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Open Access 01-12-2015 | Research

Oxidative DNA damage in diabetic and mild gestational hyperglycemic pregnant women

Authors: Rafael Bottaro Gelaleti, Débora Cristina Damasceno, Paula Helena Ortiz Lima, Daisy Maria Favero Salvadori, Iracema de Mattos Paranhos Calderon, José Carlos Peraçoli, Marilza Vieira Cunha Rudge

Published in: Diabetology & Metabolic Syndrome | Issue 1/2015

Abstract

Background

Pregnant women with mild gestational hyperglycemia present high risk for hypertension, obesity and hyperglycemia, and appeared to reproduce the model of metabolic syndrome in pregnancy, with hyperinsulinemia and insulin resistance. Our clinical studies showed that mild gestational hyperglycemia or gestational diabetes are related to similar adverse maternal and perinatal outcomes. Hyperglycemia and other factors associated with diabetes generate reactive oxygen species that increase DNA damage levels. The aim of this study was to evaluate oxidative DNA damage in lymphocytes of pregnant women with diabetes or mild gestational hyperglycemia.

Methods

The study included 111 pregnant women distributed into three groups based on oral glucose tolerance test (OGTT) and glycemic profiles (GP), as follows: Normal OGTT and GP (control group); Normal OGTT and abnormal GP (mild gestational hyperglycemia group); Abnormal OGTT and GP (diabetic group). Maternal blood samples (5–10 mL) were collected and processed for determination of oxidative DNA damage by the comet assay, using Fpg and Endo III enzymes. Urine samples were also collected for determination of 8-OHdG concentrations by ELISA.

Results

Subjects in the diabetes group presented increased amount of oxidized purines, while mild gestational hyperglycemia women presented with increased oxidized pyrimidines, compared to the control group.

Conclusion

Gestational, overt diabetes and mild gestational hyperglycemia, were all related to increased oxidative DNA damage. Diabetic pregnant women showed increased level of oxidative DNA damage, perhaps mainly due to hyperglycemia. On the other hand, oxidative DNA damage detected in women with mild gestational hyperglycemia might be associated with repercussions from obesity, hypertension and/or insulin resistance. Interestingly, the type of DNA base affected seemed to be dependent on the glycemic profile or oxidative stress.

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Title: Oxidative DNA damage in diabetic and mild gestational hyperglycemic pregnant women
Authors: Rafael Bottaro Gelaleti
Débora Cristina Damasceno
Paula Helena Ortiz Lima
Daisy Maria Favero Salvadori
Iracema de Mattos Paranhos Calderon
José Carlos Peraçoli
Marilza Vieira Cunha Rudge
Publication date: 01-12-2015
Publisher: BioMed Central
Published in: Diabetology & Metabolic Syndrome / Issue 1/2015
Electronic ISSN: 1758-5996
DOI: https://doi.org/10.1186/1758-5996-7-1

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