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Open Access 01-12-2014 | Research

Decreased percentage of CD4⁺Foxp3⁺TGF-β⁺ and increased percentage of CD4⁺IL-17⁺ cells in bronchoalveolar lavage of asthmatics

Authors: Adam Barczyk, Wladyslaw Pierzchala, Gaetano Caramori, Ryszard Wiaderkiewicz, Marcin Kaminski, Peter J Barnes, Ian M Adcock

Published in: Journal of Inflammation | Issue 1/2014

Abstract

Background

Asthma is a chronic inflammatory disorder of the airways with the proven role of Th2 cells in its pathogenesis. The role and characteristic of different subsets of CD4⁺ cells is much less known.

Aim

The aim of the study was to analyze the incidence of different subsets of CD4⁺ T cells, in particular different subsets of CD4⁺ cells with the co-expression of different cytokines.

Methods

Twenty five stable asthmatic and twelve age-matched control subjects were recruited to the study. Bronchoscopy and bronchoalveolar lavage (BAL) were performed in all study subjects. CD4⁺ T cells were isolated from BAL fluid by positive magnetic selection. After stimulation simultaneous expression of TGF-β, FoxP3, CD25, IFN-γ, IL-4, TNF-α (set 1); IL-10, FoxP3, CD25, IFN-γ, IL-4, MIP-1β (set 2); IL-17A, IL-8, IFN-γ, IL-4, MIP-1β (set 3) were measured by flow cytometry.

Results

The percentage of CD4⁺ cells co-expressing Foxp3 and TGF-β (CD4⁺Foxp3⁺TGF-β⁺ cells) was significantly lower (P = 0.03), whereas the percentage of CD4⁺IL-17⁺ cells (P = 0.008), CD4⁺IL-17⁺ IFN-γ⁺ cells (P = 0.047) and CD4⁺IL-4⁺ cells (P = 0.01) were significantly increased in asthmatics compared with that seen in healthy subjects. A significantly higher percentage of CD4⁺Foxp3⁺ cells from asthma patients expressed IFN-γ (P = 0.01), IL-4 (P = 0.004) and CD25 (P = 0.04), whereas the percentage of CD4⁺IL-10⁺ cells expressing Foxp3 was significantly decreased in asthmatics (P = 0.03). FEV₁% predicted correlated negatively with the percentage of CD4⁺IL-17⁺ cells (r = -0.33; P = 0.046) and positively with CD4⁺Foxp3⁺TGF-β⁺ cells (r = 0.43; P = 0.01).

Conclusions

Our results suggest that in the airways of chronic asthma patients there is an imbalance between increased numbers of CD4⁺IL-17⁺ cells and Th2 cells and decreased number of CD4⁺Foxp3⁺TGF-β⁺.

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Title: Decreased percentage of CD4+Foxp3+TGF-β+ and increased percentage of CD4+IL-17+ cells in bronchoalveolar lavage of asthmatics
Authors: Adam Barczyk
Wladyslaw Pierzchala
Gaetano Caramori
Ryszard Wiaderkiewicz
Marcin Kaminski
Peter J Barnes
Ian M Adcock
Publication date: 01-12-2014
Publisher: BioMed Central
Published in: Journal of Inflammation / Issue 1/2014
Electronic ISSN: 1476-9255
DOI: https://doi.org/10.1186/1476-9255-11-22

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Decreased percentage of CD4⁺Foxp3⁺TGF-β⁺ and increased percentage of CD4⁺IL-17⁺ cells in bronchoalveolar lavage of asthmatics

Abstract

Background

Aim

Methods

Results

Conclusions

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