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World Journal of Surgical Oncology

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Open Access 01-12-2011 | Research

Expression of AIB1 protein as a prognostic factor in breast cancer

Authors: Kyungji Lee, Ahwon Lee, Byung Joo Song, Chang Suk Kang

Published in: World Journal of Surgical Oncology | Issue 1/2011

Abstract

Background

AIB1 (amplified in breast cancer I) is a member of the p160 steroid receptor coactivator family. AIB1 is frequently overexpressed in breast cancer and has functions that promote oncogenesis that are independent of estrogen receptor (ER) coactivation. We investigated prognostic significance of AIB1 and relationship between AIB1 and ER, progesterone receptor (PR), androgen receptor (AR), DAX-1, and HER2.

Methods

RNA in situ hybridization (ISH) and immunohistochemical (IHC) staining for AIB1, IHC staining for ER and the progesterone receptor (PR) and IHC staining and silver in situ hybridization (SISH) for HER2 were performed for 185 breast cancer cases.

Results

A high level of expression of AIB1 mRNA was observed in 60.0% of tumors. IHC analysis detected AIB1 positivity in 47.3% of tumors, which did not correlate with AIB1 mRNA expression (p = 0.24, r = 0.10). AIB1 protein expression correlated with AR and DAX-1 expression (p = 0.01, r = 0.22 and p = 0.02, r = 0.21, respectively) but not with ER or PR expression (p = 0.14, r = -0.13 and p = 0.16, r = -0.12, respectively). AIB1 protein expression correlated with the amplification of the HER2 gene (p = 0.03, r = 0.19). In contrast to AIB1 protein expression, AIB1 mRNA expression did not correlate with AR, DAX-1, ER, and PR expression, and the amplification of the HER2 gene (p > 0.05 for all).

There were trends that strong AIB1 protein expression correlated with poorer disease free survival (p = 0.07). Strong AIB1 protein expression correlated with poorer overall survival (p = 0.04). Among the ER-negative subgroup, strong AIB1 protein expression correlated with poorer disease free survival and overall survival (p = 0.01 and p < 0.01, respectively).

Conclusions

Strong AIB1 protein expression was poor prognostic factor in breast cancer, especially in ER-negative breast cancers. Further investigation is essential to determine whether AIB1 might be effective therapeutic targets for ER-negative breast cancers.

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Title: Expression of AIB1 protein as a prognostic factor in breast cancer
Authors: Kyungji Lee
Ahwon Lee
Byung Joo Song
Chang Suk Kang
Publication date: 01-12-2011
Publisher: BioMed Central
Published in: World Journal of Surgical Oncology / Issue 1/2011
Electronic ISSN: 1477-7819
DOI: https://doi.org/10.1186/1477-7819-9-139

Keynote webinar | Spotlight on medication adherence

Springer Medicine

Expression of AIB1 protein as a prognostic factor in breast cancer

Abstract

Background

Methods

Results

Conclusions

Keynote webinar | Spotlight on medication adherence

Springer Medicine

Abstract

Background

Methods

Results

Conclusions

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