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Open Access 01-12-2012 | Research

Intact skin and not stripped skin is crucial for the safety and efficacy of peanut epicutaneous immunotherapy (EPIT) in mice

Authors: Lucie Mondoulet, Vincent Dioszeghy, Emilie Puteaux, Mélanie Ligouis, Véronique Dhelft, Franck Letourneur, Christophe Dupont, Pierre-Henri Benhamou

Published in: Clinical and Translational Allergy | Issue 1/2012

Abstract

Background

Epicutaneous immunotherapy (EPIT) on intact skin with an epicutaneous delivery system has already been used in preclinical and clinical studies. In epicutaneous vaccination and immunotherapy, the stripping of skin before application of the allergen is suggested to facilitate the passage of allergen through immune cells.

Objectives

The aim of this study was to compare the immunological response induced by EPIT performed on intact and stripped skin in a mouse model of peanut allergy.

Methods

After oral sensitization with peanut and cholera toxin, BALB/c mice were epicutaneously treated using an epicutaneous delivery system (Viaskin® (DBV Technologies, Paris) applied either on intact skin or on stripped skin. Following EPIT, mice received an exclusive oral peanut regimen, aimed at triggering esophageal and jejunal lesions. We assessed eosinophil infiltration by histology, mRNA expression in the esophagus, antibody levels and peripheral T-cell response.

Results

EPIT on intact skin significantly reduced Th2 immunological response (IgE response and splenocyte secretion of Th2 cytokines) as well as esophageal eosinophilia (2.7 ± 0.9, compared to Sham 19.9 ± 1.5, p < 0.01), mRNA expression of Th2 cytokines in tissue and intestinal villus sub-atrophia (2.9 ± 0.2 vs Sham, 2.1 ± 0.2, p < 0.05). By contrast, EPIT on stripped skin reinforced Th2 systemic immunological response as well as eosinophil infiltration (26.8 ± 15.1), mRNA expression of Th2 cytokines and duodenal villus/crypt-ratio (2.4 ± 0.3).

Conclusions

Epicutaneous allergen-specific immunotherapy needs the integrity of superficial layers of the stratum corneum to warranty safety of treatment and to induce a tolerogenic profile of the immune response.

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Title: Intact skin and not stripped skin is crucial for the safety and efficacy of peanut epicutaneous immunotherapy (EPIT) in mice
Authors: Lucie Mondoulet
Vincent Dioszeghy
Emilie Puteaux
Mélanie Ligouis
Véronique Dhelft
Franck Letourneur
Christophe Dupont
Pierre-Henri Benhamou
Publication date: 01-12-2012
Publisher: BioMed Central
Published in: Clinical and Translational Allergy / Issue 1/2012
Electronic ISSN: 2045-7022
DOI: https://doi.org/10.1186/2045-7022-2-22

Keynote webinar | Spotlight on sleep in brain health

Springer Medicine

Intact skin and not stripped skin is crucial for the safety and efficacy of peanut epicutaneous immunotherapy (EPIT) in mice

Abstract

Background

Objectives

Methods

Results

Conclusions

Keynote webinar | Spotlight on sleep in brain health

Springer Medicine

Abstract

Background

Objectives

Methods

Results

Conclusions

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