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Reproductive Biology and Endocrinology
Published in: Reproductive Biology and Endocrinology 1/2012

Open Access 01-12-2012 | Research

Differential expression of extracellular matrix components in the Fallopian tubes throughout the menstrual cycle

Authors: Patricia S Diaz, Paula A Solar, Natalia E Juica, Pedro A Orihuela, Hugo Cardenas, Myron Christodoulides, Renato Vargas, Luis A Velasquez

Published in: Reproductive Biology and Endocrinology | Issue 1/2012

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Abstract

Background

One of the unique characteristics of the female genital tract is the extensive tissue remodeling observed throughout the menstrual cycle. Multiple components of the extracellular matrix take part in this tissue rebuilding; however, the individual components involved have not been identified.

Methods

In the present study, the expression of extracellular matrix proteins and selected matrix metalloproteinase (MMP) activities in Fallopian tubes (FT) throughout the menstrual cycle were examined by PCR array, immunocytochemistry, zymography and bioinformatics.

Results

Of the eighty-four genes analyzed, eighty-three were expressed in the FT during at least one stage of the menstrual cycle. We observed a significant increase (>/=2-fold) in ADAMTS1, ADAMTS13, COL7A1, MMP3, MMP9, PECAM1, and THBS3 in the periovulatory phase compared to the follicular phase. Meanwhile, we observed a significant decrease (>/= 2-fold) in COL7A1, ICAM1, ITGA8, MMP16, MMP9, CLEC3B, SELE and TIMP2 in the lutheal phase compared to the periovulatory phase. Immunocytochemistry showed that MMP-3 and MMP-9 were localized in the endosalpinx during all phases of the menstrual cycle. Gelatin zymograms detected non-cycle-dependent protease activity.

Conclusions

Several extracellular matrix components were regulated throughout the menstrual cycle in a cyclic pattern, suggesting a possible steroid regulation and a role in tissue remodeling and FT functions.
Appendix
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Metadata
Title
Differential expression of extracellular matrix components in the Fallopian tubes throughout the menstrual cycle
Authors
Patricia S Diaz
Paula A Solar
Natalia E Juica
Pedro A Orihuela
Hugo Cardenas
Myron Christodoulides
Renato Vargas
Luis A Velasquez
Publication date
01-12-2012
Publisher
BioMed Central
Published in
Reproductive Biology and Endocrinology / Issue 1/2012
Electronic ISSN: 1477-7827
DOI
https://doi.org/10.1186/1477-7827-10-56

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