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Open Access 01-12-2013 | Short Report

Activation of conventional protein kinase C (PKC) is critical in the generation of human neutrophil extracellular traps

Authors: Robert D Gray, Christopher D Lucas, Annie MacKellar, Feng Li, Katia Hiersemenzel, Chris Haslett, Donald J Davidson, Adriano G Rossi

Published in: Journal of Inflammation | Issue 1/2013

Abstract

Background

Activation of NADPH oxidase is required for neutrophil extracellular trap (NET) formation. Protein kinase C (PKC) is an upstream mediator of NADPH oxidase activation and thus likely to have a role in NET formation.

Methods

Pharmacological inhibitors were used to block PKC activity in neutrophils harvested from healthy donor blood.

Results

Pan PKC inhibition with Ro-31-8220 (p<0.001), conventional PKC inhibition with Go 6976 (p<0.001) and specific PKCβ inhibition with LY333531 (p<0.01) blocked NET formation in response to PMA. Inhibition of novel and atypical PKC had no effect. LY333531 blocked NET induction by the diacylglycerol analogue OAG (conventional PKC activator) (p<0.001).

Conclusions

Conventional PKCs have a prominent role in NET formation. Furthermore PKCβ is the major isoform implicated in NET formation.

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Title: Activation of conventional protein kinase C (PKC) is critical in the generation of human neutrophil extracellular traps
Authors: Robert D Gray
Christopher D Lucas
Annie MacKellar
Feng Li
Katia Hiersemenzel
Chris Haslett
Donald J Davidson
Adriano G Rossi
Publication date: 01-12-2013
Publisher: BioMed Central
Published in: Journal of Inflammation / Issue 1/2013
Electronic ISSN: 1476-9255
DOI: https://doi.org/10.1186/1476-9255-10-12

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Activation of conventional protein kinase C (PKC) is critical in the generation of human neutrophil extracellular traps

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Results

Conclusions

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