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Open Access 01-12-2013 | Research article

TGFβ signaling regulates lipogenesis in human sebaceous glands cells

Authors: Adrian J McNairn, Yanne Doucet, Julien Demaude, Marion Brusadelli, Christopher B Gordon, Armando Uribe-Rivera, Paul F Lambert, Charbel Bouez, Lionel Breton, Géraldine Guasch

Published in: BMC Dermatology | Issue 1/2013

Abstract

Background

Sebaceous glands are components of the skin essential for its normal lubrication by the production of sebum. This contributes to skin health and more importantly is crucial for the skin barrier function. A mechanistic understanding of sebaceous gland cells growth and differentiation has lagged behind that for keratinocytes, partly because of a lack of an in vitro model that can be used for experimental manipulation.

Methods

We have developed an in vitro culture model to isolate and grow primary human sebocytes without transformation that display functional characteristics of sebocytes. We used this novel method to probe the effect of Transforming Growth Factor β (TGFβ) signaling on sebocyte differentiation, by examining the expression of genes involved in lipogenesis upon treatment with TGFβ1. We also repressed TGFβ signaling through knockdown of the TGFβ Receptor II to address if the effect of TGFβ activation is mediated via canonical Smad signal transduction.

Results

We find that activation of the TGFβ signaling pathway is necessary and sufficient for maintaining sebocytes in an undifferentiated state. The presence of TGFβ ligand triggered decreased expression in genes required for the production of characteristics sebaceous lipids and for sebocyte differentiation such as FADS2 and PPARγ, thereby decreasing lipid accumulation through the TGFβ RII-Smad2 dependent pathway.

Conclusion

TGFβ signaling plays an essential role in sebaceous gland regulation by maintaining sebocytes in an undifferentiated state. This data was generated using a novel method for human sebocyte culture, which is likely to prove generally useful in investigations of sebaceous gland growth and differentiation. These findings open a new paradigm in human skin biology with important implications for skin therapies.

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The pre-publication history for this paper can be accessed here:http://www.biomedcentral.com/1471-5945/13/2/prepub

Title: TGFβ signaling regulates lipogenesis in human sebaceous glands cells
Authors: Adrian J McNairn
Yanne Doucet
Julien Demaude
Marion Brusadelli
Christopher B Gordon
Armando Uribe-Rivera
Paul F Lambert
Charbel Bouez
Lionel Breton
Géraldine Guasch
Publication date: 01-12-2013
Publisher: BioMed Central
Published in: BMC Dermatology / Issue 1/2013
Electronic ISSN: 1471-5945
DOI: https://doi.org/10.1186/1471-5945-13-2

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TGFβ signaling regulates lipogenesis in human sebaceous glands cells

Abstract

Background

Methods

Results

Conclusion

Keynote webinar | Spotlight on sleep in brain health

Springer Medicine

Abstract

Background

Methods

Results

Conclusion

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