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Virology Journal
Published in: Virology Journal 1/2011

Open Access 01-12-2011 | Methodology

Infectious alphavirus production from a simple plasmid transfection

Authors: Jordan J Steel, Brittney R Henderson, Siddhi BC Lama, Ken E Olson, Brian J Geiss

Published in: Virology Journal | Issue 1/2011

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Abstract

We have developed a new method for producing infectious double subgenomic alphaviruses from plasmids transfected into mammalian cells. A double subgenomic Sindbis virus (TE3'2J) was transcribed from a cytomegalovirus PolII promoter, which results in the production of infectious virus. Transfection of as little as 125 ng of plasmid is able to produce 1 × 108 plaque forming units/ml (PFU/ml) of infectious virus 48 hours post-transfection. This system represents a more efficient method for producing recombinant Sindbis viruses.
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Metadata
Title
Infectious alphavirus production from a simple plasmid transfection
Authors
Jordan J Steel
Brittney R Henderson
Siddhi BC Lama
Ken E Olson
Brian J Geiss
Publication date
01-12-2011
Publisher
BioMed Central
Published in
Virology Journal / Issue 1/2011
Electronic ISSN: 1743-422X
DOI
https://doi.org/10.1186/1743-422X-8-356

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