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Cardiovascular Diabetology
Published in: Cardiovascular Diabetology 1/2011

Open Access 01-12-2011 | Original investigation

Relation between the frequency of CD34+ bone marrow derived circulating progenitor cells and the number of diseased coronary arteries in patients with myocardial ischemia and diabetes

Authors: Ilkay Bozdag-Turan, R Goekmen Turan, C Hakan Turan, Sophie Ludovicy, Ibrahim Akin, Stephan Kische, Nicole S Arsoy, Henrik Schneider, Jasmin Ortak, Tim Rehders, Tina Hermann, Liliya Paranskaya, Peter Kohlschein, Manuela Bastian, A Tulga Ulus, Kurtulus Sahin, Hueseyin Ince, Christoph A Nienaber

Published in: Cardiovascular Diabetology | Issue 1/2011

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Abstract

Background

Bone marrow-derived circulating progenitor cells (BM-CPCs) in patients with coronary heart disease are impaired with respect to number and mobilization. However, it is unknown whether the mobilization of BM-CPCs depends on the number of diseased coronary arteries. Therefore, in our study, we analysed the correlation between the diseased coronary arteries and the frequency of CD34/45+ BM-CPCs in peripheral blood (PB) in patients with ischemic heart disease (IHD).

Methods

The frequency of CD34/45+ BM-CPCs was measured by flow cytometry in 120 patients with coronary 1 vessel (IHD1, n = 40), coronary 2 vessel (IHD2, n = 40), coronary 3 vessel disease (IHD3, n = 40) and in a control group of healthy subjects (n = 40). There was no significant difference of the total number of cardiovascular risk factors between IHD groups, beside diabetes mellitus (DM), which was significantly higher in IHD3 group compared to IHD2 and IHD1 groups.

Results

The frequency of CD34/45+ BM-CPCs was significantly reduced in patients with IHD compared to the control group (CD34/45+; p < 0.001). The frequency of BM-CPCs was impaired in patients with IHD3 compared to IHD1 (CD34/45+; p < 0.001) and to IHD2 (CD34/45+; p = 0.001). But there was no significant difference in frequency of BM-CPCs between the patients with IHD2 and IHD1 (CD34/45+; p = 0.28). In a subgroup we observed a significant negative correlation between levels of hemoglobin AIc (HbAIc) and the frequency of BM-CPCs (CD34/45+; p < 0.001, r = -0.8).

Conclusions

The frequency of CD34/45+ BM-CPCs in PB is impaired in patients with IHD. This impairment may augment with an increased number of diseased coronary arteries. Moreover, the frequency of CD34/45+ BM-CPCs in ischemic tissue is further impaired by diabetes in patients with IHD.
Appendix
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Metadata
Title
Relation between the frequency of CD34+ bone marrow derived circulating progenitor cells and the number of diseased coronary arteries in patients with myocardial ischemia and diabetes
Authors
Ilkay Bozdag-Turan
R Goekmen Turan
C Hakan Turan
Sophie Ludovicy
Ibrahim Akin
Stephan Kische
Nicole S Arsoy
Henrik Schneider
Jasmin Ortak
Tim Rehders
Tina Hermann
Liliya Paranskaya
Peter Kohlschein
Manuela Bastian
A Tulga Ulus
Kurtulus Sahin
Hueseyin Ince
Christoph A Nienaber
Publication date
01-12-2011
Publisher
BioMed Central
Published in
Cardiovascular Diabetology / Issue 1/2011
Electronic ISSN: 1475-2840
DOI
https://doi.org/10.1186/1475-2840-10-107

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