Skip to main content
Key Specialties
Cardiology Diabetology Endocrinology Gastroenterology Geriatrics and Gerontology Gynecology and Obstetrics Hematology Infectious Disease Internal Medicine Nephrology Neurology Oncology Pediatrics Respiratory Medicine Rheumatology Surgery
Congress Coverage
2024 ACC Congress 2023 ESMO Congress 2023 EASD Congress 2023 ERS Congress 2023 ESC Congress 2023 EHA Congress 2023 ASCO Annual Meeting
Clinical Collections
Advances in Alzheimer’s

Keynote webinar | Spotlight on sleep in brain health

LIVE: Thursday 2nd May 2024, 18:00-19:30 (CEST)

Quality sleep is essential for health. But what happens to our brains when sleep patterns are disturbed? Join our experts to explore the interplay between sleep disruption and neurological diseases, and the questions that you need to be asking your patients to help you prevent the harmful effects of sleep deprivation.
ADVANCED SEARCH
Log in
Top
Reproductive Biology and Endocrinology
Published in: Reproductive Biology and Endocrinology 1/2012

Open Access 01-12-2012 | Research

Hepatocyte growth factor regulates HLX1 gene expression to modulate HTR-8/SVneo trophoblast cells

Authors: Hai-Ying Liu, Xue-Qin Jia, Ling-Xue Gao, Yu-Yan Ma

Published in: Reproductive Biology and Endocrinology | Issue 1/2012

Login to get access

Abstract

Background

Paracrine signaling of the hepatocyte growth factor (HGF) cytokine plays an important role in survival and invasion ability of placental trophoblasts. However, the intracellular factors and biological pathways underlying these responses remain unclear.

Methods

This study investigated whether HGF affected the expression of homeobox gene HLX1, which is principally expressed in reproductive tissues and in some immune cells, and evaluated the implications of such in the HGF-induced human trophoblast cell line HTR-8/SVneo.

Results

HGF was found to up-regulate both HLX1 mRNA and protein levels. Transient transfection of small interfering RNA (siRNA) targeting HLX1 abrogated its induction by HGF. Functionally, HLX1 siRNA not only reduced the growth and invasion capacities of HTR-8/SVneo cells at the basal level, but also inhibited these responses induced by HGF treatment.

Conclusions

HLX1 is an essential downstream signaling component of HGF that leads to growth and invasiveness of trophoblast cells.
Appendix
Available only for authorised users
Literature
1.
2.
Nakamura T, Sakai K, Nakamura T, Matsumoto K: Hepatocyte growth factor twenty years on: Much more than a growth factor. J Gastroenterol Hepatol. 2011, 26 (Suppl 1): 188-202.CrossRefPubMed
3.
Uehara Y, Minowa O, Mori C, Shiota K, Kuno J, Noda T, Kitamura N: Placental defect and embryonic lethality in mice lacking hepatocyte growth factor/scatter factor. Nature. 1995, 373 (6516): 702-705. 10.1038/373702a0.CrossRefPubMed
4.
Schmidt C, Bladt F, Goedecke S, Brinkmann V, Zschiesche W, Sharpe M, Gherardi E, Birchmeier C: Scatter factor/hepatocyte growth factor is essential for liver development. Nature. 1995, 373 (6516): 699-702. 10.1038/373699a0.CrossRefPubMed
5.
Bladt F, Riethmacher D, Isenmann S, Aguzzi A, Birchmeier C: Essential role for the c-met receptor in the migration of myogenic precursor cells into the limb bud. Nature. 1995, 376 (6543): 768-771. 10.1038/376768a0.CrossRefPubMed
6.
Wolf HK, Zarnegar R, Oliver L, Michalopoulos GK: Hepatocyte growth factor in human placenta and trophoblastic disease. Am J Pathol. 1991, 138 (4): 1035-1043.PubMedCentralPubMed
7.
Saito S, Sakakura S, Enomoto M, Ichijo M, Matsumoto K, Nakamura T: Hepatocyte growth factor promotes the growth of cytotrophoblasts by the paracrine mechanism. J Biochem. 1995, 117 (3): 671-676.PubMed
8.
Clark DE, Smith SK, Sharkey AM, Sowter HM, Charnock-Jones DS: Hepatocyte growth factor/scatter factor and its receptor c-met: localisation and expression in the human placenta throughout pregnancy. J Endocrinol. 1996, 151 (3): 459-467. 10.1677/joe.0.1510459.CrossRefPubMed
9.
Aoki S, Hata T, Manabe A, Miyazaki K: Decreased maternal circulating hepatocyte growth factor (HGF) concentrations in pregnancies with small for gestational age infants. Hum Reprod. 1998, 13 (10): 2950-2953. 10.1093/humrep/13.10.2950.CrossRefPubMed
10.
Cartwright JE, Holden DP, Whitley GS: Hepatocyte growth factor regulates human trophoblast motility and invasion: a role for nitric oxide. Br J Pharmacol. 1999, 128 (1): 181-189. 10.1038/sj.bjp.0702757.PubMedCentralCrossRefPubMed
11.
Somerset DA, Li XF, Afford S, Strain AJ, Ahmed A, Sangha RK, Whittle MJ, Kilby MD: Ontogeny of hepatocyte growth factor (HGF) and its receptor (c-met) in human placenta: reduced HGF expression in intrauterine growth restriction. Am J Pathol. 1998, 153 (4): 1139-1147. 10.1016/S0002-9440(10)65658-1.PubMedCentralCrossRefPubMed
12.
Patel Y, Kim H, Rappolee DA: A role for hepatocyte growth factor during early postimplantation growth of the placental lineage in mice. Biol Reprod. 2000, 62 (4): 904-912. 10.1095/biolreprod62.4.904.CrossRefPubMed
13.
Nasu K, Sugano T, Matsui N, Narahara H, Kawano Y, Miyakawa I: Expression of hepatocyte growth factor in cultured human endometrial stromal cells is induced through a protein kinase C-dependent pathway. Biol Reprod. 1999, 60 (5): 1183-1187. 10.1095/biolreprod60.5.1183.CrossRefPubMed
14.
Rajaraman G, Murthi P, Quinn L, Brennecke SP, Kalionis B: Homeodomain protein HLX is expressed primarily in cytotrophoblast cell types in the early pregnancy human placenta. Reprod Fertil Dev. 2008, 20 (3): 357-367. 10.1071/RD07159.CrossRefPubMed
15.
Murthi P, Doherty V, Said J, Donath S, Brennecke SP, Kalionis B: Homeobox gene HLX1 expression is decreased in idiopathic human fetal growth restriction. Am J Pathol. 2006, 168 (2): 511-518. 10.2353/ajpath.2006.050637.PubMedCentralCrossRefPubMed
16.
Livak KJ, Schmittgen TD: Analysis of relative gene expression data using real-time quantitative PCR and the 2(−Delta Delta C(T)) Method. Methods. 2001, 25 (4): 402-408. 10.1006/meth.2001.1262.CrossRefPubMed
17.
Dash PR, Whitley GS, Ayling LJ, Johnstone AP, Cartwright JE: Trophoblast apoptosis is inhibited by hepatocyte growth factor through the Akt and beta-catenin mediated up-regulation of inducible nitric oxide synthase. Cell Signal. 2005, 17 (5): 571-580. 10.1016/j.cellsig.2004.09.015.CrossRefPubMed
18.
Kauma SW, Bae-Jump V, Walsh SW: Hepatocyte growth factor stimulates trophoblast invasion: a potential mechanism for abnormal placentation in preeclampsia. J Clin Endocrinol Metab. 1999, 84 (11): 4092-4096. 10.1210/jc.84.11.4092.PubMed
19.
Shin JC, Moon HB, Lee JH, Yang DE, Lee G, Lee Y, Lee JS, Kim CY, Kim SP: Influence of Hepatocyte Growth Factor on Matrix Metalloproteinase Expression in HT cell line. Korean J Obstet Gynecol. 2001, 44 (12): 2257-2262.
20.
Graham CH, Hawley TS, Hawley RG, MacDougall JR, Kerbel RS, Khoo N, Lala PK: Establishment and characterization of first trimester human trophoblast cells with extended lifespan. Exp Cell Res. 1993, 206 (2): 204-211. 10.1006/excr.1993.1139.CrossRefPubMed
21.
Shiverick KT, King A, Frank H, Whitley GS, Cartwright JE, Schneider H: Cell culture models of human trophoblast II: trophoblast cell lines--a workshop report. Placenta. 2001, 22 (Suppl A): S104-S106.CrossRefPubMed
22.
Dokras A, Gardner LM, Seftor EA, Hendrix MJ: Regulation of human cytotrophoblast morphogenesis by hepatocyte growth factor/scatter factor. Biol Reprod. 2001, 65 (4): 1278-1288. 10.1095/biolreprod65.4.1278.CrossRefPubMed
23.
Lala PK, Chakraborty C: Factors regulating trophoblast migration and invasiveness: possible derangements contributing to pre-eclampsia and fetal injury. Placenta. 2003, 24 (6): 575-587. 10.1016/S0143-4004(03)00063-8.CrossRefPubMed
24.
Kehrl JH, Deguchi Y: Potential roles for two human homeodomain containing proteins in the proliferation and differentiation of human hematopoietic progenitors. Leuk Lymphoma. 1993, 10 (3): 173-176. 10.3109/10428199309145879.CrossRefPubMed
25.
Rajaraman G, Murthi P, Leo B, Brennecke SP, Kalionis B: Homeobox gene HLX1 is a regulator of colony stimulating factor-1 dependent trophoblast cell proliferation. Placenta. 2007, 28 (10): 991-998. 10.1016/j.placenta.2007.03.011.CrossRefPubMed
26.
Castellucci M, De Matteis R, Meisser A, Cancello R, Monsurro V, Islami D, Sarzani R, Marzioni D, Cinti S, Bischof P: Leptin modulates extracellular matrix molecules and metalloproteinases: possible implications for trophoblast invasion. Mol Hum Reprod. 2000, 6 (10): 951-958. 10.1093/molehr/6.10.951.CrossRefPubMed
27.
Jovanovic M, Stefanoska I, Radojcic L, Vicovac L: Interleukin-8 (CXCL8) stimulates trophoblast cell migration and invasion by increasing levels of matrix metalloproteinase (MMP)2 and MMP9 and integrins alpha5 and beta1. Reproduction. 2010, 139 (4): 789-798. 10.1530/REP-09-0341.CrossRefPubMed
28.
Rajaraman G, Murthi P, Brennecke SP, Kalionis B: Homeobox gene HLX is a regulator of HGF/c-met-mediated migration of human trophoblast-derived cell lines. Biol Reprod. 2010, 83 (4): 676-683. 10.1095/biolreprod.109.078634.CrossRefPubMed
Metadata
Title
Hepatocyte growth factor regulates HLX1 gene expression to modulate HTR-8/SVneo trophoblast cells
Authors
Hai-Ying Liu
Xue-Qin Jia
Ling-Xue Gao
Yu-Yan Ma
Publication date
01-12-2012
Publisher
BioMed Central
Published in
Reproductive Biology and Endocrinology / Issue 1/2012
Electronic ISSN: 1477-7827
DOI
https://doi.org/10.1186/1477-7827-10-83

Other articles of this Issue 1/2012

Reproductive Biology and Endocrinology 1/2012 Go to the issue

Research

Effects of estradiol and FSH on leptin levels in women with suppressed pituitary

Research

Androgen receptor-beta mRNA levels in different tissues in breeding and post-breeding male and female sticklebacks, Gasterosteus aculeatus

Research

Addition of low dose hCG to rFSh benefits older women during ovarian stimulation for IVF

Research

Expression of antiapoptosis gene survivin in luteinized ovarian granulosa cells of women undergoing IVF or ICSI and embryo transfer: clinical correlations

Research

Improvement of ovarian response and oocyte quality of aged female by administration of bone morphogenetic protein-6 in a mouse model

Research

The efficacy of ultrasound treatment as a reversible male contraceptive in the rhesus monkey