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Open Access 01-12-2010 | Research

Genomic alterations of primary tumor and blood in invasive ductal carcinoma of breast

Authors: Ja Seong Bae, Jin Soo Choi, Seung Ho Baik, Woo Chan Park, Byung Joo Song, Jeong Soo Kim, Young Lim, Sang Seol Jung

Published in: World Journal of Surgical Oncology | Issue 1/2010

Abstract

Background

Genomic alterations are important events in the origin and progression of various cancers, with DNA copy number changes associated with progression and treatment response in cancer. Array CGH is potentially useful in the identification of genomic alterations from primary tumor and blood in breast cancer patients. The aim of our study was to compare differences of DNA copy number changes in blood and tumor tissue in breast cancer.

Methods

DNA copy number changes in blood were compared to those in tumor tissue using array-comparative genomic hybridization in samples obtained from 30 breast cancer patients. The relative degree of chromosomal changes was analyzed using log2 ratios and data was validated by real-time polymerase chain reaction.

Results

Forty-six regions of gains present in more than 30% of the tissues and 70 regions of gains present in more than 30% of blood were identified. The most frequently gained region was chromosome 8q24. In total, agreement of DNA copy numbers between primary tumor and blood was minimal (Kappa = 0.138, p < 0.001).

Conclusion

Although there was only a slight agreement of DNA copy number alterations between the primary tumor and the blood samples, the blood cell copy number variation may have some clinical significance as compared to the primary tumor in IDC breast cancer patients.

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Title: Genomic alterations of primary tumor and blood in invasive ductal carcinoma of breast
Authors: Ja Seong Bae
Jin Soo Choi
Seung Ho Baik
Woo Chan Park
Byung Joo Song
Jeong Soo Kim
Young Lim
Sang Seol Jung
Publication date: 01-12-2010
Publisher: BioMed Central
Published in: World Journal of Surgical Oncology / Issue 1/2010
Electronic ISSN: 1477-7819
DOI: https://doi.org/10.1186/1477-7819-8-32

Keynote webinar | Spotlight on medication adherence

Springer Medicine

Genomic alterations of primary tumor and blood in invasive ductal carcinoma of breast

Abstract

Background

Methods

Results

Conclusion

Keynote webinar | Spotlight on medication adherence

Springer Medicine

Abstract

Background

Methods

Results

Conclusion

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