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Journal of Inflammation

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Open Access 01-12-2012 | Research

Anti-inflammation effects of hydrogen saline in LPS activated macrophages and carrageenan induced paw oedema

Authors: Zheng Xu, Jiangrui Zhou, Jianmei Cai, Zhen Zhu, Xuejun Sun, Chunlei Jiang

Published in: Journal of Inflammation | Issue 1/2012

Abstract

Background

Oxidative stress is thought to play an important role in the pathogenesis of inflammation. Recent studies have found that hydrogen gas has the effect of eliminating free radicals. Whether hydrogen saline (more convenient to be used than hydrogen gas) has the anti-inflammation effect or not is still unknown.

Methods

Carrageenan-induced paw oedema and LPS-activated macrophages are studied in this article. Injection of carrageenan into the foot of a mouse elicited an acute inflammatory response characterized by increase of foot volume and infiltration of neutrophils. While tumor necrosis factorα(TNF-α) secreted by activated macrophages was determined by ELISA and real-time PCR.

Results

All parameters of inflammation (foot volume, infiltration of neutrophils, amount of TNF-α and the level of TNF-α's mRNA) were attenuated by the hydrogen saline treatment.

Conclusion

As a more convenient way than inhaling H₂, hydrogen saline exhibits a protective effect against inflammation and it might provide a novel therapeutic approach for inflammatory diseases.

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Cuzzocrea Salvatore, Mazzon Emanuela, Dugo Laura, Serraino Ivana, Ciccolo Antonio, Centorrino Tommaso, De Sarro Angela, Achille Caputi: Protective effects of n-acetylcysteine on lung injury and red blood cell modification induced by carrageenan in the rat. The FASEB Journal. 2001, 15: 1187-200.CrossRefPubMed

Salvemini D, Wang ZQ, Bourdon DM, Stern MK, Currie MG, Manning PT: Evidence of peroxynitrite involvement in the carrageenan-induced rat paw edema. Eur J Pharmacol. 1996, 303: 217-20.CrossRefPubMed

Cuzzocrea S, Zingarelli B, Costantino G, Szabó A, Salzman AL, Caputi AP, Szabó C: Beneficial effects of 3-aminobenzamide, an inhibitor of poly(ADP-ribose)synthetase in a rat model of splanchnic artery occlusion and reperfusion. Br JPharmacol. 1997, 121: 1065-74.CrossRef

Tracey WR, Nakane M, Kuk J, Budzik G, Klinghofer V, Harris R, Carter G: The nitric oxide synthase inhibitor, L-NG-monomethylarginine, reduces carrageenan-induced pleurisy in the rat. J Pharmacol Exp Ther. 1995, 273: 1295-9.PubMed

Wei XQ, Charles IG, Smith A, Ure J, Feng GJ, Huang FP, Xu D, Muller W, Moncada S, Liew FY: Altered immune responses in mice lacking inducible nitric oxide synthase. Nature (Lond). 1995, 375: 408-11.CrossRef

Salvemini D, Wang ZQ, Wyatt P, Bourdon DM, Marino MH, Manning PT, Currie MG: Nitric oxide: A key mediator in the early and late phase of carrageenan-induced rat paw inflammation. Br J Pharmacol. 1996, 118: 829-38.PubMedCentralCrossRefPubMed

Miller MJ, Thompson JH, Zhang XJ, Sadowska-Krowicka H, Kakkis JL, Munshi UK, Sandoval M, Rossi JL, Eloby-Childress S, Beckmann JS: Role of inducible nitric oxide synthase expression and peroxynitrite formation in guinea pig ileitis. Gastroenterology. 1995, 109: 1475-83.CrossRefPubMed

Chamulitat W, Skrepnik NV, Spitzer JJ: Endotoxin-induced oxidative stress in the rat small intestine: Role of nitric oxide. Shock. 1996, 5: 217-22.CrossRef

Kaur H, Halliwell B: Evidence for nitric oxide-mediated oxidative damage in chronic inflammation: Nitrotyrosine in serum and synovial fluid from rheumatoid patients. FEBS Lett. 1994, 350: 9-12.CrossRefPubMed

10.

Rachmilewitz D, Stamler JS, Karmeli F, Mullins ME, Singel DJ, Loscalzo J, Xavier RJ, Podolsky DK: Peroxynitrite-induced rat colitis: A new model of colonic inflammation. Gastroenterology. 1993, 105: 1681-8.PubMed

11.

Sheu SS, Nauduri D, Anders MW: Targeting antioxidants to mitochondria: a new therapeutic direction. Biochim Biophys Acta. 2006, 1762: 256-65.CrossRefPubMed

12.

Ohsawa Ikuroh, Ishikawa Masahiro, Takahashi Kumiko, Watanabe Megumi, Nishimaki Kiyomi, Yamagata Kumi, Katsura Ken-ichiro, Katayama Yasuo, Asoh Sadamitsu, Ohta Shigeo: Hydrogen acts as a therapeutic antioxidant by selectively reducing cytotoxic oxygen radicals. Nature Medicine. 2007, 13: 688-94.CrossRefPubMed

13.

Kajiya M, Sato K, Silva MJ, Ouhara K, Do PM, Shanmugam KT, Kawai T: Hydrogen from intestinal bacteria is protective for Concanavalin A-induced hepatitis. Biochem Biophys Res Commun. 2009, 386 (2): 316-21.CrossRefPubMed

14.

Kajiya M, Silva MJ, Sato K, Ouhara K, Kawai T: Hydrogen mediates suppression of colon inflammation induced by dextran sodium sulfate. Biochem Biophys Res Commun. 2009, 386 (1): 11-5. Aug 14;386(1):11-5. 2009CrossRefPubMed

15.

Yanagihara T, Arai K, Miyamae K, Sato B, Shudo T, Yamada M, et al.: Electrolyzed hydrogen saturated water for drinking use elicits an antioxidative effect: a feeding test with rats. Biosci Biotechnol Biochem. 2005, 69: 1985-7.CrossRefPubMed

16.

Myoung Shin Eun, Yu Zhou Hong, Yu Guo Lian, Ah Kim Jeong, Ho Lee Seung, Merfort d Irmgard, Sik Kang Sam, Sung Kim Hak, Kim Sanghee, Shik Kim Yeong: Anti-inflammation effects of glycyrol isolated from Glycyrrhiza uralensis (Leguminosae) in LPS-induced RAW264.7 macrophages. Int Immunopharmacol. 2008, 10: 1016-25.

17.

Mazzon E, Esposito E, Di Paola R, Muià C, Crisafulli C, Genovese T, Caminiti R, Meli R, Bramanti P, Cuzzocrea S: Effect of tumour necrosis factor-alpha receptor 1 genetic deletion on carrageenan-induced acute inflammation: a comparison with etanercept. Clin Exp Immunol. 2008, 153: 136-49.PubMedCentralCrossRefPubMed

18.

Cai Jianmei, Kang Zhimin, Liu Kan, Liu WenWu, Li RunPing, H John Zhang, Luo Xu, Sun Xuejun: Neuroprotective Effects of Hydrogen Saline in Neonatal Hypoxia-ischemia Rat Model. Brain Research. 2008, doi:10.1016

19.

Morris CJ: Carrageenan-induced paw edema in the rat and mouse. Methods Mol Biol. 2003, 225: 115-221.PubMed

20.

Recio MC, Ginet RM, Uribun L, Manez S, Cerda M, De La Fuente JR, Rios JL: In vivo activity of pseudoguaianolide sesquiterpene lactones in acute andchronic inflammation. Life Sciences. 2000, 66: 2509-18.CrossRefPubMed

21.

Kim GY, Choi GS, Lee SH, Park YM: Acidicpolysaccharide isolated from Phellinus linteus enhances through the up-regulation of nitric oxide and tumor necrosis factor-afrom peritoneal macrophages. J Ethnopharmacol. 2004, 95: 69-76.CrossRefPubMed

22.

Park Pil-hoon, R McMullen Megan, Huang Honglian, Thakur Varsha, E Nagy Laura: Short-term Treatment of RAW264.7 Macrophages with Adiponectin Increases Tumor Necrosis Factor- a (TNF- a) Expression via ERK1/2 Activation and Egr-1 Expression. The Journal Of Biological Chemistry. 2007, 282: 21695-703.PubMedCentralCrossRefPubMed

23.

Raadan ODBILEG, Satoru KONNAI, Tatsufumi USUI, Kazuhiko OHASHI and Misao ONUMA: Quantification of Llama Inflammatory Cytokine mRNAs by Real-Time RT-PCR. J Vet Med Sci. 2005, 67: 195-8.CrossRefPubMed

24.

Simpson DA, Feeney S, Boyle C, Stitt AW: Retinal VEGF mRNA measured by SYBR green I fluorescence: A versatile approach to quantitative PCR. Molecular Vision. 2000, 6: 178-83.PubMed

25.

Choy CS, Hu CM, Chiu WT, Lam CS, Ting Y, Tsai SH, Wang TC: Suppression of lipopolysaccharide-induced of inducible nitric oxide synthase and cyclooxygenase-2 by Sanguis Draconis, a dragon's blood resin, in RAW 264.7 cells. J Ethnopharmacol. 2008, 115: 455-62.CrossRefPubMed

26.

Ohsawa Ikuroh, Nishimaki Kiyomi, Yamagata Kumi, Ishikawa Masahiro, Ohta Shigeo: Consumption of hydrogen water prevents atherosclerosis in apolipoprotein E knockout mice. Biochemical and Biophysical Research Communications. 2008, 377: 1195-1198.CrossRefPubMed

27.

Kajiyama Sizuo, Hasegawa Goji, Asano Mai, Hosoda Hiroko, Fukui Michiaki, Nakamura Naoto, Kitawaki Jo, Imai Saeko, Nakano Koji, Ohta Mitsuhiro, Adachi Tetsuo, Obayashi Hiroshi, Yoshikawa Toshikazu: Supplementation of hydrogen-rich water improves lipid and glucose metabolism in patients with type 2 diabetes or impaired glucose tolerance. Nutrition Research. 2008, 28: 137-143.CrossRefPubMed

28.

Fujita Kyota, Seike Toshihiro, Yutsudo Noriko, Ohno Mizuki, Yamada Hidetaka, Yamaguchi Hiroo, Sakumi Kunihiko, Yamakawa Yukiko, A Kido Mizuho, Takaki Atsushi, Katafuchi Toshihiko, Tanaka Yoshinori, Nakabeppu Yusaku, Noda Mami: Hydrogen in Drinking Water Reduces Dopaminergic Neuronal Loss in the 1-methyl-4-phenyl-1,2,3,6- tetrahydropyridine Mouse Model of Parkinson's Disease. PLOS ONE. 2009, 4 (9): e7247-PubMedCentralCrossRefPubMed

29.

Lallouette P, Richou R, Schwartz A, Richou H: Anti-inflamma-tory activity of inflammatory substances. General action of carrageenan on various acute inflammation models. C R Acad Sci Hebd Seances Acad Sci D. 1970, 270: 876-8.PubMed

30.

Park H, Kim YH, Chang HW, Kim HP: Anti-inflammation activity of the synthetic C-C biflavonoids. J Pharm Pharmacol. 2006, 58: 1661-7.CrossRefPubMed

31.

Park HJ, Kim IT, Won JH, Jeong SH, Park EY, Nam JH: Anti-inflammatory activities of ent-16alphaH,17-hydroxy-kauran-19-oic acid isolated from the roots of Siegesbeckia pubescens are due to the inhibition of iNOS and COX-2 expression in RAW 264.7 macrophages via NF-kappaB inactivation. Eur J Pharmacol. 2007, 558: 185-93.CrossRefPubMed

32.

Malleo G, Mazzon E, Genovese T, Di Paola R, Muià C, Centorrino T, Siriwardena AK, Cuzzocrea S: Etanercept attenuates the development of cerulein-induced acute pancreatitis in mice: a comparison with TNF-alpha genetic deletion. Shock. 2007, 27: 542-51.CrossRefPubMed

Title: Anti-inflammation effects of hydrogen saline in LPS activated macrophages and carrageenan induced paw oedema
Authors: Zheng Xu
Jiangrui Zhou
Jianmei Cai
Zhen Zhu
Xuejun Sun
Chunlei Jiang
Publication date: 01-12-2012
Publisher: BioMed Central
Published in: Journal of Inflammation / Issue 1/2012
Electronic ISSN: 1476-9255
DOI: https://doi.org/10.1186/1476-9255-9-2

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Anti-inflammation effects of hydrogen saline in LPS activated macrophages and carrageenan induced paw oedema

Abstract

Background

Methods

Results

Conclusion

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Abstract

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Conclusion

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