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Open Access 01-12-2011 | Research

Interleukin-1 beta and neurotrophin-3 synergistically promote neurite growth in vitro

Authors: Francesco Boato, Daniel Hechler, Karen Rosenberger, Doreen Lüdecke, Eva M Peters, Robert Nitsch, Sven Hendrix

Published in: Journal of Neuroinflammation | Issue 1/2011

Abstract

Pro-inflammatory cytokines such as interleukin-1 beta (IL-1β) are considered to exert detrimental effects during brain trauma and in neurodegenerative disorders. Consistently, it has been demonstrated that IL-1β suppresses neurotrophin-mediated neuronal cell survival rendering neurons vulnerable to degeneration. Since neurotrophins are also well known to strongly influence axonal plasticity, we investigated here whether IL-1β has a similar negative impact on neurite growth. We analyzed neurite density and length of organotypic brain and spinal cord slice cultures under the influence of the neurotrophins NGF, BDNF, NT-3 and NT-4. In brain slices, only NT-3 significantly promoted neurite density and length. Surprisingly, a similar increase of neurite growth was induced by IL-1β. Additionally, both factors increased the number of brain slices displaying maximal neurite growth. Furthermore, the co-administration of IL-1β and NT-3 significantly increased the number of brain slices displaying maximal neurite growth compared to single treatments. These data indicate that these two factors synergistically stimulate two distinct aspects of neurite outgrowth, namely neurite density and neurite length from acute organotypic brain slices.

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Title: Interleukin-1 beta and neurotrophin-3 synergistically promote neurite growth in vitro
Authors: Francesco Boato
Daniel Hechler
Karen Rosenberger
Doreen Lüdecke
Eva M Peters
Robert Nitsch
Sven Hendrix
Publication date: 01-12-2011
Publisher: BioMed Central
Published in: Journal of Neuroinflammation / Issue 1/2011
Electronic ISSN: 1742-2094
DOI: https://doi.org/10.1186/1742-2094-8-183

At a glance: The STEP trials

Springer Medicine

Interleukin-1 beta and neurotrophin-3 synergistically promote neurite growth in vitro

Abstract

At a glance: The STEP trials

Springer Medicine

Abstract

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