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Cancer Cell International
Published in: Cancer Cell International 1/2013

Open Access 01-12-2013 | Primary research

Silencing SATB1 with siRNA inhibits the proliferation and invasion of small cell lung cancer cells

Authors: Bo Huang, Hongli Zhou, Xiaodong Wang, Zhiliang Liu

Published in: Cancer Cell International | Issue 1/2013

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Abstract

Background

Small cell lung cancer (SCLC) is a special kind of lung cancers, lymph or blood metastasis of SCLC usually occurs in early stage. Studies in breast and colon cancer showed over expression of SATB1 could promote tumor cell growth and inhibit apoptosis. Therefore, we studied the expression of SATB1 in SCLC.

Methods

The level of SATB1 was analyzed in SCLC tissues, metastatic lymphoid nodes and adjacent normal lung tissues by immunohistochemistry. Meanwhile, small interfering SATB1-targeting RNA was constructed and transfected into human SCLC cell line NCI-H446 to evaluate the effects of SATB1-siRNA on cell proliferation, invasion and apoptosis.

Result

SATB1 protein was overexpressed in SCLC tissues and metastasis lymphoid nodes compared with adjacent normal lung tissues. Compared with control group, SATB1-siRNA inhibits the proliferation and invasion of SCLC cells and induces SCLC cells apoptosis statistically (P<0.05) in vitro.

Conclusion

Our results suggest that SATB1 plays an important role in the metastasis of human SCLC cell.
Appendix
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Metadata
Title
Silencing SATB1 with siRNA inhibits the proliferation and invasion of small cell lung cancer cells
Authors
Bo Huang
Hongli Zhou
Xiaodong Wang
Zhiliang Liu
Publication date
01-12-2013
Publisher
BioMed Central
Published in
Cancer Cell International / Issue 1/2013
Electronic ISSN: 1475-2867
DOI
https://doi.org/10.1186/1475-2867-13-8

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