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Open Access 01-12-2014 | Commentary

Proper paraffin slide storage is crucial for translational research projects involving immunohistochemistry stains

Authors: Mary Economou, Liliane Schöni, Caroline Hammer, José A Galván, Dominique-Elisabeth Mueller, Inti Zlobec

Published in: Clinical and Translational Medicine | Issue 1/2014

Abstract

The use of paraffin slides and tissue microarrays (TMA) is indispensable for translational research. However, storage of paraffin slides over time has a substantial detrimental effect on the quality and reliability of immunohistochemistry stains. Particularly affected by this issue may be any collaborative efforts where paraffin slides or TMAs are shipped to central laboratories and then ‘biobanked’ for some time until use. This article summarizes some of the key issues affecting loss of antigenicity on paraffin slides and some simple storage solutions to help maintain high quality immunohistochemistry results when paraffin slides must be stored for a certain time prior to use.

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Title: Proper paraffin slide storage is crucial for translational research projects involving immunohistochemistry stains
Authors: Mary Economou
Liliane Schöni
Caroline Hammer
José A Galván
Dominique-Elisabeth Mueller
Inti Zlobec
Publication date: 01-12-2014
Publisher: Springer Berlin Heidelberg
Published in: Clinical and Translational Medicine / Issue 1/2014
Electronic ISSN: 2001-1326
DOI: https://doi.org/10.1186/2001-1326-3-4

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Proper paraffin slide storage is crucial for translational research projects involving immunohistochemistry stains

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