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BMC Complementary Medicine and Therapies

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Open Access 01-12-2014 | Research article

Differences in antioxidant activities of outdoor- and indoor-cultivated Agaricus brasiliensis, and protective effects against carbon tetrachloride-induced acute hepatic injury in mice

Authors: Daisuke Yamanaka, Masuro Motoi, Akitomo Motoi, Naohito Ohno

Published in: BMC Complementary Medicine and Therapies | Issue 1/2014

Abstract

Background

Agaricus brasiliensis (A. brasiliensis) is a medicinal mushroom that exerts various pharmacological actions. We previously demonstrated that different cultivation conditions altered the activity of the polyphenol-related enzymes from this mushroom. However, the influence of cultivation conditions on the antioxidant activity of the fruiting bodies remains unclear. Therefore, in this study we compared the antioxidative effects of fruiting bodies of A. brasiliensis cultivated outdoors and indoors. In addition, we assessed whether different cultivation methods affected the hepatoprotective effects against CCl₄-induced liver injury.

Methods

We assessed the antioxidative effects of mushrooms cultivated in open-air or indoors using the DPPH radical-scavenging assay. Furthermore, we prepared experimental feeds containing outdoor- or indoor-cultivated A. brasiliensis. Acute liver injury was induced by CCl₄ injection in mice that consumed feed containing outdoor- or indoor-cultivated A. brasiliensis. The hepatoprotective effects of these mushrooms were then evaluated by monitoring the reduction in the circulating levels of alanine aminotransferase, aspartate aminotransferase, and lactate dehydrogenase. The significance of the differences between the means was assessed using Student’s t-test. Finally, histopathological analysis of liver was performed.

Results

In the DPPH assay, the antioxidant activity of outdoor-cultivated A. brasiliensis was higher than that of indoor-cultivated mushroom. Moreover, in the mouse model of CCl₄-induced hepatitis, the oral administration of outdoor-cultivated A. brasiliensis reduced liver damage significantly, but indoor-cultivated mushrooms failed to inhibit hepatitis. The hepatoprotective effects of outdoor-cultivated A. brasiliensis were observed even when ingestion commenced only 1 day before CCl₄ injection, and these effects were not affected by excessive heat treatment.

Conclusions

Outdoor cultivation significantly enhanced the antioxidative activity of A. brasiliensis fruiting bodies. In addition, outdoor-cultivated A. brasiliensis was more effective at protecting against CCl₄-induced liver injury in mice than mushrooms grown in a greenhouse.

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Wasser SP, Didukh MY, de A Amazonas MLA, Nevo E, Stamets P, da Eira AF: Is a widely cultivated culinary-medicinal royal Sun Agaricus (the himematsutake mushroom) indeed Agaricus blazei murrill?. Int J Med Mushrooms. 2002, 4 (4): 267-290.

Kerrigan RW: Agaricus subrufescens, a cultivated edible and medicinal mushroom, and its synonyms. Mycologia. 2005, 97 (1): 12-24. 10.3852/mycologia.97.1.12.CrossRefPubMed

Wisitrassameewong K, Karunarathna SC, Thongklang N, Zhao R, Callac P, Moukha S, Ferandon C, Chukeatirote E, Hyde KD: Agaricus subrufescens: a review. Saudi J Biol Sci. 2012, 19 (2): 131-146. 10.1016/j.sjbs.2012.01.003.CrossRefPubMedPubMedCentral

Bouike G, Nishitani Y, Shiomi H, Yoshida M, Azuma T, Hashimoto T, Kanazawa K, Mizuno M: Oral treatment with extract of Agaricus blazei murill enhanced Th1 response through intestinal epithelial cells and suppressed OVA-sensitized allergy in mice. Evid Based Complement Alternat Med. 2011, 2011: 532180-CrossRefPubMed

Ellertsen LK, Hetland G: An extract of the medicinal mushroom Agaricus blazei Murill can protect against allergy. Clin Mol Allergy. 2009, 7: 6-10.1186/1476-7961-7-6.CrossRefPubMedPubMedCentral

Liu Y, Fukuwatari Y, Okumura K, Takeda K, Ishibashi KI, Furukawa M, Ohno N, Mori K, Gao M, Motoi M: Immunomodulating activity of Agaricus brasiliensis KA21 in mice and in human volunteers. Evid Based Complement Alternat Med. 2008, 5 (2): 205-219. 10.1093/ecam/nem016.CrossRefPubMed

Ohno N, Akanuma AM, Miura NN, Adachi Y, Motoi M: (1-->3)-beta-D-glucan in the fruit bodies of Agaricus blazei. Pharm Pharmacol Lett. 2001, 11 (2): 87-90.

Ohno N, Furukawa M, Miura NN, Adachi Y, Motoi M, Yadomae T: Antitumor beta glucan from the cultured fruit body of Agaricus blazei. Biol Pharm Bull. 2001, 24 (7): 820-828. 10.1248/bpb.24.820.CrossRefPubMed

Yamanaka D, Motoi M, Ishibashi K, Miura NN, Adachi Y, Ohno N: Effect of Agaricus brasiliensis-derived cold water extract on Toll-like receptor 2-dependent cytokine production in vitro. Immunopharmacol Immunotoxicol. 2012, 34 (4): 561-570. 10.3109/08923973.2011.633526.CrossRefPubMed

10.

Kasai H, He LM, Kawamura M, Yang PT, Deng XW, Munkanta M, Yamashita A, Terunuma H, Hirama M, Horiuchi I, Natori T, Koga T, Amano Y, Yamaguchi N, Ito M: IL-12 Production induced by Agaricus blazei fraction H (ABH) involves toll-like receptor (TLR). Evid Based Complement Alternat Med. 2004, 1 (3): 259-267. 10.1093/ecam/neh043.CrossRefPubMedPubMedCentral

11.

Yamanaka D, Tada R, Adachi Y, Ishibashi K, Motoi M, Iwakura Y, Ohno N: Agaricus brasiliensis-derived beta-glucans exert immunoenhancing effects via a dectin-1-dependent pathway. Int Immunopharmacol. 2012, 14 (3): 311-319. 10.1016/j.intimp.2012.07.017.CrossRefPubMed

12.

Chang JB, Wu MF, Yang YY, Leu SJ, Chen YL, Yu CS, Yu CC, Chang SJ, Lu HF, Chung JG: Carbon tetrachloride-induced hepatotoxicity and its amelioration by Agaricus blazei Murrill extract in a mouse model. In Vivo. 2011, 25 (6): 971-976.PubMed

13.

Al-Dbass AM, Al-Daihan SK, Bhat RS: Agaricus blazei Murill as an efficient hepatoprotective and antioxidant agent against CCl₄-induced liver injury in rats. Saudi J Biol Sci. 2012, 19 (3): 303-309. 10.1016/j.sjbs.2012.03.004.CrossRefPubMedPubMedCentral

14.

Yuan LP, Chen FH, Ling L, Dou PF, Bo H, Zhong MM, Xia LJ: Protective effects of total flavonoids of Bidens pilosa L. (TFB) on animal liver injury and liver fibrosis. J Ethnopharmacol. 2008, 116 (3): 539-546. 10.1016/j.jep.2008.01.010.CrossRefPubMed

15.

Hashimoto S, Akanuma AM, Motoi M, Imai N, Rodrignes CA, Nameda S, Miura NN, Adachi Y, Ohno N: Effect of culture conditions on the chemical composition and biological activities of Agaricus brasiliensis S. Wasser et al. (Agaricomycetideae). Int J Med Mushrooms. 2006, 8 (4): 329-341. 10.1615/IntJMedMushr.v8.i4.40.CrossRef

16.

Blois MS: Antioxidant determinations by the use of a stable free radical. Nature. 1958, 181 (4617): 1199-1200. 10.1038/1811199a0.CrossRef

17.

Mourao F, Harue Umeo S, Seiko Takemura O, Andrea Linde G, Barros Colauto N: Antioxidant Activity of Agaricus brasiliensis Basidiocarps on Different Maturation Phases. Braz J Microbiol. 2011, 42 (1): 197-202. 10.1590/S1517-83822011000100024.CrossRefPubMedPubMedCentral

18.

Hsu CH, Hwang KC, Chiang YH, Chou P: The mushroom Agaricus blazei Murill extract normalizes liver function in patients with chronic hepatitis B. J Altern Complement Med. 2008, 14 (3): 299-301. 10.1089/acm.2006.6344.CrossRefPubMed

19.

Wu MF, Hsu YM, Tang MC, Chen HC, Chung JG, Lu HF, Lin JP, Tang NY, Yeh C, Yeh MY: Agaricus blazei Murill extract abrogates CCl₄-induced liver injury in rats. In Vivo. 2011, 25 (1): 35-40.PubMed

20.

Zhang C, Han C, Zhao B, Yu H: The protective effects of aqueous extracts of wild-growing and fermented Royal Sun mushroom, Agaricus brasiliensis S. Wasser et al. (higher basidiomycetes), in CCl4-induced oxidative damage in rats. Int J Med Mushrooms. 2012, 14 (6): 557-561. 10.1615/IntJMedMushr.v14.i6.30.CrossRefPubMed

21.

Mukai H, Watanabe T, Ando M, Katsumata N: An alternative medicine, Agaricus blazei, may have induced severe hepatic dysfunction in cancer patients. Jpn J Clin Oncol. 2006, 36 (12): 808-810. 10.1093/jjco/hyl108.CrossRefPubMed

22.

Holick MF: Sunlight and vitamin D for bone health and prevention of autoimmune diseases, cancers, and cardiovascular disease. Am J Clin Nutr. 2004, 80 (6 Suppl): 1678S-1688S.PubMed

23.

Martineau AR, Wilkinson RJ, Wilkinson KA, Newton SM, Kampmann B, Hall BM, Packe GE, Davidson RN, Eldridge SM, Maunsell ZJ, Rainbow SJ, Berry JL, Griffiths CJ: A single dose of vitamin D enhances immunity to mycobacteria. Am J Respir Crit Care Med. 2007, 176 (2): 208-213. 10.1164/rccm.200701-007OC.CrossRefPubMed

24.

Saad EA: Curative and protective effects of L-arginine on carbon tetrachloride-induced hepatotoxicity in mice. Biochem Biophys Res Commun. 2012, 423 (1): 147-151. 10.1016/j.bbrc.2012.05.102.CrossRefPubMed

25.

Jiang W, Gao M, Sun S, Bi A, Xin Y, Han X, Wang L, Yin Z, Luo L: Protective effect of L-theanine on carbon tetrachloride-induced acute liver injury in mice. Biochem Biophys Res Commun. 2012, 422 (2): 344-350. 10.1016/j.bbrc.2012.05.022.CrossRefPubMed

26.

Bai X, Qiu A, Guan J, Shi Z: Antioxidant and protective effect of an oleanolic acid-enriched extract of A. deliciosa root on carbon tetrachloride induced rat liver injury. Asia Pac J Clin Nutr. 2007, 16 (Suppl 1): 169-173.PubMed

27.

Xia D, Fan Y, Zhang P, Fu Y, Ju M, Zhang X: Protective effects of the flavonoid-rich fraction from rhizomes of Smilax glabra Roxb. on carbon tetrachloride-induced hepatotoxicity in rats. J Membr Biol. 2013, 246 (6): 479-485. 10.1007/s00232-013-9560-9.CrossRefPubMed

The pre-publication history for this paper can be accessed here:http://www.biomedcentral.com/1472-6882/14/454/prepub

Title: Differences in antioxidant activities of outdoor- and indoor-cultivated Agaricus brasiliensis, and protective effects against carbon tetrachloride-induced acute hepatic injury in mice
Authors: Daisuke Yamanaka
Masuro Motoi
Akitomo Motoi
Naohito Ohno
Publication date: 01-12-2014
Publisher: BioMed Central
Published in: BMC Complementary Medicine and Therapies / Issue 1/2014
Electronic ISSN: 2662-7671
DOI: https://doi.org/10.1186/1472-6882-14-454

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Differences in antioxidant activities of outdoor- and indoor-cultivated Agaricus brasiliensis, and protective effects against carbon tetrachloride-induced acute hepatic injury in mice

Abstract

Background

Methods

Results

Conclusions

Keynote webinar | Spotlight on sleep in brain health

Springer Medicine

Abstract

Background

Methods

Results

Conclusions

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