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Malaria Journal

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Open Access 01-12-2008 | Research

Extensive dynamics of Plasmodium falciparum densities, stages and genotyping profiles

Authors: Anna Färnert, Marianne Lebbad, Lea Faraja, Ingegerd Rooth

Published in: Malaria Journal | Issue 1/2008

Abstract

Background

Individuals living in areas of high malaria transmission often have different Plasmodium falciparum clones detected in the peripheral blood over time. The aim of this study was to assess the dynamics of asymptomatic P. falciparum infections in a few hours intervals.

Methods

Capillary blood samples were collected 6-hourly during five days from asymptomatic children in a highly endemic area in Tanzania. Parasite densities and maturation stages were investigated by light microscopy. Types and number of clones were analysed by PCR based genotyping of the polymorphic merozoite surface proteins 1 and 2 genes. Results: Parasite densities and maturation stages fluctuated 48-hourly with a gradual shift into more mature forms. Various genotyping patterns were observed in repeated samples over five days with only few samples with identical profiles. Up to six alleles differed in samples collected six hours apart in the same individual.

Conclusion

This detailed assessment highlights the extensive within-host dynamics of P. falciparum populations and the limitations of single blood samples to determine parasite densities, stages and genotyping profiles in a malaria infected individual.

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Title: Extensive dynamics of Plasmodium falciparum densities, stages and genotyping profiles
Authors: Anna Färnert
Marianne Lebbad
Lea Faraja
Ingegerd Rooth
Publication date: 01-12-2008
Publisher: BioMed Central
Published in: Malaria Journal / Issue 1/2008
Electronic ISSN: 1475-2875
DOI: https://doi.org/10.1186/1475-2875-7-241

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