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Reproductive Biology and Endocrinology
Published in: Reproductive Biology and Endocrinology 1/2013

Open Access 01-12-2013 | Research

SET protein up-regulated testosterone production in the cultured preantral follicles

Authors: Boqun Xu, Lingling Gao, Yugui Cui, Li Gao, Xue Dai, Mei Li, Yuan Zhang, Xiang Ma, Feiyang Diao, Jiayin Liu

Published in: Reproductive Biology and Endocrinology | Issue 1/2013

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Abstract

Background

We found previously that the expression of SET gene was up-regulated in polycystic ovaries. Evidences suggested that SET protein was essential for regulating both the promoter activity of CYP17A1 and the biological activity of P450c17. In this study, we explored whether SET regulated androgen production in preantral follicles.

Methods

The mouse preantral follicles were cultured in vitro. Testosterone secretion and expression of steroidogenic enzymes were observed in the preantral follicles treated in vitro by SET overexpression and knockdown.

Results

Testosterone levels in the media of the AdCMV-SET infected follicles significantly increased, and the CYP17A1 and HSD3B2 expression also significantly increased (P < 0.05). Testosterone levels in AdSiRNA-SET infected group decreased, and so did CYP17A1 and HSD3B2 expression (P < 0.05).

Conclusions

SET played a positive role in regulating ovarian androgen biosynthesis by enhancing the transcription of steroidogenic enzymes CYP17A1 and HSD3B2, which maybe contribute to the hyperandrogenism in PCOS.
Appendix
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Metadata
Title
SET protein up-regulated testosterone production in the cultured preantral follicles
Authors
Boqun Xu
Lingling Gao
Yugui Cui
Li Gao
Xue Dai
Mei Li
Yuan Zhang
Xiang Ma
Feiyang Diao
Jiayin Liu
Publication date
01-12-2013
Publisher
BioMed Central
Published in
Reproductive Biology and Endocrinology / Issue 1/2013
Electronic ISSN: 1477-7827
DOI
https://doi.org/10.1186/1477-7827-11-9

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