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Reproductive Biology and Endocrinology
Published in: Reproductive Biology and Endocrinology 1/2012

Open Access 01-12-2012 | Research

Relationship between quantity of IFNT estimated by IFN-stimulated gene expression in peripheral blood mononuclear cells and bovine embryonic mortality after AI or ET

Authors: Shuichi Matsuyama, Takatoshi Kojima, Satoru Kato, Koji Kimura

Published in: Reproductive Biology and Endocrinology | Issue 1/2012

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Abstract

Background

Interferon tau (IFNT), which is secreted into the uterine cavity during the maternal recognition period (MRP), is a key factor for establishment of pregnancy. The present study aims to clarify the relationship between the ability of a bovine conceptus to produce IFNT during the MRP and the conceptus's ability to establish pregnancy.

Methods

In the first experiment, IFNT (0, 500, or 1000 micrograms) was administered into the uterine horn ipsilateral to the CL 16 or 17 d after standing estrus, and mRNA levels of IFN-stimulated gene 15-kDa protein (ISG15) and Mx2 in peripheral blood mononuclear cells (PBMCs) were determined. In the second experiment, we investigated ISG15 mRNA expression in PBMCs during the MRP in cattle after either artificial insemination (AI) or embryo transfer (ET).

Results

Intrauterine administration of IFNT stimulated ISG15 and Mx2 gene expressions in PBMCs in cattle, and there was a positive correlation between the expressions of peripheral markers and the quantity of IFNT administered. In pregnant and normal interestrous interval (< 25 d) cattle (nIEI cattle), expression levels of the ISG15 gene showed similar patterns after AI and ET, and ISG15 mRNA expression was increased in pregnant cattle but unchanged in nIEI cattle. In contrast, ISG15 gene expression in extended interestrous interval (greater than or equal to 25 d) cattle (eIEI cattle) differed after ET compared with AI. In eIEI cattle after ET, ISG15 gene expression increased, such that the value on day 18 was intermediate between those of pregnant and nIEI cattle. In eIEI cattle after AI, ISG15 gene expression did not increase throughout the observation period.

Conclusions

The results of the current study indicate that the quantity of conceptus-derived IFNT can be estimated by measuring ISG15 mRNA levels in PBMCs from cattle. Using this approach, we demonstrate that ISG15 gene expression during the MRP in eIEI cattle differed after ET compared with AI. In addition, the modest increase in ISG15 gene expression in eIEI cattle after ET suggests that late embryo losses were due to delayed or insufficient growth of the conceptus during the MRP in cattle.
Appendix
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Literature
1.
Hernandez-Ledezma JJ, Sikes JD, Murphy CN, Watson AJ, Schultz GA, Roberts RM: Expression of bovine trophoblast interferon in conceptuses derived by in vitro techniques. Biol Reprod. 1992, 47 (3): 374-380. 10.1095/biolreprod47.3.374.CrossRefPubMed
2.
Garrett JE, Geisert RD, Zavy MT, Morgan GL: Evidence for maternal regulation of early conceptus growth and development in beef cattle. J Reprod Fertil. 1988, 84 (2): 437-446. 10.1530/jrf.0.0840437.CrossRefPubMed
3.
Mann GE, Fray MD, Lamming GE: Effects of time of progesterone supplementation on embryo development and interferon-tau production in the cow. Vet J. 2006, 171 (3): 500-503. 10.1016/j.tvjl.2004.12.005.CrossRefPubMed
4.
Mann GE, Lamming GE: Relationship between maternal endocrine environment, early embryo development and inhibition of the luteolytic mechanism in cows. Reproduction. 2001, 121 (1): 175-180. 10.1530/rep.0.1210175.CrossRefPubMed
5.
Nephew KP, Cardenas H, McClure KE, Ott TL, Bazer FW, Pope WF: Effects of administration of human chorionic gonadotropin or progesterone before maternal recognition of pregnancy on blastocyst development and pregnancy in sheep. J Anim Sci. 1994, 72 (2): 453-458.PubMed
6.
Bartol FF, Roberts RM, Bazer FW, Lewis GS, Godkin JD, Thatcher WW: Characterization of proteins produced in vitro by periattachment bovine conceptuses. Biol Reprod. 1985, 32 (3): 681-693. 10.1095/biolreprod32.3.681.CrossRefPubMed
7.
Roberts RM, Cross JC, Leaman DW: Unique features of the trophoblast interferons. Pharmacol Ther. 1991, 51 (3): 329-345. 10.1016/0163-7258(91)90064-S.CrossRefPubMed
8.
Hansen TR, Kazemi M, Keisler DH, Malathy PV, Imakawa K, Roberts RM: Complex binding of the embryonic interferon, ovine trophoblast protein-1, to endometrial receptors. J Interferon Res. 1989, 9 (2): 215-225. 10.1089/jir.1989.9.215.CrossRefPubMed
9.
Spencer TE, Bazer FW: Ovine interferon tau suppresses transcription of the estrogen receptor and oxytocin receptor genes in the ovine endometrium. Endocrinology. 1996, 137 (3): 1144-1147. 10.1210/en.137.3.1144.PubMed
10.
Spencer TE, Becker WC, George P, Mirando MA, Ogle TF, Bazer FW: Ovine interferon-tau inhibits estrogen receptor up-regulation and estrogen-induced luteolysis in cyclic ewes. Endocrinology. 1995, 136 (11): 4932-4944. 10.1210/en.136.11.4932.PubMed
11.
Thatcher WW, Meyer MD, Danet-Desnoyers G: Maternal recognition of pregnancy. J Reprod Fertil Suppl. 1995, 49: 15-28.PubMed
12.
Vallet JL, Bazer FW, Fliss MF, Thatcher WW: Effect of ovine conceptus secretory proteins and purified ovine trophoblast protein-1 on interoestrous interval and plasma concentrations of prostaglandins F-2α and E and of 13,14-dihydro-15-keto prostaglandin F-2α in cyclic ewes. J Reprod Fertil. 1988, 84 (2): 493-504. 10.1530/jrf.0.0840493.CrossRefPubMed
13.
Hansen TR, Austin KJ, Perry DJ, Pru JK, Teixeira MG, Johnson GA: Mechanism of action of interferon-tau in the uterus during early pregnancy. J Reprod Fertil Suppl. 1999, 54: 329-339.PubMed
14.
Roberts RM, Cross JC, Leaman DW: Interferons as hormones of pregnancy. Endocr Rev. 1992, 13 (3): 432-452.PubMed
15.
Spencer TE, Burghardt RC, Johnson GA, Bazer FW: Conceptus signals for establishment and maintenance of pregnancy. Anim Reprod Sci. 2004, 82-83: 537-550.CrossRefPubMed
16.
Han H, Austin KJ, Rempel LA, Hansen TR: Low blood ISG15 mRNA and progesterone levels are predictive of non-pregnant dairy cows. J Endocrinol. 2006, 191 (2): 505-512. 10.1677/joe.1.07015.CrossRefPubMed
17.
Yankey SJ, Hicks BA, Carnahan KG, Assiri AM, Sinor SJ, Kodali K, Stellflug JN, Ott TL: Expression of the antiviral protein Mx in peripheral blood mononuclear cells of pregnant and bred, non-pregnant ewes. J Endocrinol. 2001, 170 (2): R7-R11. 10.1677/joe.0.170R007.CrossRefPubMed
18.
Gifford CA, Racicot K, Clark DS, Austin KJ, Hansen TR, Lucy MC, Davies CJ, Ott TL: Regulation of interferon-stimulated genes in peripheral blood leukocytes in pregnant and bred, nonpregnant dairy cows. J Dairy Sci. 2007, 90 (1): 274-280. 10.3168/jds.S0022-0302(07)72628-0.CrossRefPubMed
19.
Bott RC, Ashley RL, Henkes LE, Antoniazzi AQ, Bruemmer JE, Niswender GD, Bazer FW, Spencer TE, Smirnova NP, Anthony RV, Hansen TR: Uterine vein infusion of interferon tau (IFNT) extends luteal life span in ewes. Biol Reprod. 2010, 82 (4): 725-735. 10.1095/biolreprod.109.079467.CrossRefPubMed
20.
Oliveira JF, Henkes LE, Ashley RL, Purcell SH, Smirnova NP, Veeramachaneni DN, Anthony RV, Hansen TR: Expression of interferon (IFN)-stimulated genes in extrauterine tissues during early pregnancy in sheep is the consequence of endocrine IFN-tau release from the uterine vein. Endocrinology. 2008, 149 (3): 1252-1259.CrossRefPubMed
21.
Green JC, Okamura CS, Poock SE, Lucy MC: Measurement of interferon-tau (IFN-tau) stimulated gene expression in blood leukocytes for pregnancy diagnosis within 18-20d after insemination in dairy cattle. Anim Reprod Sci. 2010, 121 (1-2): 24-33.CrossRefPubMed
22.
Diskin MG, Sreenan JM: Fertilization and embryonic mortality rates in beef heifers after artificial insemination. J Reprod Fertil. 1980, 59 (2): 463-468. 10.1530/jrf.0.0590463.CrossRefPubMed
23.
Dunne LD, Diskin MG, Sreenan JM: Embryo and foetal loss in beef heifers between day 14 of gestation and full term. Anim Reprod Sci. 2000, 58 (1-2): 39-44. 10.1016/S0378-4320(99)00088-3.CrossRefPubMed
24.
Roche JF, Bolandl MP, McGeady TA: Reproductive wastage following artificial insemination of heifers. Vet Rec. 1981, 109 (18): 401-404. 10.1136/vr.109.18.401.CrossRefPubMed
25.
Silke V, Diskin MG, Kenny DA, Boland MP, Dillon P, Mee JF, Sreenan JM: Extent, pattern and factors associated with late embryonic loss in dairy cows. Anim Reprod Sci. 2002, 71 (1-2): 1-12. 10.1016/S0378-4320(02)00016-7.CrossRefPubMed
26.
Horan B, Mee JF, Rath M, O'Connor P, Dillon P: The effect of strain of Holstein-Friesian cow and feeding system on reproductive performance in seasonal calving milk production systems. Animal Sci. 2004, 79: 453-467.
27.
Humblot P: Use of pregnancy specific proteins and progesterone assays to monitor pregnancy and determine the timing, frequencies and sources of embryonic mortality in ruminants. Theriogenology. 2001, 56 (9): 1417-1433. 10.1016/S0093-691X(01)00644-6.CrossRefPubMed
28.
Imakawa K, Anthony RV, Kazemi M, Marotti KR, Polites HG, Roberts RM: Interferon-like sequence of ovine trophoblast protein secreted by embryonic trophectoderm. Nature. 1987, 330 (6146): 377-379. 10.1038/330377a0.CrossRefPubMed
29.
Plante C, Hansen PJ, Martinod S, Siegenthaler B, Thatcher WW, Pollard JW, Leslie MV: Effect of intrauterine and intramuscular administration of recombinant bovine interferon α1 on luteal lifespan in cattle. J Dairy Sci. 1989, 72 (7): 1859-1865. 10.3168/jds.S0022-0302(89)79304-8.CrossRefPubMed
30.
Santos JE, Thatcher WW, Chebel RC, Cerri RL, Galvao KN: The effect of embryonic death rates in cattle on the efficacy of estrus synchronization programs. Anim Reprod Sci. 2004, 82-83: 513-535.CrossRefPubMed
31.
Diskin MG, Murphy JJ, Sreenan JM: Embryo survival in dairy cows managed under pastoral conditions. Anim Reprod Sci. 2006, 96 (3-4): 297-311. 10.1016/j.anireprosci.2006.08.008.CrossRefPubMed
32.
Kimura K, Hirako M, Iwata H, Aoki M, Kawaguchi M, Seki M: Successful superovulation of cattle by a single administration of FSH in aluminum hydroxide gel. Theriogenology. 2007, 68 (4): 633-639. 10.1016/j.theriogenology.2007.02.016.CrossRefPubMed
33.
Stringfellow DA, Seidel SM: Manual of the International Embryo Transfer Society: A procedural guide and general information for the use of embryo transfer technology emphasizing sanitary procedures. 1998, Savoy, IL: International Embryo Transfer Society, 3
34.
Dochi O, Yamamoto Y, Saga H, Yoshiba N, Kano N, Maeda J, Miyata K, Yamauchi A, Tominaga K, Oda Y, Nakashima T, Inohae S: Direct transfer of bovine embryos frozen-thawed in the presence of propylene glycol or ethylene glycol under on-farm conditions in an integrated embryo transfer program. Theriogenology. 1998, 49 (5): 1051-1058. 10.1016/S0093-691X(98)00053-3.CrossRefPubMed
35.
Takenouchi N, Oshima K, Shimada K, Takahashi M: The development of a sensitive enzyme immunoassay for the determination of estrone and estradiol-17β in bovine blood plasma based on the same homologous combination with antiserum and steroid-enzyme conjugate. J Vet Med Sci. 2004, 66 (11): 1315-1321. 10.1292/jvms.66.1315.CrossRefPubMed
36.
Henricks DM, Hill JR, Dickey JF: Plasma ovarian hormone levels and fertility in beef heifers treated with melengestrol acetate (MGA). J Anim Sci. 1973, 37 (5): 1169-1175.PubMed
37.
Bazer FW, Burghardt RC, Johnson GA, Spencer TE, Wu G: Interferons and progesterone for establishment and maintenance of pregnancy: interactions among novel cell signaling pathways. Reprod Biol. 2008, 8 (3): 179-211.CrossRefPubMed
38.
Spencer TE, Johnson GA, Bazer FW, Burghardt RC: Fetal-maternal interactions during the establishment of pregnancy in ruminants. Soc Reprod Fertil Suppl. 2007, 64: 379-396.PubMed
39.
Spencer TE, Johnson GA, Bazer FW, Burghardt RC, Palmarini M: Pregnancy recognition and conceptus implantation in domestic ruminants: roles of progesterone, interferons and endogenous retroviruses. Reprod Fertil Dev. 2007, 19 (1): 65-78. 10.1071/RD06102.CrossRefPubMed
40.
Lenschow DJ, Giannakopoulos NV, Gunn LJ, Johnston C, O'Guin AK, Schmidt RE, Levine B, Virgin HWt: Identification of interferon-stimulated gene 15 as an antiviral molecule during Sindbis virus infection in vivo. J Virol. 2005, 79 (22): 13974-13983. 10.1128/JVI.79.22.13974-13983.2005.PubMedCentralCrossRefPubMed
41.
Nakaya T, Sato M, Hata N, Asagiri M, Suemori H, Noguchi S, Tanaka N, Taniguchi T: Gene induction pathways mediated by distinct IRFs during viral infection. Biochem Biophys Res Commun. 2001, 283 (5): 1150-1156. 10.1006/bbrc.2001.4913.CrossRefPubMed
42.
Herath S, Lilly ST, Fischer DP, Williams EJ, Dobson H, Bryant CE, Sheldon IM: Bacterial lipopolysaccharide induces an endocrine switch from prostaglandin F2α to prostaglandin E2 in bovine endometrium. Endocrinology. 2009, 150 (4): 1912-1920. 10.1210/en.2008-1379.PubMedCentralCrossRefPubMed
43.
Del Vecchio RP, Matsas DJ, Inzana TJ, Sponenberg DP, Lewis GS: Effect of intrauterine bacterial infusions and subsequent endometritis on prostaglandin F2α metabolite concentrations in postpartum beef cows. J Anim Sci. 1992, 70 (10): 3158-3162.PubMed
Metadata
Title
Relationship between quantity of IFNT estimated by IFN-stimulated gene expression in peripheral blood mononuclear cells and bovine embryonic mortality after AI or ET
Authors
Shuichi Matsuyama
Takatoshi Kojima
Satoru Kato
Koji Kimura
Publication date
01-12-2012
Publisher
BioMed Central
Published in
Reproductive Biology and Endocrinology / Issue 1/2012
Electronic ISSN: 1477-7827
DOI
https://doi.org/10.1186/1477-7827-10-21

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