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Virology Journal
Published in: Virology Journal 1/2010

Open Access 01-12-2010 | Research

Identification of NCAM that interacts with the PHE-CoV spike protein

Authors: Wei Gao, Wenqi He, Kui Zhao, Huijun Lu, Wenzhi Ren, Chongtao Du, Keyan Chen, Yungang Lan, Deguang Song, Feng Gao

Published in: Virology Journal | Issue 1/2010

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Abstract

Background

The spike proteins of coronaviruses associate with cellular molecules to mediate infection of their target cells. The characterization of cellular proteins required for virus infection is essential for understanding viral life cycles and may provide cellular targets for antiviral therapies.

Results

We identified Neural Cell Adhesion Molecule (NCAM) as a novel interacting partner of the PHE-CoV S protein. A T7 phage display cDNA library from N2a cells was constructed, and the library was screened with the soluble PHE-CoV S glycoproteins. We used a coimmunoprecipitation assay to show that only the NCAM was a binding partner of spike protein. We found that a soluble form of anti-NCAM antibody blocked association of the PHE-CoV with N2a cells. Furthermore, double-stranded siRNA targeted against NCAM inhibited PHE-CoV infection.

Conclusions

A novel interaction was identified between NCAM and spike protein and this association is critical during PHE-CoV infection.
Appendix
Available only for authorised users
Literature
1.
Vijgen L, Keyaerts E, Lemey P, Maes P, Van Reeth K, Nauwynck H, Pensaert M, Van Ranst M: Evolutionary history of the closely related group 2 coronaviruses: porcine hemagglutinating encephalomyelitis virus, bovine coronavirus, and human coronavirus OC43. J Virol 2006, 80: 7270-7274. 10.1128/JVI.02675-05PubMedPubMedCentralCrossRef
2.
Iacono KT, Kazi L, Weiss SR: Both spike and background genes contribute to murine coronavirus neurovirulence. Journal of Virology 2006, 80: 6834-6843. 10.1128/JVI.00432-06PubMedPubMedCentralCrossRef
3.
Li WH, Moore MJ, Vasilieva N, Sui JH, Wong SK, Berne MA, Somasundaran M, Sullivan JL, Luzuriaga K, Greenough TC, et al.: Angiotensin-converting enzyme 2 is a functional receptor for the SARS coronavirus. Nature 2003, 426: 450-454. 10.1038/nature02145PubMedCrossRef
4.
Miura TA, Travanty EA, Oko L, Bielefeldt-Ohmann H, Weiss SR, Beauchemin N, Holmes KV: The spike glycoprotein of murine coronavirus MHV-JHM mediates receptor-independent infection and spread in the central nervous systems of Ceacam1a(-/-) mice. Journal of Virology 2008, 82: 755-763. 10.1128/JVI.01851-07PubMedPubMedCentralCrossRef
5.
Phillips JJ, Chu MM, Lavi E, Weiss SR: Pathogenesis of chimeric MHV4/MHV-A59 recombinant viruses: the murine coronavirus spike protein is a major determinant of neurovirulence. Journal of Virology 1999, 73: 7752-7760.PubMedPubMedCentral
6.
Jeffers SA, Tusell SM, Gillim-Ross L, Hemmila EM, Achenbach JE, Babcock GJ, Thomas WD, Thackray LB, Young MD, Mason RJ, et al.: CD209L (L-SIGN) is a receptor for severe acute respiratory syndrome coronavirus. Proceedings of the National Academy of Sciences of the United States of America 2004, 101: 15748-15753. 10.1073/pnas.0403812101PubMedPubMedCentralCrossRef
7.
Delmas B, Gelfi J, L'Haridon R, Vogel LK, Sjostrom H, Noren O, Laude H: Aminopeptidase N is a major receptor for the entero-pathogenic coronavirus TGEV. Nature 1992, 357: 417-420. 10.1038/357417a0PubMedCrossRef
8.
Tresnan DB, Levis R, Holmes KV: Feline aminopeptidase N serves as a receptor for feline, canine, porcine, and human coronaviruses in serogroup I. J Virol 1996, 70: 8669-8674.PubMedPubMedCentral
9.
Wentworth DE, Holmes KV: Molecular determinants of species specificity in the coronavirus receptor aminopeptidase N (CD13): influence of N-linked glycosylation. J Virol 2001, 75: 9741-9752. 10.1128/JVI.75.20.9741-9752.2001PubMedPubMedCentralCrossRef
10.
Yeager CL, Ashmun RA, Williams RK, Cardellichio CB, Shapiro LH, Look AT, Holmes KV: Human aminopeptidase N is a receptor for human coronavirus 229E. Nature 1992, 357: 420-422. 10.1038/357420a0PubMedCrossRef
11.
Andries K, Pensaert MB: Immunofluorescence studies on the pathogenesis of hemagglutinating encephalomyelitis virus infection in pigs after oronasal inoculation. Am J Vet Res 1980, 41: 1372-1378.PubMed
12.
Yagami K, Hirai K, Hirano N: Pathogenesis of haemagglutinating encephalomyelitis virus (HEV) in mice experimentally infected by different routes. J Comp Pathol 1986, 96: 645-657. 10.1016/0021-9975(86)90061-7PubMedCrossRef
13.
Mengeling WL, Boothe AD, Ritchie AE: Characteristics of a coronavirus (strain 67N) of pigs. Am J Vet Res 1972, 33: 297-308.PubMed
14.
Hirano N, Ono K, Takasawa H, Murakami T, Haga S: Replication and plaque formation of swine hemagglutinating encephalomyelitis virus (67N) in swine cell line, SK-K culture. J Virol Methods 1990, 27: 91-100. 10.1016/0166-0934(90)90149-APubMedCrossRef
15.
Yao L, Yin J, Zhang X, Liu Q, Li J, Chen L, Zhao Y, Gong P, Liu C: Cryptosporidium parvum: identification of a new surface adhesion protein on sporozoite and oocyst by screening of a phage-display cDNA library. Exp Parasitol 2007, 115: 333-338. 10.1016/j.exppara.2006.09.018PubMedCrossRef
16.
Prabhat P, Gan Z, Chao J, Ram S, Vaccaro C, Gibbons S, Ober RJ, Ward ES: Elucidation of intracellular recycling pathways leading to exocytosis of the Fc receptor, FcRn, by using multifocal plane microscopy. Proc Natl Acad Sci USA 2007, 104: 5889-5894. 10.1073/pnas.0700337104PubMedPubMedCentralCrossRef
17.
Gonzalez G, Pfannes L, Brazas R, Striker R: Selection of an optimal RNA transfection reagent and comparison to electroporation for the delivery of viral RNA. J Virol Methods 2007, 145: 14-21. 10.1016/j.jviromet.2007.04.013PubMedPubMedCentralCrossRef
18.
Gupte J, Cutler G, Chen JL, Tian H: Elucidation of signaling properties of vasopressin receptor-related receptor 1 by using the chimeric receptor approach. Proc Natl Acad Sci USA 2004, 101: 1508-1513. 10.1073/pnas.0308250100PubMedPubMedCentralCrossRef
19.
Rondou P, Haegeman G, Vanhoenacker P, Van Craenenbroeck K: BTB Protein KLHL12 targets the dopamine D4 receptor for ubiquitination by a Cul3-based E3 ligase. J Biol Chem 2008, 283: 11083-11096. 10.1074/jbc.M708473200PubMedPubMedCentralCrossRef
20.
Auer S, Lappalainen RS, Skottman H, Suuronen R, Narkilahti S, Vikholm-Lundin I: An antibody surface for selective neuronal cell attachment. J Neurosci Methods 2010, 186: 72-76. 10.1016/j.jneumeth.2009.11.002PubMedCrossRef
21.
Johnson JP: Cell adhesion molecules of the immunoglobulin supergene family and their role in malignant transformation and progression to metastatic disease. Cancer Metastasis Rev 1991, 10: 11-22. 10.1007/BF00046840PubMedCrossRef
22.
Zhang Y, Yeh J, Richardson PM, Bo X: Cell adhesion molecules of the immunoglobulin superfamily in axonal regeneration and neural repair. Restor Neurol Neurosci 2008, 26: 81-96.PubMed
23.
Walsh FS, Doherty P: Neural cell adhesion molecules of the immunoglobulin superfamily: role in axon growth and guidance. Annu Rev Cell Dev Biol 1997, 13: 425-456. 10.1146/annurev.cellbio.13.1.425PubMedCrossRef
24.
Dalva MB, McClelland AC, Kayser MS: Cell adhesion molecules: signalling functions at the synapse. Nature Reviews Neuroscience 2007, 8: 206-220. 10.1038/nrn2075PubMedCrossRef
25.
Hirano N, Tohyama K, Taira H: Spread of swine hemagglutinating encephalomyelitis virus from peripheral nerves to the CNS. Adv Exp Med Biol 1998, 440: 601-607.PubMedCrossRef
26.
Lain E, Carnejac S, Escher P, Wilson MC, Lomo T, Gajendran N, Brenner HR: A Novel Role for Embigin to Promote Sprouting of Motor Nerve Terminals at the Neuromuscular Junction. Journal of Biological Chemistry 2009, 284: 8921-8930.CrossRef
27.
Sanes JR, Schachner M, Covault J: Expression of several adhesive macromolecules (N-CAM, L1, J1, NILE, uvomorulin, laminin, fibronectin, and a heparan sulfate proteoglycan) in embryonic, adult, and denervated adult skeletal muscle. J Cell Biol 1986, 102: 420-431. 10.1083/jcb.102.2.420PubMedCrossRef
28.
Vlasak R, Luytjes W, Spaan W, Palese P: Human and bovine coronaviruses recognize sialic acid-containing receptors similar to those of influenza C viruses. Proc Natl Acad Sci USA 1988, 85: 4526-4529. 10.1073/pnas.85.12.4526PubMedPubMedCentralCrossRef
29.
Schultze B, Herrler G: Bovine coronavirus uses N-acetyl-9-O -acetylneuraminic acid as a receptor determinant to initiate the infection of cultured cells. J Gen Virol 1992,73(Pt 4):901-906. 10.1099/0022-1317-73-4-901PubMedCrossRef
30.
Rogers GN, Herrler G, Paulson JC, Klenk HD: Influenza C virus uses 9-O-acetyl-N-acetylneuraminic acid as a high affinity receptor determinant for attachment to cells. J Biol Chem 1986, 261: 5947-5951.PubMed
31.
Schultze B, Gross HJ, Brossmer R, Klenk HD, Herrler G: Hemagglutinating encephalomyelitis virus attaches to N-acetyl-9-O-acetylneuraminic acid-containing receptors on erythrocytes: comparison with bovine coronavirus and influenza C virus. Virus Res 1990, 16: 185-194. 10.1016/0168-1702(90)90022-4PubMedCrossRef
32.
Zeng Q, Langereis MA, van Vliet AL, Huizinga EG, de Groot RJ: Structure of coronavirus hemagglutinin-esterase offers insight into corona and influenza virus evolution. Proc Natl Acad Sci USA 2008, 105: 9065-9069. 10.1073/pnas.0800502105PubMedPubMedCentralCrossRef
33.
Murphy JA, Hartwick AT, Rutishauser U, Clarke DB: Endogenous polysialylated neural cell adhesion molecule enhances the survival of retinal ganglion cells. Invest Ophthalmol Vis Sci 2009, 50: 861-869. 10.1167/iovs.08-2334PubMedCrossRef
34.
Arellano JI, DeFelipe J, Munoz A: PSA-NCAM immunoreactivity in chandelier cell axon terminals of the human temporal cortex. Cereb Cortex 2002, 12: 617-624. 10.1093/cercor/12.6.617PubMedCrossRef
35.
Bonfanti L: PSA-NCAM in mammalian structural plasticity and neurogenesis. Prog Neurobiol 2006, 80: 129-164. 10.1016/j.pneurobio.2006.08.003PubMedCrossRef
36.
Barton ES, Connolly JL, Forrest JC, Chappell JD, Dermody TS: Utilization of sialic acid as a coreceptor enhances reovirus attachment by multistep adhesion strengthening. J Biol Chem 2001, 276: 2200-2211. 10.1074/jbc.M004680200PubMedCrossRef
37.
Quiroga MA, Cappuccio J, Pineyro P, Basso W, More G, Kienast M, Schonfeld S, Cancer JL, Arauz S, Pintos ME, et al.: Hemagglutinating encephalomyelitis coronavirus infection in pigs, Argentina. Emerg Infect Dis 2008, 14: 484-486. 10.3201/eid1403.070825PubMedPubMedCentralCrossRef
38.
Barbeau D, Liang JJ, Robitalille Y, Quirion R, Srivastava LK: Decreased expression of the embryonic form of the neural cell adhesion molecule in schizophrenic brains. Proc Natl Acad Sci USA 1995, 92: 2785-2789. 10.1073/pnas.92.7.2785PubMedPubMedCentralCrossRef
39.
Valenciano Clavel L: [Slow virus infections of the nervous system]. Rev Sanid Hig Publica (Madr) 1973, 47: 807-818.
40.
Lentz TL, Burrage TG, Smith AL, Tignor GH: The acetylcholine receptor as a cellular receptor for rabies virus. Yale J Biol Med 1983, 56: 315-322.PubMedPubMedCentral
Metadata
Title
Identification of NCAM that interacts with the PHE-CoV spike protein
Authors
Wei Gao
Wenqi He
Kui Zhao
Huijun Lu
Wenzhi Ren
Chongtao Du
Keyan Chen
Yungang Lan
Deguang Song
Feng Gao
Publication date
01-12-2010
Publisher
BioMed Central
Published in
Virology Journal / Issue 1/2010
Electronic ISSN: 1743-422X
DOI
https://doi.org/10.1186/1743-422X-7-254

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