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World Journal of Surgical Oncology

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Open Access 01-12-2014 | Research

Prognostic significance of TMEM16A, PPFIA1, and FADD expression in invasive ductal carcinoma of the breast

Authors: Eun Ji Choi, Jeong A Yun, Sahrish Jabeen, Eun Kyoung Jeon, Hye Sung Won, Yoon Ho Ko, Su Young Kim

Published in: World Journal of Surgical Oncology | Issue 1/2014

Abstract

Background

11q13 region is a frequently amplified locus in human malignancies. Among the genes located in this region, FADD is one of the alleged driving genes. Because amplification is not generally confined to a single gene and amplified genes may not show increased expression, we need to evaluate clinical significance of changes occurring in 11q13 region to understand their roles in carcinogenesis. Therefore, we screened expressions of FADD and closely located genes (PPFIA1 and TMEM16A) and evaluated the expressions to find clinical significance in invasive ductal carcinoma of the breast.

Methods

Ninety-eight cases of invasive ductal carcinoma of the breast were collected. Using archival tissues resected from the cases, we built a tissue microarray and used it in immunohistochemistry. We evaluated the association of FADD, PPFIA1, and TMEM16A expression scores with clinicopathological parameters, including disease-free survival.

Results

FADD expression was associated with T stage (P = 0.046). The combined score of FADD, PPFIA1, and TMEM16A gene expressions was associated with perineural invasion (P = 0.022). Although individual gene expressions of TMEM16A, FADD, and PPFIA1 failed to show significant association with disease-free survival, combined gene expression scores did show association with disease-free survival (P = 0.034).

Conclusions

FADD, TMEM16A, and PPFIA1 gene expressions as a whole were associated with disease-free survival in breast cancer.

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Alberts B, Wilson JH, Hunt T: Molecular Biology of the Cell. 2008, New York: Garland Science, 5

Peters G, Fantl V, Smith R, Brookes S, Dickson C: Chromosome 11q13 markers and D-type cyclins in breast cancer. Breast Cancer Res Treat. 1995, 33: 125-135. 10.1007/BF00682720.CrossRefPubMed

Karlseder J, Zeillinger R, Schneeberger C, Czerwenka K, Speiser P, Kubista E, Birnbaum D, Gaudray P, Theillet C: Patterns of DNA amplification at band q13 of chromosome 11 in human breast cancer. Genes Chromosomes Cancer. 1994, 9: 42-48. 10.1002/gcc.2870090108.CrossRefPubMed

Gaffey MJ, Frierson HF, Williams ME: Chromosome 11q13, c-erbB-2, and c-myc amplification in invasive breast carcinoma: clinicopathologic correlations. Mod Pathol. 1993, 6: 654-659.PubMed

Shi ZZ, Jiang YY, Hao JJ, Zhang Y, Zhang TT, Shang L, Liu SG, Shi F, Wang MR: Identification of putative target genes for amplification within 11q13.2 and 3q27.1 in esophageal squamous cell carcinoma. Clin Transl Oncol. 2013, Epub ahead of print

Meredith SD, Levine PA, Burns JA, Gaffey MJ, Boyd JC, Weiss LM, Erickson NL, Williams ME: Chromosome 11q13 amplification in head and neck squamous cell carcinoma. Association with poor prognosis. Arch Otolaryngol Head Neck Surg. 1995, 121: 790-794. 10.1001/archotol.1995.01890070076016.CrossRefPubMed

Williams ME, Gaffey MJ, Weiss LM, Wilczynski SP, Schuuring E, Levine PA: Chromosome 11Q13 amplification in head and neck squamous cell carcinoma. Arch Otolaryngol Head Neck Surg. 1993, 119: 1238-1243. 10.1001/archotol.1993.01880230084013.CrossRefPubMed

Jarmuz-Szymczak M, Pelinska K, Kostrzewska-Poczekaj M, Bembnista E, Giefing M, Brauze D, Szaumkessel M, Marszalek A, Janiszewska J, Kiwerska K, Bartochowska A, Grenman R, Szyfter W, Szyfter K: Heterogeneity of 11q13 region rearrangements in laryngeal squamous cell carcinoma analyzed by microarray platforms and fluorescence in situ hybridization. Mol Biol Rep. 2013, 40: 4161-4171. 10.1007/s11033-013-2496-4.CrossRefPubMed

Blessmann M, Al-Dam A, Hanken H, Assaf AT, Riecke B, Klatt J, Simon R, Sauter G, Heiland M, Kluwe L, Grobe A: Amplification of the PPFIA1 gene region on 11q13 in oral squamous cell carcinomas (OSCC). J Craniomaxillofac Surg. 2013, 41: 845-849. 10.1016/j.jcms.2013.01.040.CrossRefPubMed

10.

Rosa-Rosa JM, Pita G, Gonzalez-Neira A, Milne RL, Fernandez V, Ruivenkamp C, van Asperen CJ, Devilee P, Benitez J: A 7 Mb region within 11q13 may contain a high penetrance gene for breast cancer. Breast Cancer Res Treat. 2009, 118: 151-159. 10.1007/s10549-009-0317-1.CrossRefPubMed

11.

Gibcus JH, Menkema L, Mastik MF, Hermsen MA, de Bock GH, van Velthuysen ML, Takes RP, Kok K, Alvarez Marcos CA, van der Laan BF, van den Brekel MW, Langendijk JA, Kluin PM, van der Wal JE, Schuuring E: Amplicon mapping and expression profiling identify the Fas-associated death domain gene as a new driver in the 11q13.3 amplicon in laryngeal/pharyngeal cancer. Clin Cancer Res. 2007, 13: 6257-6266. 10.1158/1078-0432.CCR-07-1247.CrossRefPubMed

12.

Schneider P, Thome M, Burns K, Bodmer JL, Hofmann K, Kataoka T, Holler N, Tschopp J: TRAIL receptors 1 (DR4) and 2 (DR5) signal FADD-dependent apoptosis and activate NF-kappaB. Immunity. 1997, 7: 831-836. 10.1016/S1074-7613(00)80401-X.CrossRefPubMed

13.

Chaudhary PM, Eby M, Jasmin A, Bookwalter A, Murray J, Hood L: Death receptor 5, a new member of the TNFR family, and DR4 induce FADD-dependent apoptosis and activate the NF-kappaB pathway. Immunity. 1997, 7: 821-830. 10.1016/S1074-7613(00)80400-8.CrossRefPubMed

14.

Pattje WJ, Melchers LJ, Slagter-Menkema L, Mastik MF, Schrijvers ML, Gibcus JH, Kluin PM, Hoegen-Chouvalova O, van der Laan BF, Roodenburg JL, van der Wal JE, Schuuring E, Langendijk JA: FADD expression is associated with regional and distant metastasis in squamous cell carcinoma of the head and neck. Histopathology. 2013, 63: 263-270. 10.1111/his.12174.CrossRefPubMed

15.

Prapinjumrune C, Morita K, Kuribayashi Y, Hanabata Y, Shi Q, Nakajima Y, Inazawa J, Omura K: DNA amplification and expression of FADD in oral squamous cell carcinoma. J Oral Pathol Med. 2009, 39: 525-532.PubMed

16.

Chen G, Bhojani MS, Heaford AC, Chang DC, Laxman B, Thomas DG, Griffin LB, Yu J, Coppola JM, Giordano TJ, Lin L, Adams D, Orringer MB, Ross BD, Beer DG, Rehemtulla A: Phosphorylated FADD induces NF-kappaB, perturbs cell cycle, and is associated with poor outcome in lung adenocarcinomas. Proc Natl Acad Sci U S A. 2005, 102: 12507-12512. 10.1073/pnas.0500397102.PubMedCentralCrossRefPubMed

17.

Katoh M: Comparative genomics on mammalian Fgf3-Fgf4 locus. Int J Oncol. 2005, 27: 281-285.PubMed

18.

Duvvuri U, Shiwarski DJ, Xiao D, Bertrand C, Huang X, Edinger RS, Rock JR, Harfe BD, Henson BJ, Kunzelmann K, Schreiber R, Seethala RS, Egloff AM, Chen X, Lui VW, Grandis JR, Gollin SM: TMEM16A induces MAPK and contributes directly to tumorigenesis and cancer progression. Cancer Res. 2012, 72: 3270-3281. 10.1158/0008-5472.CAN-12-0475-T.PubMedCentralCrossRefPubMed

19.

Caputo A, Caci E, Ferrera L, Pedemonte N, Barsanti C, Sondo E, Pfeffer U, Ravazzolo R, Zegarra-Moran O, Galietta LJ: TMEM16A, a membrane protein associated with calcium-dependent chloride channel activity. Science. 2008, 322: 590-594. 10.1126/science.1163518.CrossRefPubMed

20.

West RB, Corless CL, Chen X, Rubin BP, Subramanian S, Montgomery K, Zhu S, Ball CA, Nielsen TO, Patel R, Goldblum JR, Brown PO, Heinrich MC, van de Rijn M: The novel marker, DOG1, is expressed ubiquitously in gastrointestinal stromal tumors irrespective of KIT or PDGFRA mutation status. Am J Pathol. 2004, 165: 107-113. 10.1016/S0002-9440(10)63279-8.PubMedCentralCrossRefPubMed

21.

Stanich JE, Gibbons SJ, Eisenman ST, Bardsley MR, Rock JR, Harfe BD, Ordog T, Farrugia G: Ano1 as a regulator of proliferation. Am J Physiol Gastrointest Liver Physiol. 2011, 301: G1044-G1051. 10.1152/ajpgi.00196.2011.PubMedCentralCrossRefPubMed

22.

Britschgi A, Bill A, Brinkhaus H, Rothwell C, Clay I, Duss S, Rebhan M, Raman P, Guy CT, Wetzel K, George E, Popa MO, Lilley S, Choudhury H, Gosling M, Wang L, Fitzgerald S, Borawski J, Baffoe J, Labow M, Gaither LA, Bentires-Alj M: Calcium-activated chloride channel ANO1 promotes breast cancer progression by activating EGFR and CAMK signaling. Proc Natl Acad Sci U S A. 2013, 110: E1026-E1034. 10.1073/pnas.1217072110.PubMedCentralCrossRefPubMed

23.

Astro V, Asperti C, Cangi MG, Doglioni C, de Curtis I: Liprin-alpha1 regulates breast cancer cell invasion by affecting cell motility, invadopodia and extracellular matrix degradation. Oncogene. 2011, 30: 1841-1849. 10.1038/onc.2010.562.CrossRefPubMed

24.

Shen JC, Unoki M, Ythier D, Duperray A, Varticovski L, Kumamoto K, Pedeux R, Harris CC: Inhibitor of growth 4 suppresses cell spreading and cell migration by interacting with a novel binding partner, liprin alpha1. Cancer Res. 2007, 67: 2552-2558. 10.1158/0008-5472.CAN-06-3870.PubMedCentralCrossRefPubMed

25.

Tan KD, Zhu Y, Tan HK, Rajasegaran V, Aggarwal A, Wu J, Wu HY, Hwang J, Lim DT, Soo KC, Tan P: Amplification and overexpression of PPFIA1, a putative 11q13 invasion suppressor gene, in head and neck squamous cell carcinoma. Genes Chromosomes Cancer. 2008, 47: 353-362. 10.1002/gcc.20539.CrossRefPubMed

26.

Kim YI, Lee A, Lee BH, Kim SY: Prognostic significance of syndecan-1 expression in cervical cancers. J Gynecol Oncol. 2011, 22: 161-167. 10.3802/jgo.2011.22.3.161.PubMedCentralCrossRefPubMed

27.

Rasamny JJ, Allak A, Krook KA, Jo VY, Policarpio-Nicolas ML, Sumner HM, Moskaluk CA, Frierson HF, Jameson MJ: Cyclin D1 and FADD as biomarkers in head and neck squamous cell carcinoma. Otolaryngol Head Neck Surg. 2012, 146: 923-931. 10.1177/0194599811435052.CrossRefPubMed

28.

29.

Wang QR, Yao XQ, Wen G, Fan Q, Li YJ, Fu XQ, Li CK, Sun XG: Apigenin suppresses the growth of colorectal cancer xenografts via phosphorylation and up-regulated FADD expression. Oncol Lett. 2011, 2: 43-47.PubMedCentralCrossRefPubMed

30.

Tvrdik D, Skalova H, Dundr P, Povysil C, Velenska Z, Berkova A, Stanek L, Petruzelka L: Apoptosis - associated genes and their role in predicting responses to neoadjuvant breast cancer treatment. Med Sci Monit. 2012, 18: BR60-BR67.PubMedCentralCrossRefPubMed

31.

Dancau AM, Wuth L, Waschow M, Holst F, Krohn A, Choschzick M, Terracciano L, Politis S, Kurtz S, Lebeau A, Friedrichs K, Wencke K, Monni O, Simon R: PPFIA1 and CCND1 are frequently coamplified in breast cancer. Genes Chromosomes Cancer. 2010, 49: 1-8. 10.1002/gcc.20713.CrossRefPubMed

32.

Sugahara K, Michikawa Y, Ishikawa K, Shoji Y, Iwakawa M, Shibahara T, Imai T: Combination effects of distinct cores in 11q13 amplification region on cervical lymph node metastasis of oral squamous cell carcinoma. Int J Oncol. 2011, 39: 761-769.PubMed

Title: Prognostic significance of TMEM16A, PPFIA1, and FADD expression in invasive ductal carcinoma of the breast
Authors: Eun Ji Choi
Jeong A Yun
Sahrish Jabeen
Eun Kyoung Jeon
Hye Sung Won
Yoon Ho Ko
Su Young Kim
Publication date: 01-12-2014
Publisher: BioMed Central
Published in: World Journal of Surgical Oncology / Issue 1/2014
Electronic ISSN: 1477-7819
DOI: https://doi.org/10.1186/1477-7819-12-137

At a glance: The ONWARDS insulin icodec trials

Springer Medicine

Prognostic significance of TMEM16A, PPFIA1, and FADD expression in invasive ductal carcinoma of the breast

Abstract

Background

Methods

Results

Conclusions

At a glance: The ONWARDS insulin icodec trials

Springer Medicine

Abstract

Background

Methods

Results

Conclusions

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