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Reproductive Biology and Endocrinology
Published in: Reproductive Biology and Endocrinology 1/2007

Open Access 01-12-2007 | Research

Regulation of surfactant protein D in the rodent prostate

Authors: Rebecca E Oberley, Kelli L Goss, Amado A Quintar, Cristina A Maldonado, Jeanne M Snyder

Published in: Reproductive Biology and Endocrinology | Issue 1/2007

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Abstract

Background

Surfactant protein D (SP-D) is an innate immune protein that is present in mucosal lined surfaces throughout the human body, including the male reproductive tract. In the present study, we characterized the regulation of SP-D expression in the mouse and rat prostate.

Methods

Real time reverse transcriptase polymerase chain reaction (RT-PCR) and immunostaining were used to characterize SP-D mRNA and protein in the mouse male reproductive tract. In order to evaluate the effects of testosterone on SP-D gene expression, we measured SP-D mRNA levels via real time RT-PCR in prostates from sham-castrated mice and castrated mice. In addition, we used a rat prostatitis model in which Escherichia coli was injected into the prostate in vivo to determine if infection influences SP-D protein levels in the prostate.

Results

We found that SP-D mRNA and protein are present throughout the mouse male reproductive tract, including in the prostate. We determined that castration increases prostate SP-D mRNA levels (~7 fold) when compared to levels in sham-castrated animals. Finally, we demonstrated that infection in the prostate results in a significant increase in SP-D content 24 and 48 hours post-infection.

Conclusion

Our results suggest that infection and androgens regulate SP-D in the prostate.
Appendix
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Metadata
Title
Regulation of surfactant protein D in the rodent prostate
Authors
Rebecca E Oberley
Kelli L Goss
Amado A Quintar
Cristina A Maldonado
Jeanne M Snyder
Publication date
01-12-2007
Publisher
BioMed Central
Published in
Reproductive Biology and Endocrinology / Issue 1/2007
Electronic ISSN: 1477-7827
DOI
https://doi.org/10.1186/1477-7827-5-42

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