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Published in: Virology Journal 1/2010

Open Access 01-12-2010 | Short report

Rapid detection of porcine circovirus type 2 using a TaqMan-based real-time PCR

Authors: Kai Zhao, Fangting Han, Yong Zou, Lianlong Zhu, Chunhua Li, Yan Xu, Chunling Zhang, Furong Tan, Jinbin Wang, Shiru Tao, Xizhong He, Zongqing Zhou, Xueming Tang

Published in: Virology Journal | Issue 1/2010

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Abstract

Porcine circovirus type 2 (PCV2) and the associated disease postweaning multisystemic wasting syndrome (PMWS) have caused heavy losses in global agriculture in recent decades. Rapid detection of PCV2 is very important for the effective prophylaxis and treatment of PMWS. To establish a sensitive, specific assay for the detection and quantitation of PCV2, we designed and synthesized specific primers and a probe in the open reading frame 2. The assay had a wide dynamic range with excellent linearity and reliable reproducibility, and detected between 102 and 1010 copies of the genomic DNA per reaction. The coefficient of variation for Ct values varied from 0.59% to 1.05% in the same assay and from 1.9% to 4.2% in 10 different assays. The assay did not cross-react with porcine circovirus type 1, porcine reproductive and respiratory, porcine epidemic diarrhea, transmissible gastroenteritis of pigs and rotavirus. The limits of detection and quantitation were 10 and 100 copies, respectively. Using the established real-time PCR system, 39 of the 40 samples we tested were detected as positive.
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Metadata
Title
Rapid detection of porcine circovirus type 2 using a TaqMan-based real-time PCR
Authors
Kai Zhao
Fangting Han
Yong Zou
Lianlong Zhu
Chunhua Li
Yan Xu
Chunling Zhang
Furong Tan
Jinbin Wang
Shiru Tao
Xizhong He
Zongqing Zhou
Xueming Tang
Publication date
01-12-2010
Publisher
BioMed Central
Published in
Virology Journal / Issue 1/2010
Electronic ISSN: 1743-422X
DOI
https://doi.org/10.1186/1743-422X-7-374

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