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Virology Journal

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Open Access 01-12-2010 | Research

Monoclonal antibody induced with inactived EV71-Hn2 virus protects mice against lethal EV71-Hn2 virus infection

Authors: Guo-hui Chang, Yan-jun Luo, Xiao-yan Wu, Bing-yin Si, Lei Lin, Qing-yu Zhu

Published in: Virology Journal | Issue 1/2010

Abstract

Background

Enterovirus 71 (EV71) is a viral pathogen that belongs to the Picornaviridae family, EV71-infected children can develop severe neurological complications leading to rapid clinical deterioration and death.

Results

In this study, several monoclonal antibodies (MAbs) were produced by immunizing mice with the inactived EV71 Henan (Hn2) virus strain. The isolated MAbs were characterised by in vitro neutralizing analysis and peptide ELISA. ELISA assay showed that the neutralizing monoclonal antibody 4E8 specifically reacted with synthetic peptides which contain amino acid 240-250 and 250-260 of EV71 VP1. The in vivo protection assay showed that 4E8 can protect two-day-old BALB/c mice against the lethal challenge of EV71 virus.

Conclusion

The MAb 4E8 could be a promising candidate to be humanized and used for treatment of EV71 infection.

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Title: Monoclonal antibody induced with inactived EV71-Hn2 virus protects mice against lethal EV71-Hn2 virus infection
Authors: Guo-hui Chang
Yan-jun Luo
Xiao-yan Wu
Bing-yin Si
Lei Lin
Qing-yu Zhu
Publication date: 01-12-2010
Publisher: BioMed Central
Published in: Virology Journal / Issue 1/2010
Electronic ISSN: 1743-422X
DOI: https://doi.org/10.1186/1743-422X-7-106

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Monoclonal antibody induced with inactived EV71-Hn2 virus protects mice against lethal EV71-Hn2 virus infection

Abstract

Background

Results

Conclusion

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