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Published in: Malaria Journal 1/2003

Open Access 01-12-2003 | Methodology

Comparison of Plasmodium falciparum transfection methods

Authors: Tina S Skinner-Adams, Paula M Lawrie, Paula L Hawthorne, Donald L Gardiner, Katharine R Trenholme

Published in: Malaria Journal | Issue 1/2003

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Abstract

The development of an electroporation based transfection method for Plasmodium falciparum has been very successful for the study of some genes but its efficiency remains very low. While alternative approaches have been documented, electroporation of infected red blood cells generally remains the method of choice for introducing DNA into P. falciparum. In this paper we compare four published transfection techniques in their ability to achieve stable transfections.
Literature
1.
go back to reference van Dijk MR, Waters AP, Janse CJ: Stable transfection of malaria parasite blood stages. Science. 1995, 268: 1358-62.CrossRefPubMed van Dijk MR, Waters AP, Janse CJ: Stable transfection of malaria parasite blood stages. Science. 1995, 268: 1358-62.CrossRefPubMed
2.
go back to reference Wu Y, Sifri CD, Lei HH, Su XZ, Wellems TE: Transfection of Plasmodium falciparum within human red blood cells. Proc Natl Acad Sci U S A. 1995, 92: 973-7.PubMedCentralCrossRefPubMed Wu Y, Sifri CD, Lei HH, Su XZ, Wellems TE: Transfection of Plasmodium falciparum within human red blood cells. Proc Natl Acad Sci U S A. 1995, 92: 973-7.PubMedCentralCrossRefPubMed
3.
go back to reference Wu Y, Kirkman LA, Wellems TE: Transformation of Plasmodium falciparum malaria parasites by homologous integration of plasmids that confer resistance to pyrimethamine. Proc Natl Acad Sci U S A. 1996, 93: 1130-4. 10.1073/pnas.93.3.1130.PubMedCentralCrossRefPubMed Wu Y, Kirkman LA, Wellems TE: Transformation of Plasmodium falciparum malaria parasites by homologous integration of plasmids that confer resistance to pyrimethamine. Proc Natl Acad Sci U S A. 1996, 93: 1130-4. 10.1073/pnas.93.3.1130.PubMedCentralCrossRefPubMed
4.
go back to reference Crabb BS, Cowman AF: Characterization of promoters and stable transfection by homologous and nonhomologous recombination in Plasmodium falciparum. Proc Natl Acad Sci U S A. 1996, 93: 7289-94. 10.1073/pnas.93.14.7289.PubMedCentralCrossRefPubMed Crabb BS, Cowman AF: Characterization of promoters and stable transfection by homologous and nonhomologous recombination in Plasmodium falciparum. Proc Natl Acad Sci U S A. 1996, 93: 7289-94. 10.1073/pnas.93.14.7289.PubMedCentralCrossRefPubMed
5.
go back to reference Crabb BS, Triglia T, Waterkeyn JG, Cowman AF: Stable transgene expression in Plasmodium falciparum. Mol Biochem Parasitol. 1997, 90: 131-44. 10.1016/S0166-6851(97)00143-6.CrossRefPubMed Crabb BS, Triglia T, Waterkeyn JG, Cowman AF: Stable transgene expression in Plasmodium falciparum. Mol Biochem Parasitol. 1997, 90: 131-44. 10.1016/S0166-6851(97)00143-6.CrossRefPubMed
6.
go back to reference Gardiner DL, Holt DC, Thomas EA, Kemp DJ, Trenholme KR: Inhibition of Plasmodium falciparum clag9 gene function by antisense RNA. Mol Biochem Parasitol. 2000, 110: 33-41. 10.1016/S0166-6851(00)00254-1.CrossRefPubMed Gardiner DL, Holt DC, Thomas EA, Kemp DJ, Trenholme KR: Inhibition of Plasmodium falciparum clag9 gene function by antisense RNA. Mol Biochem Parasitol. 2000, 110: 33-41. 10.1016/S0166-6851(00)00254-1.CrossRefPubMed
7.
go back to reference Waller RF, Reed MB, Cowman AF, McFadden GI: Protein trafficking to the plastid of Plasmodium falciparum is via the secretory pathway. EMBO J. 2000, 19: 1794-802. 10.1093/emboj/19.8.1794.PubMedCentralCrossRefPubMed Waller RF, Reed MB, Cowman AF, McFadden GI: Protein trafficking to the plastid of Plasmodium falciparum is via the secretory pathway. EMBO J. 2000, 19: 1794-802. 10.1093/emboj/19.8.1794.PubMedCentralCrossRefPubMed
8.
go back to reference de Koning-Ward TF, Waters AP, Crabb BS: Puromycin-N-acetyltransferase as a selectable marker for use in Plasmodium falciparum. Mol Biochem Parasitol. 2001, 117: 155-60. 10.1016/S0166-6851(01)00344-9.CrossRefPubMed de Koning-Ward TF, Waters AP, Crabb BS: Puromycin-N-acetyltransferase as a selectable marker for use in Plasmodium falciparum. Mol Biochem Parasitol. 2001, 117: 155-60. 10.1016/S0166-6851(01)00344-9.CrossRefPubMed
9.
go back to reference Gardiner DL, Skinner Adams TS, Spielmann T, Trenholme KR: Malaria transfection and transfection vectors. Trends in Parasitol. 2003, Gardiner DL, Skinner Adams TS, Spielmann T, Trenholme KR: Malaria transfection and transfection vectors. Trends in Parasitol. 2003,
10.
go back to reference Deitsch K, Driskill C, Wellems T: Transformation of malaria parasites by the spontaneous uptake and expression of DNA from human erythrocytes. Nucleic Acids Res. 2001, 29: 850-3. 10.1093/nar/29.3.850.PubMedCentralCrossRefPubMed Deitsch K, Driskill C, Wellems T: Transformation of malaria parasites by the spontaneous uptake and expression of DNA from human erythrocytes. Nucleic Acids Res. 2001, 29: 850-3. 10.1093/nar/29.3.850.PubMedCentralCrossRefPubMed
11.
go back to reference Mamoun CB, Truong R, Gluzman I, Akopyants NS, Oksman A, Goldberg DE: Transfer of genes into Plasmodium falciparum by polyamidoamine dendrimers. Mol Biochem Parasitol. 1999, 103: 117-21. 10.1016/S0166-6851(99)00116-4.CrossRefPubMed Mamoun CB, Truong R, Gluzman I, Akopyants NS, Oksman A, Goldberg DE: Transfer of genes into Plasmodium falciparum by polyamidoamine dendrimers. Mol Biochem Parasitol. 1999, 103: 117-21. 10.1016/S0166-6851(99)00116-4.CrossRefPubMed
12.
go back to reference Fidock DA, Wellems TE: Transformation with human dihydrofolate reductase renders malaria parasites insensitive to WR99210 but does not affect the intrinsic activity of proguanil. Proc Natl Acad Sci U S A. 1997, 94: 10931-6. 10.1073/pnas.94.20.10931.PubMedCentralCrossRefPubMed Fidock DA, Wellems TE: Transformation with human dihydrofolate reductase renders malaria parasites insensitive to WR99210 but does not affect the intrinsic activity of proguanil. Proc Natl Acad Sci U S A. 1997, 94: 10931-6. 10.1073/pnas.94.20.10931.PubMedCentralCrossRefPubMed
13.
14.
go back to reference Reed MB, Saliba KJ, Caruana SR, Kirk K, Cowman AF: Pgh1 modulates sensitivity and resistance to multiple antimalarials in Plasmodium falciparum. Nature. 2000, 403: 906-09. 10.1038/35002615.CrossRefPubMed Reed MB, Saliba KJ, Caruana SR, Kirk K, Cowman AF: Pgh1 modulates sensitivity and resistance to multiple antimalarials in Plasmodium falciparum. Nature. 2000, 403: 906-09. 10.1038/35002615.CrossRefPubMed
Metadata
Title
Comparison of Plasmodium falciparum transfection methods
Authors
Tina S Skinner-Adams
Paula M Lawrie
Paula L Hawthorne
Donald L Gardiner
Katharine R Trenholme
Publication date
01-12-2003
Publisher
BioMed Central
Published in
Malaria Journal / Issue 1/2003
Electronic ISSN: 1475-2875
DOI
https://doi.org/10.1186/1475-2875-2-19

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