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Open Access 01-12-2010 | Research article

Platelet autologous growth factors decrease the osteochondral regeneration capability of a collagen-hydroxyapatite scaffold in a sheep model

Authors: Elizaveta Kon, Giuseppe Filardo, Marco Delcogliano, Milena Fini, Francesca Salamanna, Gianluca Giavaresi, Ivan Martin, Maurilio Marcacci

Published in: BMC Musculoskeletal Disorders | Issue 1/2010

Abstract

Background

Current research aims to develop innovative approaches to improve chondral and osteochondral regeneration. The objective of this study was to investigate the regenerative potential of platelet-rich plasma (PRP) to enhance the repair process of a collagen-hydroxyapatite scaffold in osteochondral defects in a sheep model.

Methods

PRP was added to a new, multi-layer gradient, nanocomposite scaffold that was obtained by nucleating collagen fibrils with hydroxyapatite nanoparticles. Twenty-four osteochondral lesions were created in sheep femoral condyles. The animals were randomised to three treatment groups: scaffold, scaffold loaded with autologous PRP, and empty defect (control). The animals were sacrificed and evaluated six months after surgery.

Results

Gross evaluation and histology of the specimens showed good integration of the chondral surface in both treatment groups. Significantly better bone regeneration and cartilage surface reconstruction were observed in the group treated with the scaffold alone. Incomplete bone regeneration and irregular cartilage surface integration were observed in the group treated with the scaffold where PRP was added. In the control group, no bone and cartilage defect healing occurred; defects were filled with fibrous tissue. Quantitative macroscopic and histological score evaluations confirmed the qualitative trends observed.

Conclusions

The hydroxyapatite-collagen scaffold enhanced osteochondral lesion repair, but the combination with platelet growth factors did not have an additive effect; on the contrary, PRP administration had a negative effect on the results obtained by disturbing the regenerative process. In the scaffold + PRP group, highly amorphous cartilaginous repair tissue and poorly spatially organised underlying bone tissue were found.

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The pre-publication history for this paper can be accessed here:http://www.biomedcentral.com/1471-2474/11/220/prepub

Title: Platelet autologous growth factors decrease the osteochondral regeneration capability of a collagen-hydroxyapatite scaffold in a sheep model
Authors: Elizaveta Kon
Giuseppe Filardo
Marco Delcogliano
Milena Fini
Francesca Salamanna
Gianluca Giavaresi
Ivan Martin
Maurilio Marcacci
Publication date: 01-12-2010
Publisher: BioMed Central
Published in: BMC Musculoskeletal Disorders / Issue 1/2010
Electronic ISSN: 1471-2474
DOI: https://doi.org/10.1186/1471-2474-11-220

Keynote webinar | Spotlight on medication adherence

Springer Medicine

Platelet autologous growth factors decrease the osteochondral regeneration capability of a collagen-hydroxyapatite scaffold in a sheep model

Abstract

Background

Methods

Results

Conclusions

Keynote webinar | Spotlight on medication adherence

Springer Medicine

Abstract

Background

Methods

Results

Conclusions

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