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Open Access 01-12-2010 | Research

In Vitro inhibitory activity of Alpinia katsumadai extracts against influenza virus infection and hemagglutination

Authors: Hyung-Jun Kwon, Ha-Hyun Kim, So Young Yoon, Young Bae Ryu, Jong Sun Chang, Kyoung-Oh Cho, Mun-Chual Rho, Su-Jin Park, Woo Song Lee

Published in: Virology Journal | Issue 1/2010

Abstract

Background

Alpinia katsumadai (AK) extracts and fractions were tested for in vitro antiviral activities against influenza virus type A, specially human A/PR/8/34 (H1N1) and avian A/Chicken/Korea/MS96/96 (H9N2), by means of time-of-addition experiments; pre-treatment, simultaneous treatment, and post treatment.

Results

In pre-treatment assay, the AK extracts and AK fractions did not show significant antiviral activity. During the simultaneous treatment assay, one AK extract and five AK fractions designated as AK-1 to AK-3, AK-5, AK-10, and AK-11 showed complete inhibition of virus infectivity against A/PR/8/34 (H1N1) and A/Chicken/Korea/MS96/96 (H9N2). The 50% effective inhibitory concentrations (EC₅₀) of these one AK extracts and five AK fractions with exception of the AK-9 were from 0.8 ± 1.4 to 16.4 ± 4.5 μ g/mL against A/PR/8/34 (H1N1). The two AK extracts and three AK fractions had EC₅₀ values ranging from <0.39 ± 0.4 to 2.3 ± 3.6 μ g/mL against A/Chicken/Korea/MS96/96 (H9N2). By the hemagglutination inhibition (HI) assay, the two AK extracts and five AK fractions completely inhibited viral adsorption onto chicken RBCs at less than 100 μ g/mL against both A/PR/8/34 (H1N1) and A/Chicken/Korea/MS96/96 (H9N2). Interestingly, only AK-3 was found with inhibition for both viral attachment and viral replication after showing extended antiviral activity during the post treatment assay and quantitative real-time PCR.

Conclusions

These results suggest that AK extracts and fractions had strong anti-influenza virus activity that can inhibit viral attachment and/or viral replication, and may be used as viral prophylaxis.

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Title: In Vitro inhibitory activity of Alpinia katsumadai extracts against influenza virus infection and hemagglutination
Authors: Hyung-Jun Kwon
Ha-Hyun Kim
So Young Yoon
Young Bae Ryu
Jong Sun Chang
Kyoung-Oh Cho
Mun-Chual Rho
Su-Jin Park
Woo Song Lee
Publication date: 01-12-2010
Publisher: BioMed Central
Published in: Virology Journal / Issue 1/2010
Electronic ISSN: 1743-422X
DOI: https://doi.org/10.1186/1743-422X-7-307

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