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International Journal of Colorectal Disease

Published in:

01-02-2012 | Original Article

Correlation of SATB1 overexpression with the progression of human rectal cancer

Authors: Wen-Jian Meng, Hui Yan, Bin Zhou, Wei Zhang, Xiang-Heng Kong, Rong Wang, Lan Zhan, Yuan Li, Zong-Guang Zhou, Xiao-Feng Sun

Published in: International Journal of Colorectal Disease | Issue 2/2012

Abstract

Background and aims

To date, the association between special AT-rich sequence-binding protein 1 (SATB1) and colorectal cancer (CRC) has not been reported. This study was aimed at investigating the expression and potential role of SATB1 in human rectal cancers.

Methods

Ninety-three paired samples of rectal cancer and distant normal rectal tissue were analyzed by quantitative real-time PCR (qRT-PCR) and immunohistochemistry (IHC), and the correlations between SATB1 expression and clinicopathological parameters were evaluated. The expression profiles of SATB1 were also investigated in a panel of five human colon carcinoma cell lines.

Results

The general level of SATB1 mRNA in rectal cancer tissues was statistically significantly higher than that in normal mucosa (P = 0.043). The rate of positive SATB1 protein expression in rectal cancers (44.1%) was significantly higher than that in normal tissues (25.8%) by IHC analysis (P = 0.009). Overexpression of SATB1 mRNA was more predominant in patients with earlier onset of rectal cancer (P = 0.033). SATB1 expression correlated with invasive depth and tumor node metastasis (TNM) stage at both protein and mRNA levels (P < 0.05). Furthermore, SATB1 expression in the poorly metastatic KM12C cells was significantly lower than the highly metastatic KM12SM and KM12L4A cells and higher than the HCT116 and SW480 cells (P = 0.001). These results were further confirmed by Western blotting.

Conclusion

Our results indicate that SATB1 may play an important role in the progression of human rectal cancer, which represents a possible new mechanism underlying CRC.

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Title: Correlation of SATB1 overexpression with the progression of human rectal cancer
Authors: Wen-Jian Meng
Hui Yan
Bin Zhou
Wei Zhang
Xiang-Heng Kong
Rong Wang
Lan Zhan
Yuan Li
Zong-Guang Zhou
Xiao-Feng Sun
Publication date: 01-02-2012
Publisher: Springer-Verlag
Published in: International Journal of Colorectal Disease / Issue 2/2012
Print ISSN: 0179-1958
Electronic ISSN: 1432-1262
DOI: https://doi.org/10.1007/s00384-011-1302-9

At a glance: The ONWARDS insulin icodec trials

Springer Medicine

Correlation of SATB1 overexpression with the progression of human rectal cancer

Abstract

Background and aims

Methods

Results

Conclusion

At a glance: The ONWARDS insulin icodec trials

Springer Medicine

Abstract

Background and aims

Methods

Results

Conclusion

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