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Radiation Oncology
Published in: Radiation Oncology 1/2010

Open Access 01-12-2010 | Research

Early administration of IL-6RA does not prevent radiation-induced lung injury in mice

Authors: Toshiyuki Ogata, Hideya Yamazaki, Teruki Teshima, Ayaka Kihara, Yuko Suzumoto, Takehiro Inoue, Norihiro Nishimoto, Nariaki Matsuura

Published in: Radiation Oncology | Issue 1/2010

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Abstract

Background

Radiation pneumonia and subsequent radiation lung fibrosis are major dose-limiting complications for patients undergoing thoracic radiotherapy. Interleukin-6 (IL-6) is a pleiotropic cytokine and plays important roles in the regulation of immune response and inflammation. The purpose of this study was to investigate whether anti-IL-6 monoclonal receptor antibody (IL-6RA) could ameliorate radiation-induced lung injury in mice.

Methods

BALB/cAnNCrj mice having received thoracic irradiation of 21 Gy were injected intraperitoneally with IL-6RA (MR16-1) or control rat IgG twice, immediately and seven days after irradiation. Enzyme-linked immunosorbent assay was used to examine the plasma level of IL-6 and serum amyloid A (SAA). Lung injury was assessed by histological staining with haematoxylin and eosin or Azan, measuring lung weight, and hydroxyproline.

Results

The mice treated with IL-6RA did not survive significantly longer than the rat IgG control. We observed marked up-regulation of IL-6 in mice treated with IL-6RA 150 days after irradiation, whereas IL-6RA temporarily suppressed early radiation-induced increase in the IL-6 release level. Histopathologic assessment showed no differences in lung section or lung weight between mice treated with IL-6RA and control.

Conclusions

Our findings suggest that early treatment with IL-6RA after irradiation alone does not protect against radiation-induced lung injury.
Appendix
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Metadata
Title
Early administration of IL-6RA does not prevent radiation-induced lung injury in mice
Authors
Toshiyuki Ogata
Hideya Yamazaki
Teruki Teshima
Ayaka Kihara
Yuko Suzumoto
Takehiro Inoue
Norihiro Nishimoto
Nariaki Matsuura
Publication date
01-12-2010
Publisher
BioMed Central
Published in
Radiation Oncology / Issue 1/2010
Electronic ISSN: 1748-717X
DOI
https://doi.org/10.1186/1748-717X-5-26

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