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Thyroid Research

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Open Access 01-12-2009 | Research

Endogenous avidin biotin activity (EABA) in thyroid pathology: immunohistochemical study

Authors: Barbara Nikiel, Mykola Chekan, Michal Jarząb, Dariusz Lange

Published in: Thyroid Research | Issue 1/2009

Abstract

Background

Immunohistochemical methods based on the high affinity of avidin and biotin (e.g. ABC, LSAB) are characterized by high sensitivity and are widely used for detection of immunologic reaction. However, a non-specific reaction, observed in frozen tissues and in paraffin-embedded material, increasing after heat induced epitope retrieval (HIER), and caused either by endogenous biotin or any another chemical compound with high affinity for avidin, may lead to diagnostic mistakes. The aim of our investigation is to study presence of endogenous avidin biotin activity (EABA) in thyrocytes originating from various thyroid pathological lesions (neoplastic and non-neoplastic).

Materials and methods

The immunohistochemical study was performed on paraffin-embedded specimens of surgically resected thyroid tissue from 97 patients with thyroid diseases: 65 patients with papillary carcinoma (PTC), 11 patients with nodular goiter in whom features of benign papillary hyperplasia were found, 9 with lymphocytic thyroiditis (LT), 8 with follicular adenoma, and 4 patients with follicular carcinoma. In PTC immunohistochemical study was performed both in primary tumors and in lymph node metastases. After HIER, incubation with streptavidin from LSAB+ (DakoCytomation) kit was done.

Results

Strong cytoplasmic EABA was observed in 56 of 65 (87.5%) PTC and in oxyphilic cells in 8 of 9 cases of LT. Significant correlation between EABA in primary PTC tumor and EABA in lymph node metastases was stated. Normal surrounding thyroid tissues showed absence or weak EABA. Aberrant intranuclear localization of biotin was noted in morules of cribriform-morular variant of PTC. No statistically significant correlation between patient's age, sex, metastases presence and EABA was observed.

Conclusion

Among thyroid lesions, false positive reactions are highly probable in papillary thyroid carcinoma and in lymphocytic thyroiditis if immunohistochemical detection is used on systems containing (strept)avidin. The most probable reason is the high endogenous biotin content.

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Garrett RH, Grisham CM: Biochemistry. 2nd edition. Philadelphia, Pennsylvania: Saunders College Publishing; 1999.

Dakshinamurti K: Biotin – a regulator of gene expression. Journal of Nutritional Biochemistry 2005, 419–423. 10.1016/j.jnutbio.2005.03.015

Leon-Del-Rio A: Biotin – dependent regulation of gene expression in human cells. Journal of Nutritional Biochemistry 2005, 432–434. 10.1016/j.jnutbio.2005.03.021

Zhou X, Wang P, Lu M, Liu L, Zhang Y, Zhang S, Chen G, Zhang C, Huang S: The influence of heat-induced epitope retrieval on endogenous avidin-binding activity (EABA) and blocking of EABA in immunohistochemistry. Zhonghua Bing Li Xue Za Zhi 2002, 31: 491–496.PubMed

Banerjee D, Pettit S: Endogenous avidin-binding activity in human lymphoid tissue. J Clin Pathol 1984, 37: 223–225. 10.1136/jcp.37.2.223PubMedCentralPubMedCrossRef

Kashima K, Yokoyama S, Daa T, Nakayama I, Nickerson PA, Noguchi S: Cytoplasmic biotin-like activity interferes with immunohistochemical analysis of thyroid lesions: a comparison of antigen retrieval methods. Mod Pathol 1997, 10: 515–519.PubMed

Kern DA, Smith MM, Grant JW, Rockhill AD: Evaluation of bending strength of five interdental fixation apparatuses applied to canine mandibles. Am J Vet Res 1993, 54: 1177–1182.PubMed

Matsumoto Y: Simultaneous inhibition of endogenous avidin-binding activity and peroxidase applicable for the avidin-biotin system using monoclonal antibodies. Histochemistry 1985, 83: 325–330. 10.1007/BF00684378PubMedCrossRef

Bussolati G, Gugliotta P, Volante M, Pace M, Papotti M: Retrieved endogenous biotin: a novel marker and a potential pitfall in diagnostic immunohistochemistry. Histopathology 1997, 31: 400–407. 10.1046/j.1365-2559.1997.3020895.xPubMedCrossRef

10.

Mount SL, Cooper K: Beware of biotin: a source of false-positive immunohistochemistry. Current Diagnostic Pathology 2001, 161–167. 10.1054/cdip.2001.0069

11.

Kim SH, Jung KC, Shin YK, Lee KM, Park YS, Choi YL, Oh KI, Kim MK, Chung DH, Son HG, Park SH: The enhanced reactivity of endogenous biotin-like molecules by antigen retrieval procedures and signal amplification with tyramine. Histochem J 2002, 34: 97–103. 10.1023/A:1020954611464PubMedCrossRef

12.

Matsumoto F, Fujii H, Abe M, Kajino K, Kobayashi T, Matsumoto T, Ikeda K, Hino O: A novel tumor marker, Niban, is expressed in subsets of thyroid tumors and Hashimoto's thyroiditis. Hum Pathol 2006, 37: 1592–1600. 10.1016/j.humpath.2006.06.022PubMedCrossRef

13.

Herrmann ME, LiVolsi VA, Pasha TL, Roberts SA, Wojcik EM, Baloch ZW: Immunohistochemical expression of galectin-3 in benign and malignant thyroid lesions. Arch Pathol Lab Med 2002, 126: 710–713.PubMed

14.

Pacheco-Alvarez D, Solorzano-Vargas RS, Del Rio AL: Biotin in metabolism and its relationship to human disease. Arch Med Res 2002, 33: 439–447. 10.1016/S0188-4409(02)00399-5PubMedCrossRef

15.

Hymes J, Wolf B: Biotinidase and its roles in biotin metabolism. Clin Chim Acta 1996, 255: 1–11. 10.1016/0009-8981(96)06396-6PubMedCrossRef

16.

Wolf B: Biotinidase: its role in biotinidase deficiency and biotin metabolism. J Nutr Biochem 2005, 16: 441–445. 10.1016/j.jnutbio.2005.03.024PubMedCrossRef

17.

Hymes J, Wolf B: Biotinidase and its role in biotin metabolism. Clinica Chimica Acta 1996, 255: 1–11. 10.1016/0009-8981(96)06396-6CrossRef

18.

Wolf B: Biotinidase: its role in biotinidase deficiency and biotin metabolism. Journal of Nutritional Biochemistry 2005, 16: 441–445. 10.1016/j.jnutbio.2005.03.024PubMedCrossRef

19.

Rodriguez-Melendez R, Cano S, Mendez ST, Velazquez A: Biotin regulates the genetic expression of holocarboxylase synthetase and mitochondrial carboxylases in rats. J Nutr 2001, 131: 1909–1913.PubMed

20.

Leon-Del-Rio A: Biotin-dependent regulation of gene expression in human cells. J Nutr Biochem 2005, 16: 432–434. 10.1016/j.jnutbio.2005.03.021PubMedCrossRef

21.

Praul CA, Brubaker KD, Leach RM, Gay CV: Detection of endogenous biotin-containing proteins in bone and cartilage cells with streptavidin systems. Biochem Biophys Res Commun 1998, 247: 312–314. 10.1006/bbrc.1998.8757PubMedCrossRef

22.

Wood GS, Warnke R: Suppression of endogenous avidin-binding activity in tissues and its relevance to biotin-avidin detection systems. J Histochem Cytochem 1981, 29: 1196–1204.PubMedCrossRef

23.

Arif S, Blanes A, Diaz-Cano SJ: Hashimoto thyroiditis shares features with early papillary thyroid carcinoma. Histopatology 2002, 357–362. 10.1046/j.1365-2559.2002.01467.x

Title: Endogenous avidin biotin activity (EABA) in thyroid pathology: immunohistochemical study
Authors: Barbara Nikiel
Mykola Chekan
Michal Jarząb
Dariusz Lange
Publication date: 01-12-2009
Publisher: BioMed Central
Published in: Thyroid Research / Issue 1/2009
Electronic ISSN: 1756-6614
DOI: https://doi.org/10.1186/1756-6614-2-5

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Conclusion

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