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Head & Face Medicine
Published in: Head & Face Medicine 1/2014

Open Access 01-12-2014 | Research

Smoothened gene alterations in keratocystic odontogenic tumors

Authors: Zhang Rui, Peng Li-Ying, Qu Jia-Fei, Hong Ying-Ying, Chen Feng, Li Tie-Jun

Published in: Head & Face Medicine | Issue 1/2014

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Abstract

Background

It has been widely demonstrated that the hedgehog pathway is strongly associated with basal cell carcinoma of the skin (NBCCS). To assess potential DNA alterations related to keratocystic odontogenic tumors (KCOTs), we sequenced smoothened (SMO) genes in 12 sporadic KCOTs.

Methods

Polymerase chain reaction (PCR), capillary electrophoresis and dideoxy chain-termination sequencing were used to examine potential DNA alterations in sporadic KCOTs.

Results

Five alterations in SMO genes were detected. Four of these mutations consisted of two synonymous and three missense mutations; two of which have not been reported to date (c.T776A, c.T1281G).

Conclusions

SMO genes may play an important role in the sonic hedgehog (SHH) pathway and could also be responsible for generating KCOTs and NBCCS. However, their influence on SHH signaling remains to be elucidated.
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Metadata
Title
Smoothened gene alterations in keratocystic odontogenic tumors
Authors
Zhang Rui
Peng Li-Ying
Qu Jia-Fei
Hong Ying-Ying
Chen Feng
Li Tie-Jun
Publication date
01-12-2014
Publisher
BioMed Central
Published in
Head & Face Medicine / Issue 1/2014
Electronic ISSN: 1746-160X
DOI
https://doi.org/10.1186/1746-160X-10-36

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