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Open Access 01-12-2010 | Research

Decreased level of recent thymic emigrants in CD4+ and CD8+T cells from CML patients

Authors: Yangqiu Li, Suxia Geng, Qingsong Yin, Shaohua Chen, Lijian Yang, Xiuli Wu, Bo Li, Xin Du, Christian A Schmidt, Grzegorz K Przybylski

Published in: Journal of Translational Medicine | Issue 1/2010

Abstract

Background

T-cell immunodeficiency is a common feature in cancer patients, which may relate to initiation and development of tumor. Based on our previous finding, to further characterize the immune status, T cell proliferative history was analyzed in CD4+ and CD8+ T cells from chronic myeloid leukemia (CML) patients.

Methods

Quantitative analysis of δRec-ψJα signal joint T cell receptor excision circles (sjTRECs) was performed in PBMCs, CD3+, CD4+ and CD8+T cells by real-time PCR, and the analysis of 23 TRBV-D1 sjTRECs was performed by semi-nested PCR. Forty eight CML cases in chronic phase (CML-CP) were selected for this study and 17 healthy individuals served as controls.

Results

The levels of δRec-ψJα sjTRECs in PBMCs, CD3+, CD4+, and CD8+ T cells were significantly decreased in CML patients, compared with control groups. Moreover, the numbers of detectable TRBV subfamily sjTRECs, as well as the frequency of particular TRBV-BD 1 sjTRECs in patients with CML were significantly lower than those from healthy individuals.

Conclusions

We observed decreased levels of recent thymic emigrants in CD4+ and CD8+ T cells that may underlay the persistent immunodeficiency in CML patients.

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Title: Decreased level of recent thymic emigrants in CD4+ and CD8+T cells from CML patients
Authors: Yangqiu Li
Suxia Geng
Qingsong Yin
Shaohua Chen
Lijian Yang
Xiuli Wu
Bo Li
Xin Du
Christian A Schmidt
Grzegorz K Przybylski
Publication date: 01-12-2010
Publisher: BioMed Central
Published in: Journal of Translational Medicine / Issue 1/2010
Electronic ISSN: 1479-5876
DOI: https://doi.org/10.1186/1479-5876-8-47

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