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Experimental & Translational Stroke Medicine

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Open Access 01-12-2014 | Short report

Intravenous immunoglobulin (IVIg) provides protection against endothelial cell dysfunction and death in ischemic stroke

Authors: Alexander Widiapradja, Tomislav Santro, Milan Basta, Christopher G Sobey, Silvia Manzanero, Thiruma V Arumugam

Published in: Experimental & Translational Stroke Medicine | Issue 1/2014

Abstract

Background

The brain endothelium is a key component of the blood brain barrier which is compromised following ischemia, allowing infiltration of damaging immune cells and other inflammatory molecules into the brain. Intravenous immunoglobulin (IVIg) is known to reduce infarct size in a mouse model of experimental stroke.

Findings

Flow cytometry analysis showed that the protective effect of IVIg in ischemia and reperfusion injury in vivo is associated with reduced leukocyte infiltration, suggesting an involvement of the endothelium. In an in vitro model of ischemia, permeability analysis of the mouse brain endothelial cell line bEnd.3 revealed that IVIg prevented the loss of permeability caused by oxygen and glucose deprivation (OGD). In addition, western blot analysis of these brain endothelial cells showed that IVIg prevented the down-regulation of tight junction proteins claudin 5 and occludin and the decline in anti-apoptotic proteins Bcl-2 and Bcl-XL caused by OGD.

Conclusion

IVIg protects endothelial cells from ischemic insult. These studies support the use of IVIg as a pharmacological intervention for stroke therapy.

Available only for authorised users

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Title: Intravenous immunoglobulin (IVIg) provides protection against endothelial cell dysfunction and death in ischemic stroke
Authors: Alexander Widiapradja
Tomislav Santro
Milan Basta
Christopher G Sobey
Silvia Manzanero
Thiruma V Arumugam
Publication date: 01-12-2014
Publisher: BioMed Central
Published in: Experimental & Translational Stroke Medicine / Issue 1/2014
Electronic ISSN: 2040-7378
DOI: https://doi.org/10.1186/2040-7378-6-7

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Springer Medicine

Intravenous immunoglobulin (IVIg) provides protection against endothelial cell dysfunction and death in ischemic stroke

Abstract

Background

Findings

Conclusion

Keynote webinar | Spotlight on sleep in brain health

Springer Medicine

Abstract

Background

Findings

Conclusion

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