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Diagnostic Pathology
Published in: Diagnostic Pathology 1/2013

Open Access 01-12-2013 | Research

Identification of aberrant microRNA expression pattern in pediatric gliomas by microarray

Authors: Fatao Liu, Yuyu Xiong, Yang Zhao, Liming Tao, Zhou Zhang, Hong Zhang, Yun Liu, Guoyin Feng, Baojie Li, Lin He, Jie Ma, Shengying Qin, Yifeng Yang

Published in: Diagnostic Pathology | Issue 1/2013

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Abstract

Background

Brain tumor remains the leading cause of disease-related death in children. Many studies have focused on the complex biological process involved in pediatric brain tumors but little is know about the possible role of microRNAs in the genesis of these tumors.

Methods

In this study, we used a microRNA microarray assay to study the expression pattern of microRNAs in pediatric gliomas and matched normal tissues.

Results

We found 40 differentially expressed microRNAs, among which miR-1321, miR-513b, miR-769-3p were found be related to cancer genesis for the first time. The expression of selected microRNAs were then confirmed by qRT-PCR. Furthermore, GO and pathway analysis showed that the target genes of the 40 differentially expressed microRNAs were significantly enriched in nervous system-related and tumor-related biological processes and signaling pathways. Additionally, an apoptosis-related network of microRNA–mRNA interaction, representing the critical microRNAs and their targets, was constructed based on microRNA status.

Conclusions

In the present study we identified the changed expression pattern of microRNAs in pediatric gliamas. Our study also provides a better understanding of pediatric brain tumor biology and may assist in the development of less toxic therapies and in the search for better markers for disease stratification.

Virtual slides

The virtual slide(s) for this article can be found here: http://​www.​diagnosticpathol​ogy.​diagnomx.​eu/​vs/​1323049861105720​
Appendix
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Metadata
Title
Identification of aberrant microRNA expression pattern in pediatric gliomas by microarray
Authors
Fatao Liu
Yuyu Xiong
Yang Zhao
Liming Tao
Zhou Zhang
Hong Zhang
Yun Liu
Guoyin Feng
Baojie Li
Lin He
Jie Ma
Shengying Qin
Yifeng Yang
Publication date
01-12-2013
Publisher
BioMed Central
Published in
Diagnostic Pathology / Issue 1/2013
Electronic ISSN: 1746-1596
DOI
https://doi.org/10.1186/1746-1596-8-158

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