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Journal of Experimental & Clinical Cancer Research
Published in: Journal of Experimental & Clinical Cancer Research 1/2009

Open Access 01-12-2009 | Research

MMP7 expression regulated by endocrine therapy in ERβ-positive colon cancer cells

Authors: Yu-Jing Fang, Zhi-Zhong Pan, Li-Ren Li, Zhen-Hai Lu, Li-Yi Zhang, De-Sen Wan

Published in: Journal of Experimental & Clinical Cancer Research | Issue 1/2009

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Abstract

Background

Many studies have shown that colon cancer is an estrogen-dependent carcinoma. This study explored the efficacy of endocrine therapy in colon cancer cells with high metastatic potential (HT29). We investigated the proliferation of HT29 cells after exposure to endocrine therapy (tamoxifen) and 5-FU.

Methods

Apoptosis was evaluated using flow cytometry. The expression of matrix metalloproteinases 7 (MMP-7) and estrogen receptor beta (ERβ) was measured by reverse transcription-polymerase chain reaction (RT-PCR) and western blot. The migration capability of treated cells was determined with wound scratch assay.

Results

Tamoxifen alone, 5-FU alone, and the combination of the two drugs can significantly inhibit HT29 cell proliferation and migration, block the cells in G2/M phase and induce cell apoptosis. These drugs also can down-regulate MMP7 and ERβ expression.

Conclusion

Our findings suggest that endocrine therapy is an efficient therapy for inhibiting ERβ-positive colon cancer cell proliferation and migration via down-regulation of MMP7.
Appendix
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Metadata
Title
MMP7 expression regulated by endocrine therapy in ERβ-positive colon cancer cells
Authors
Yu-Jing Fang
Zhi-Zhong Pan
Li-Ren Li
Zhen-Hai Lu
Li-Yi Zhang
De-Sen Wan
Publication date
01-12-2009
Publisher
BioMed Central
Published in
Journal of Experimental & Clinical Cancer Research / Issue 1/2009
Electronic ISSN: 1756-9966
DOI
https://doi.org/10.1186/1756-9966-28-132

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