubMed
15.
Deeb GM, Grum CM, Lynch MJ, Guynn TP, Gallagher KP, Ljungman AG, Bolling SF, Morganroth ML: Neutrophils are not necessary for induction of ischemia-reperfusion lung injury. J Appl Physiol 1990, 68(1):374–381. 10.1063/1.347149CrossRefPubMed
16.
Welbourn R, Goldman G, Kobzik L, Paterson IS, Valeri CR, Shepro D, Hechtman HB: Role of neutrophil adherence receptors (CD 18) in lung permeability following lower torso ischemia. Circ Res 1992, 71(1):82–86. 10.1161/01.RES.71.1.82CrossRefPubMed
17.
Sirin H, Saribulbul O, Cerrahoglu M, Aksoy O, Baltalarli A, Sacar M: [Protective effect of aprotinin on lung injury caused by ischemia-reperfusion of the lower extremities]. Turkish J Cardiovasc Surg 2001, 9(4):233–237.
18.
Yasa H, Yakut N, Emrecan B, Ergunes K, Ortac R, Karahan N, Ozbek C, Gurbuz A: Protective effects of levosimendan and iloprost on lung injury induced by limb ischemia-reperfusion: a rabbit model. J Surg Res 2008, 147(1):138–142. 10.1016/j.jss.2007.08.002CrossRefPubMed
19.
Klausner JM, Anner H, Paterson IS, Kobzik L, Valeri CR, Shepro D, Hechtman HB: Lower torso ischaemia-induced lung injury is leukocyte dependent. Ann Surg 1988, 208(6):761–767. 10.1097/00000658-198812000-00015PubMedCentralCrossRefPubMed
20.
Hung TK, Pai MP: The effect of varying ambient oxygen tensions on wound metabolism and collagen synthesis. Surg Gynecol Obstet 1972, 135: 561–567.
21.
Hunt TK, Zederfelt BH, Goldstick TK: Oxygen and healing. Am J Surg 1969, 118: 521–525. 10.1016/0002-9610(69)90174-3CrossRefPubMed
22.
Zamboni WA, Roth AC, Russell RC: Morphological analysis of microcirculation during reperfusion of ischemic skeletal muscle and the effect of hyperbaric oxygen. Plast Reconstr Surg 1993, 91: 1110–1123. 10.1097/00006534-199305000-00022CrossRefPubMed
23.
Thom SR: Functional inhibition of leukocyte B2 integrins by hyperbaric oxygen in carbon monoxide-mediated brain injury in rats. Toxicol Appl Pharmacol 1993, 123: 248–256. 10.1006/taap.1993.1243CrossRefPubMed
24.
Rossman JE, Caty MG, Zheng S, Karamanoukian HL, Thusu K, Azizkhan RG, Dandona P: Mucosal protection from intestinal ischemia-reperfusion reduces oxidant injury to the lung. J Surg Res 1997, 73(1):41–46. 10.1006/jsre.1997.5196CrossRefPubMed
25.
Ueno S, Tanabe G, Kihara K, Aoki D, Arikawa K, Dogomori H, Aikou T: Early post-operative hyperbaric oxygen therapy modifies neutrophil activation. Hepatogastroenterology 1999, 46(27):1798–1799.PubMed
26.
Haapaniemi T, Nylander G, Sirsjo A, Larsson J: Hyperbaric oxygen reduces ischemia-induced skeletal muscle injury. Plast Reconstr Surg 1996, 97(3):602–607. 10.1097/00006534-199603000-00017CrossRefPubMed
27.
Chen MF, Chen HM, Ueng SW, Shyr MH: Hyperbaric oxygen pretreatment attenuates hepatic reperfusion injury. Liver 1998, 18(2):110–116.CrossRefPubMed
28.
Kudchodkar BJ, Wilson J, Lacko A, Dory L: Hyperbaric oxygen reduces the progression and accelerates the regression of atherosclerosis in rabbits. Arterioscler Thromb Vasc Biol 2000, 20(6):1637–1643. 10.1161/01.ATV.20.6.1637CrossRefPubMed
29.
Belkin M, Wright JG, Hobson RW: Iloprost infusion decreases skeletal muscle ischemia-reperfusion injury. J Vasc Surg 1990, 11(1):77–83.CrossRefPubMed
30.
Katircioglu SF, Ulus AT, Gokce P, Surucu S: Iloprost protects the spinal cord during aortic cross-clamping in a canine model. J Cardiovasc Surg (Torino) 2000, 1: 89–93.
31.
Coskun K, Attar A, Tuna H, Sargon MF, Yuceer N, Turker RK, Egemen N: Early protective effects of iloprost after experimental spinal cord ischemia in rabbits. Acta Neurochir (Wien) 2000, 10: 1143–1150.CrossRef
32.
33.
Turnage RH, Guice KS, Oldham KT: Pulmonary micro-vascular injury following intestinal reperfusion. New Horiz 1994, 2: 463–475.PubMed
34.
Caty MG, Guice KS, Oldham KT, Remick DG, Kunkel SL: Evidence for tumor necrosis factor-induced pulmonary micro- vascular injury after intestinal ischemia-reperfusion injury. Ann Surg 1989, 212: 694–700.CrossRef
35.
Sorkine P, Setton A, Miller A, Rudick V, Marmor S, Klausner JM, Goldman G: Soluble tumor necrosis factor receptors reduce bowel ischemia-induced lung permeability and neutrophil sequestration. Crit Care Med 1995, 23: 1377–1381. 10.1097/00003246-199508000-00011CrossRefPubMed
36.
Yang ZJ, Bosco G, Montante A, Ou XI, Camporesi EM: Hyperbaric O2 reduces intestinal ischemia-reperfusion-induced TNF-alpha production and lung neutrophil sequestration. Eur J Appl Physiol 2001, 85(1–2):96–103.CrossRefPubMed
37.
Massimo G, Milesi S, Donato S, Raffaelli M: The hemodynamic and metabolic effects of tourniquet application during knee surgery. Anesth Analg 2000, 91: 727–731. 10.1213/00000539-200009000-00043CrossRef
Metadata
Title
Protective effects of hyperbaric oxygen and iloprost on ischemia/reperfusion-induced lung injury in a rabbit model
Authors
Ş Bozok
G Ilhan
Y Yilmaz
Z Dökümcü
L Tumkaya
H Karamustafa
S Ozan Karakisi
Ş Ergene
E Şener
Publication date
01-12-2012
Publisher
BioMed Central
Published in
European Journal of Medical Research / Issue 1/2012
Electronic ISSN: 2047-783X
DOI
https://doi.org/10.1186/2047-783X-17-14

Other articles of this Issue 1/2012

European Journal of Medical Research 1/2012 Go to the issue

Research

Management of EGFR-inhibitor associated rash: a retrospective study in 49 patients

Research

Systematic assessment of microneedle injection into the mouse cornea

Research

Abdominal obesity and prolonged prone positioning increase risk of developing sclerosing cholangitis in critically ill patients with influenza A-associated ARDS

Research

Effects and mechanism of duodenal-jejunal bypass and sleeve gastrectomy on GLUT2 and glucokinase in diabetic Goto–Kakizaki rats

Research

Tumor growth effects of rapamycin on human biliary tract cancer cells

Research

Common polymorphisms in GSTM1, GSTT1, GSTP1, GSTA1 and susceptibility to colorectal cancer in the Central European population