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Molecular Cancer

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Open Access 01-12-2013 | Research

Characterization and identification of PARM-1 as a new potential oncogene

Authors: Cyndia Charfi, Louis-Charles Levros Jr, Elsy Edouard, Eric Rassart

Published in: Molecular Cancer | Issue 1/2013

Abstract

Background

The Graffi murine retrovirus is a powerful tool to find leukemia associated oncogenes. Using DNA microarrays, we recently identified several genes specifically deregulated in T- and B-leukemias induced by this virus.

Results

In the present study, probsets associated with T-CD8⁺ leukemias were analyzed and we validated the expression profile of the Parm-1 gene. PARM-1 is a member of the mucin family. We showed that human PARM-1 is an intact secreted protein accumulating predominantly, such as murine PARM-1, at the Golgi and in the early and late endosomes. PARM-1 colocalization with α-tubulin suggests that its trafficking within the cell involves the microtubule cytoskeleton. Also, the protein co-localizes with caveolin-1 which probably mediates its internalization. Transient transfection of both mouse and human Parm-1 cDNAs conferred anchorage- and serum-independent growth and enhanced cell proliferation. Moreover, deletion mutants of human PARM-1 without either extracellular or cytoplasmic portions seem to retain the ability to induce anchorage-independent growth of NIH/3T3 cells. In addition, PARM-1 increases ERK1/2, but more importantly AKT and STAT3 phosphorylation.

Conclusions

Our results strongly suggest the oncogenic potential of PARM-1.

Available only for authorised users

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Title: Characterization and identification of PARM-1 as a new potential oncogene
Authors: Cyndia Charfi
Louis-Charles Levros Jr
Elsy Edouard
Eric Rassart
Publication date: 01-12-2013
Publisher: BioMed Central
Published in: Molecular Cancer / Issue 1/2013
Electronic ISSN: 1476-4598
DOI: https://doi.org/10.1186/1476-4598-12-84

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Background

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