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Malaria Journal
Published in: Malaria Journal 1/2007

Open Access 01-12-2007 | Research

Genetic population structure of Anopheles gambiae in Equatorial Guinea

Authors: Marta Moreno, Patricia Salgueiro, José Luis Vicente, Jorge Cano, Pedro J Berzosa, Aida de Lucio, Frederic Simard, Adalgisa Caccone, Virgilio E Do Rosario, João Pinto, Agustín Benito

Published in: Malaria Journal | Issue 1/2007

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Abstract

Background

Patterns of genetic structure among mosquito vector populations in islands have received particular attention as these are considered potentially suitable sites for experimental trials on transgenic-based malaria control strategies. In this study, levels of genetic differentiation have been estimated between populations of Anopheles gambiae s.s. from the islands of Bioko and Annobón, and from continental Equatorial Guinea (EG) and Gabon.

Methods

Genotyping of 11 microsatellite loci located in chromosome 3 was performed in three island samples (two in Bioko and one in Annobón) and three mainland samples (two in EG and one in Gabon). Four samples belonged to the M molecular form and two to the S-form. Microsatellite data was used to estimate genetic diversity parameters, perform demographic equilibrium tests and analyse population differentiation.

Results

High levels of genetic differentiation were found between the more geographically remote island of Annobón and the continent, contrasting with the shallow differentiation between Bioko island, closest to mainland, and continental localities. In Bioko, differentiation between M and S forms was higher than that observed between island and mainland samples of the same molecular form.

Conclusion

The observed patterns of population structure seem to be governed by the presence of both physical (the ocean) and biological (the M-S form discontinuity) barriers to gene flow. The significant degree of genetic isolation between M and S forms detected by microsatellite loci located outside the "genomic islands" of speciation identified in A. gambiae s.s. further supports the hypothesis of on-going incipient speciation within this species. The implications of these findings regarding vector control strategies are discussed.
Appendix
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Literature
1.
WHO/UNICEF/RBM: World Malaria Report 2005. WHO/HTM/MAL/2005.1102. 2005, World Health Organization, Geneva
2.
3.
Brey PT: Anopheles gambiae genome: perspectives for malaria control. Mol Cells. 2003, 15: 133-138.PubMed
4.
Lehmann T, Licht M, Elissa N, Maega BT, Chimumbwa JM, Watsenga FT, Wondji CS, Simard F, Hawley WA: Population Structure of Anopheles gambiae in Africa. J Hered. 2003, 94: 133-147. 10.1093/jhered/esg024.CrossRefPubMed
5.
Coluzzi M, Petrarca V, Di Deco M: Chromosomal inversion intergradation in incipient speciation in Anopheles gambiae. Boll Zool. 1985, 52: 45-63.CrossRef
6.
Della Torre A, Costantini C, Besansky NJ, Caccone A, Petrarca V, Powell JR, Coluzzi M: Speciation within Anopheles gambiae – the glass is half full. Science. 2002, 298: 115-117. 10.1126/science.1078170.CrossRefPubMed
7.
Touré YT, Petrarca V, Traoré SF, Coulibaly A, Maiga HM, Sankare O, Sow M, Di Deco MA, Coluzzi M: The distribution and inversion polymorphism of chromosomally recognized taxa of the Anopheles gambiae complex in Mali, West Africa. Parassitologia. 1998, 40: 477-511.PubMed
8.
Della Torre A, Akogbeto M, Sou-Yovo J, Favia G, Petrarca V, Coluzzi M: Molecular evidence of incipient speciation within Anopheles gambiae s.s. in West Africa. Insect Mol Biol. 2001, 10: 9-18. 10.1046/j.1365-2583.2001.00235.x.CrossRefPubMed
9.
Gentile G, Slotman M, Ketmaier V, Powell JR, Caccone A: Attempts to molecularly distinguish cryptic taxa in Anopheles gambiae s.s. Insect Mol Biol. 2001, 10: 25-32. 10.1046/j.1365-2583.2001.00237.x.CrossRefPubMed
10.
Diabate A, Dabire RK, Millogo N, Lehmann T: Evaluating the effect of postmating isolation between molecular forms of Anopheles gambiae (Diptera: Culicidae). J Med Entomol. 2007, 44: 60-64. 10.1603/0022-2585(2007)44[60:ETEOPI]2.0.CO;2.CrossRefPubMed
11.
Tripet F, Toure YT, Taylor CE, Norris DE, Dolo G, Lanzaro GC: DNA analysis of transferred sperm reveals significant levels of gene flow between molecular forms of Anopheles gambiae. Mol Ecol. 2001, 10: 1725-1732. 10.1046/j.0962-1083.2001.01301.x.CrossRefPubMed
12.
Fanello C, Petrarca V, della TA, Santolamazza F, Dolo G, Coulibaly M, Alloueche A, Curtis CF, Toure YT, Coluzzi M: The pyrethroid knock-down resistance gene in the Anopheles gambiae complex in Mali and further indication of incipient speciation within An. gambiae s.s. Insect Mol Biol. 2003, 12: 241-245. 10.1046/j.1365-2583.2003.00407.x.CrossRefPubMed
13.
Gentile G, Santolamazza F, Fanello C, Petrarca V, Caccone A, della Torre A: Variation in an intron sequence of the voltage-gated sodium channel gene correlates with genetic differentiation between Anopheles gambiae s.s. molecular forms. Insect Mol Biol. 2004, 13: 371-377. 10.1111/j.0962-1075.2004.00494.x.CrossRefPubMed
14.
Donnelly MJ, Simard F, Lehmann T: Evolutionary studies of malaria vectors. Trends Parasitol. 2002, 18: 75-80. 10.1016/S1471-4922(01)02198-5.CrossRefPubMed
15.
Krzywinski J, Besansky NJ: Molecular systematics of Anopheles : from subgenera to subpopulations. Annu Rev Entomol. 2003, 48: 111-139. 10.1146/annurev.ento.48.091801.112647.CrossRefPubMed
16.
Wondji C, Simard F, Fontenille D: Evidence for genetic differentiation between the molecular forms M and S within the Forest chromosomal form of Anopheles gambiae in an area of sympatry. Insect Mol Biol. 2002, 11: 11-19. 10.1046/j.0962-1075.2001.00306.x.CrossRefPubMed
17.
Lehmann T, Hawley WA, Kamau L, Fontenille D, Simard F, Collins FH: Genetic differentiation of Anopheles gambiae populations from East and west Africa: comparison of microsatellite and allozyme loci. Heredity. 1996, 77 (Pt 2): 192-200.CrossRefPubMed
18.
Pinto J, Donnelly MJ, Sousa CA, Gil V, Ferreira C, Elissa N, Do Rosario VE, Charlwood JD: Genetic structure of Anopheles gambiae (Diptera: Culicidae) in Sao Tome and Principe (West Africa): implications for malaria control. Mol Ecol. 2002, 11: 2183-2187. 10.1046/j.1365-294X.2002.01587.x.CrossRefPubMed
19.
Simard F, Fontenille D, Lehmann T, Girod R, Brutus L, Gopaul R, Dournon C, Collins FH: High amounts of genetic differentiation between populations of the malaria vector Anopheles arabiensis from West Africa and eastern outer islands. Am J Trop Med Hyg. 1999, 60: 1000-1009.PubMed
20.
Slotman MA, Mendez MM, Torre AD, Dolo G, Toure YT, Caccone A: Genetic differentiation between the BAMAKO and SAVANNA chromosomal forms of Anopheles gambiae as indicated by amplified fragment length polymorphism analysis. Am J Trop Med Hyg. 2006, 74: 641-648.PubMed
21.
Slotman MA, Tripet F, Cornel AJ, Meneses CR, Lee Y, Reimer LJ, Thiemann TC, Fondjo E, Fofana A, Traore SF, Lanzaro GC: Evidence for subdivision within the M molecular form of Anopheles gambiae. Mol Ecol. 2007, 16: 639-649. 10.1111/j.1365-294X.2006.03172.x.CrossRefPubMed
22.
Berzosa PJ, Cano J, Roche J, Rubio JM, Garcia L, Moyano E, Guerra A, Mateos JC, Petrarca V, Rosario VE, Benito A: Malaria vectors in Bioko Island (Equatorial Guinea): PCR determination of the members of Anopheles gambiae Giles complex (Diptera: Culicidae) and pyrethroid knockdown resistance (kdr) in An. gambiae sensu stricto. J Vector Ecol. 2002, 27: 102-106.PubMed
23.
Pardo G, Descalzo MA, Molina L, Custodio E, Lwanga M, Mangue C, Obono J, Nchama A, Roche J, Benito A, Cano J: Impact of different strategies to control Plasmodium infection and anaemia on the island of Bioko (Equatorial Guinea). Malar J. 2006, 5: 10-10.1186/1475-2875-5-10.PubMedCentralCrossRefPubMed
24.
Kayondo J, Mukwaya L, Stump A, Michel A, Coulibaly M, Besansky N, Collins FH: Genetic structure of Anopheles gambiae populations on islands in northwestern Lake Victoria, Uganda. Malar J. 2005, 4: 59-10.1186/1475-2875-4-59.PubMedCentralCrossRefPubMed
25.
Gillies MT, Coetzee MT: A Supplement to the Anophelinae of Africa south of the Sahara (Ethiopian zoogeographical region). S Afr Ins Med Res. 1987, 2: 55-
26.
Collins FH, Finnerty V, Petrarca V: Ribosomal DNA-probes differentiate five cryptic species in the Anopheles gambiae complex. Parassitologia. 1988, 30: 231-240.PubMed
27.
Scott JA, Brogdon WG, Collins FH: Identification of single specimens of the Anopheles gambiae complex by the polymerase chain reaction. Am J Trop Med Hyg. 1993, 49: 520-529.PubMed
28.
Favia G, Lanfracotti A, Spanos L, Siden K, Louis C: Molecular characterization of ribosomal DNA polymorphisms discriminating among chromosomal forms of Anopheles gambiae s.s. Insect Mol Biol. 2001, 10: 19-23. 10.1046/j.1365-2583.2001.00236.x.CrossRefPubMed
29.
Pinto J, Lynd A, Elissa N, Donnelly MJ, Costa C, Gentile G, Caccone A, do Rosario VE: Co-occurrence of East and West African kdr mutations suggests high levels of resistance to pyrethroid insecticides in Anopheles gambiae from Libreville, Gabon. Med Vet Entomol. 2006, 20: 27-32. 10.1111/j.1365-2915.2006.00611.x.CrossRefPubMed
30.
Zheng L, Benedict MQ, Cornel AJ, Collins FH, Kafatos FC: An integrated genetic map of the African human malaria vector mosquito, Anopheles gambiae. Genetics. 1996, 143: 941-952.PubMedCentralPubMed
31.
Lanzaro GC, Touré YT, Carnahan J, Zheng L, Dolo G, Traoré , Petrarca V, Vernick KD, Taylor CE: Complexities in the genetic structure of Anopheles gambiae populations in west Africa as revealed by microsatellite DNA analysis. Proc Natl Acad Sci USA. 1998, 95: 14260-14265. 10.1073/pnas.95.24.14260.PubMedCentralCrossRefPubMed
32.
Stump AD, Shoener JA, Costantini C, Sagnon N, Besansky NJ: Sex-linked differentiation between incipient species of Anopheles gambiae. Genetics. 2005, 169: 1509-1519. 10.1534/genetics.104.035303.PubMedCentralCrossRefPubMed
33.
Donnelly MJ, Cuamba N, Charlwood JD, Collins FH, Townson H: Population structure in the malaria vector, Anopheles arabiensis Patton, in East Africa. Heredity. 1999, 83 (Pt 4): 408-417. 10.1038/sj.hdy.6885930.CrossRefPubMed
34.
Nei M: Molecular Evolutionary Genetics. 1987, New York: Columbia University Press
35.
El Mousadik A, Petit R: High level of genetic differentiation for allelic richness among populations of the argan tree Argania spinosa L.) Skeels endemic to Morocco. Theoret Appl Genet. 1996, 92: 832-839. 10.1007/BF00221895.CrossRef
36.
Goudet J: FSTAT Version 1.2: A computer program to calculate F-statistics. J Hered. 1995, 86: 485-
37.
Raymond M, Rousset F: GENEPOP (Version 1.2): Population genetics software for exact tests and ecumenism. J Hered. 1995, 86: 248-249.
38.
Cornuet JM, Luikart G: Description and power analysis of two tests for detecting recent population bottlenecks from allele frequency data. Genetics. 1996, 144: 2001-2014.PubMedCentralPubMed
39.
Weir BS, Cockerham CC: Estimating F-statistics for the analysis of population structure. Evol. 1984, 38: 1358-1370. 10.2307/2408641.CrossRef
40.
Schneider S, Roessli D, Excoffier L: ARLEQUIN, Version 2 000: A software for population genetics data analysis. 2000
41.
Rousset F: Genetic differentiation and estimation of gene flow from F-statistics under isolation by distance. Genetics. 1997, 145: 1219-1228.PubMedCentralPubMed
42.
Piry S, Alapetite A, Cornuet JM, Paetkau D, Baudouin L, Estoup A: GENECLASS2: a software for genetic assignment and first-generation migrant detection. J Hered. 2004, 95: 536-539. 10.1093/jhered/esh074.CrossRefPubMed
43.
44.
Paetkau D, Slade R, Burden M, Estoup A: Genetic assignment methods for the direct, real-time estimation of migration rate: a simulation-based exploration of accuracy and power. Mol Ecol. 2004, 13: 55-65. 10.1046/j.1365-294X.2004.02008.x.CrossRefPubMed
45.
Pritchard JK, Stephens M, Donnelly P: Inference of population structure using multilocus genotype data. Genetics. 2000, 155: 945-959.PubMedCentralPubMed
46.
Evanno G, Regnaut S, Goudet J: Detecting the number of clusters of individuals using the software STRUCTURE: a simulation study. Mol Ecol. 2005, 14: 2611-2620. 10.1111/j.1365-294X.2005.02553.x.CrossRefPubMed
47.
Holm S: A simple sequentially rejective multiple test procedure. Scand J Statistics. 1979, 6: 65-70.
48.
Onyabe DY, Conn JE: Genetic differentiation of the malaria vector Anopheles gambiae across Nigeria suggests that selection limits gene flow. Heredity. 2001, 87: 647-658. 10.1046/j.1365-2540.2001.00957.x.CrossRefPubMed
49.
Yawson AE, Weetman D, Wilson MD, Donnelly MJ: Ecological zones rather than molecular forms predict genetic differentiation in the malaria vector Anopheles gambiae s.s. in Ghana. Genetics. 2007, 175: 751-761. 10.1534/genetics.106.065888.PubMedCentralCrossRefPubMed
50.
Gillespie RG, Roderick GK: Arthropods on islands: colonisation, speciation and conservation. Annu Rev Entomol. 2002, 47: 595-632. 10.1146/annurev.ento.47.091201.145244.CrossRefPubMed
51.
Donnelly MJ, Licht MC, Lehmann T: Evidence for recent population expansion in the evolutionary history of the malaria vectors Anopheles arabiensis and Anopheles gambiae. Mol Biol Evol. 2001, 18: 1353-1364.CrossRefPubMed
52.
Roche J, de Diego JA, Penin P, Santos M, del RJ: An epidemiological study of malaria in Bioko and Annobon islands (Equatorial Guinea). Ann Trop Med Parasitol. 1991, 85: 477-487.PubMed
53.
Lehmann T, Hawley WA, Grebert H, Collins FH: The effective population size of Anopheles gambiae in Kenya: implications for population structure. Mol Biol Evol. 1998, 15: 264-276.CrossRefPubMed
54.
Simard F, Lehmann T, Lemasson JJ, Diatta M, Fontenille D: Persistence of Anopheles arabiensis during the severe dry season conditions in Senegal: an indirect approach using microsatellite loci. Insect Mol Biol. 2000, 9: 467-479. 10.1046/j.1365-2583.2000.00210.x.CrossRefPubMed
55.
Reimer LJ, Tripet F, Slotman M, Spielman A, Fonjo E, Lanzaro GC: An unusual distribution of the kdr gene among populations of Anopheles gambiae on the island of Bioko, Equatorial Guinea. Insect Mol Biol. 2005, 14: 683-688. 10.1111/j.1365-2583.2005.00599.x.CrossRefPubMed
56.
Gentile G, della TA, Maegga B, Powell JR, Caccone A: Genetic differentiation in the African malaria vector, Anopheles gambiae s.s., and the problem of taxonomic status. Genetics. 2002, 161: 1561-1578.PubMedCentralPubMed
57.
Boulesteix M, Simard F, Antonio-Nkondjio C, wono-Ambene HP, Fontenille D, Biemont C: Insertion polymorphism of transposable elements and population structure of Anopheles gambiae M and S molecular forms in Cameroon. Mol Ecol. 2007, 16: 441-452. 10.1111/j.1365-294X.2006.03150.x.CrossRefPubMed
58.
Wang R, Zheng L, Touré YT, Danadekar T, Kafatos FC: When genetic distance matters: measuring genetic differentiation at microsatellite loci in whole genome scans of recent and incipient mosquito species. Proc Natl Acad Sci USA. 2001, 98: 10769-10774. 10.1073/pnas.191003598.PubMedCentralCrossRefPubMed
59.
60.
Stump AD, Fitzpatrick MC, Lobo NF, Traore S, Sagnon N, Costantini C, Collins FH, Besansky NJ: Centromere-proximal differentiation and speciation in Anopheles gambiae. Proc Natl Acad Sci USA. 2005, 102: 15930-15935. 10.1073/pnas.0508161102.PubMedCentralCrossRefPubMed
61.
Slotman MA, Reimer LJ, Thiemann T, Dolo G, Fondjo E, Lanzaro GC: Reduced recombination rate and genetic differentiation between the M and S forms of Anopheles gambiae s.s. Genetics. 2006, 174: 2081-2093. 10.1534/genetics.106.059949.PubMedCentralCrossRefPubMed
62.
Lounibos LP: Invasions by insect vectors of human disease. Annu Rev Entomol. 2002, 47: 233-266. 10.1146/annurev.ento.47.091201.145206.CrossRefPubMed
63.
Ayala D, Goff GL, Robert V, de JP, Takken W: Population structure of the malaria vector Anopheles funestus (Diptera: Culicidae) in Madagascar and Comoros. Acta Trop. 2006, 97: 292-300. 10.1016/j.actatropica.2005.12.002.CrossRefPubMed
Metadata
Title
Genetic population structure of Anopheles gambiae in Equatorial Guinea
Authors
Marta Moreno
Patricia Salgueiro
José Luis Vicente
Jorge Cano
Pedro J Berzosa
Aida de Lucio
Frederic Simard
Adalgisa Caccone
Virgilio E Do Rosario
João Pinto
Agustín Benito
Publication date
01-12-2007
Publisher
BioMed Central
Published in
Malaria Journal / Issue 1/2007
Electronic ISSN: 1475-2875
DOI
https://doi.org/10.1186/1475-2875-6-137

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