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Virology Journal
Published in: Virology Journal 1/2008

Open Access 01-12-2008 | Short report

Transient expression of homologous hairpin RNA interferes with PVY transmission by aphids

Authors: Marisol Vargas, Belén Martínez-García, José Ramón Díaz-Ruíz, Francisco Tenllado

Published in: Virology Journal | Issue 1/2008

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Abstract

Hairpin RNAs have been used to confer resistance to viruses in plants through RNA silencing. However, it has not been demonstrated that RNA silencing was effective against inoculation by aphids of non-persistently transmitted viruses, the major route of plant virus spread in nature. As a proof-of-principle strategy, we made use of Agrobacterium tumefaciens to transiently express a hairpin RNA homologous to Potato virus Y (PVY) in plant tissues. A complete and specific interference with aphid transmission of PVY was achieved by inducers of RNA silencing, as demonstrated by specific siRNAs accumulation in agroinfiltrated tissues. To our knowledge, this is the first report of successful interference with non-persistent transmission of a plant virus using RNA interference.
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Metadata
Title
Transient expression of homologous hairpin RNA interferes with PVY transmission by aphids
Authors
Marisol Vargas
Belén Martínez-García
José Ramón Díaz-Ruíz
Francisco Tenllado
Publication date
01-12-2008
Publisher
BioMed Central
Published in
Virology Journal / Issue 1/2008
Electronic ISSN: 1743-422X
DOI
https://doi.org/10.1186/1743-422X-5-42

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