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Virology Journal
Published in: Virology Journal 1/2011

Open Access 01-12-2011 | Research

Characterization of the duck enteritis virus UL55 protein

Authors: Ying Wu, Anchun Cheng, Mingshu Wang, Shunchuan Zhang, Dekang Zhu, Renyong Jia, Qihui Luo, Zhengli Chen, Xiaoyue Chen

Published in: Virology Journal | Issue 1/2011

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Abstract

Background

Characteration of the newly identified duck enteritis virus UL55 gene product has not been reported yet. Knowledge of the protein UL55 can provide useful insights about its function.

Results

The newly identified duck enteritis virus UL55 gene was about 561 bp, it was amplified and digested for construction of a recombinant plasmid pET32a(+)/UL55 for expression in Escherichia coli. SDS-PAGE analysis revealed the recombinant protein UL55(pUL55) was overexpressed in Escherichia coli BL21 host cells after induction by 0.2 mM IPTG at 37°C for 4 h and aggregated as inclusion bodies. The denatured protein about 40 KDa named pUL55 was purified by washing five times, and used to immune rabbits for preparation of polyclonal antibody. The prepared polyclonal antibody against pUL55 was detected and determined by Agar immundiffusion and Neutralization test. The results of Wstern blotting assay and intracellular analysis revealed that pUL55 was expressed most abundantly during the late phase of replication and mainly distributed in cytoplasm in duck enteritis virus infected cells.

Conclusions

In this study, the duck enteritis virus UL55 protein was successfully expressed in prokaryotic expression system. Besides, we have prepared the polyclonal antibody against recombinant prtein UL55, and characterized some properties of the duck enteritis virus UL55 protein for the first time. The research will be useful for further functional analysis of this gene.
Appendix
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Metadata
Title
Characterization of the duck enteritis virus UL55 protein
Authors
Ying Wu
Anchun Cheng
Mingshu Wang
Shunchuan Zhang
Dekang Zhu
Renyong Jia
Qihui Luo
Zhengli Chen
Xiaoyue Chen
Publication date
01-12-2011
Publisher
BioMed Central
Published in
Virology Journal / Issue 1/2011
Electronic ISSN: 1743-422X
DOI
https://doi.org/10.1186/1743-422X-8-256

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