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Herpesviridae
Published in: Herpesviridae 1/2014

Open Access 01-12-2014 | Research

Epstein-Barr virus IL-10 gene expression by a recombinant murine gammaherpesvirus in vivoenhances acute pathogenicity but does not affect latency or reactivation

Authors: Gary J Lindquester, Kimberly A Greer, James P Stewart, Jeffery T Sample

Published in: Herpesviridae | Issue 1/2014

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Abstract

Background

Many viral genes affect cytokine function within infected hosts, with interleukin 10 (IL-10) as a commonly targeted mediator. Epstein-Barr virus (EBV) encodes an IL-10 homologue (vIL-10) expressed during productive (lytic) infection and induces expression of cellular IL-10 (cIL-10) during latency. This study explored the role of vIL-10 in a murine gammaherpesvirus (MHV) model of viral infection.

Methods

The EBV vIL-10 gene was inserted into MHV-76, a strain which lacks the ability to induce cIL-10, by recombination in transfected mouse cells. Mice were infected intranasally with the recombinant, vIL-10-containing MHV-76 or control virus strains and assayed at various days post infection for lung virus titer, spleen cell number, percentage of latently infected spleen cells and ability to reactivate virus from spleen cells.

Results

Recombinant murine gammaherpesvirus expressing EBV vIL-10 rose to significantly higher titers in lungs and promoted an increase in spleen cell number in infected mice in comparison to MHV strains lacking the vIL-10 gene. However, vIL-10 expression did not alter the quantity of latent virus in the spleen or its ability to reactivate.

Conclusions

In this mouse model of gammaherpesvirus infection, EBV vIL-10 appears to influence acute-phase pathogenicity. Given that EBV and MHV wild-type strains contain other genes that induce cIL-10 expression in latency (e.g. LMP-1 and M2, respectively), vIL-10 may have evolved to serve the specific role in acute infection of enlarging the permissive host cell population, perhaps to facilitate initial survival and dissemination of viral-infected cells.
Appendix
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Metadata
Title
Epstein-Barr virus IL-10 gene expression by a recombinant murine gammaherpesvirus in vivoenhances acute pathogenicity but does not affect latency or reactivation
Authors
Gary J Lindquester
Kimberly A Greer
James P Stewart
Jeffery T Sample
Publication date
01-12-2014
Publisher
BioMed Central
Published in
Herpesviridae / Issue 1/2014
Electronic ISSN: 2042-4280
DOI
https://doi.org/10.1186/2042-4280-5-1

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