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Open Access 01-12-2010 | Research

The cross-reactivity of the enterovirus 71 to human brain tissue and identification of the cross-reactivity related fragments

Authors: Chun Shi Jia, Jiang Ning Liu, Wan Bo Li, Chun Mei Ma, Shu Zhu Lin, Yi Hao, Xue Zhong Gao, Xiao Lin Liu, Yan Feng Xu, Lian Feng Zhang, Chuan Qin

Published in: Virology Journal | Issue 1/2010

Abstract

Background

EV71 occasionally cause a series of severe neurological symptoms, including aseptic meningitis, encephalitis, and poliomyelitis-like paralysis. However, the neurological destruction mechanism was remained to be clarified. This study described the cross reaction between EV71 induced IgG and human brain tissue.

Results

Cross reaction of the IgG from 30 EV71 infected patients' sera to human tissues of cerebra was observed, which suggested that some EV71 antigens could induce IgG cross-reactivity to human cerebra. To identify the regions of EV71 virus that containing above antigens, the polypeptide of virus was divided into 19 peptides by expression in prokaryotes cell. Mouse anti-sera of these peptides was prepared and applied in immunohistochemical staining with human adult and fetus brain tissue, respectively. The result indicated the 19 peptides can be classified into three groups: strong cross-reactivity, weak cross-reactivity and no cross-reactivity with human brain tissue according the cross reaction activity. Then, the increased Blood Brain Barrier (BBB) permeability and permits IgG entry in neonatal mice after EV71 infection was determined.

Conclusion

EV71 induced IgG could enter BBB and cross-reacted with brain tissue in EV71 infected neonatal mice, and then the peptides of EV71 that could induce cross-reactivity with brain tissue were identified, which should be avoided in future vaccine designing.

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Title: The cross-reactivity of the enterovirus 71 to human brain tissue and identification of the cross-reactivity related fragments
Authors: Chun Shi Jia
Jiang Ning Liu
Wan Bo Li
Chun Mei Ma
Shu Zhu Lin
Yi Hao
Xue Zhong Gao
Xiao Lin Liu
Yan Feng Xu
Lian Feng Zhang
Chuan Qin
Publication date: 01-12-2010
Publisher: BioMed Central
Published in: Virology Journal / Issue 1/2010
Electronic ISSN: 1743-422X
DOI: https://doi.org/10.1186/1743-422X-7-47

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The cross-reactivity of the enterovirus 71 to human brain tissue and identification of the cross-reactivity related fragments

Abstract

Background

Results

Conclusion

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Conclusion

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