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BMC Musculoskeletal Disorders
Published in: BMC Musculoskeletal Disorders 1/2010

Open Access 01-12-2010 | Research article

Analysis of human synovial and bone marrow mesenchymal stem cells in relation to heat-inactivation of autologous and fetal bovine serums

Authors: Akimoto Nimura, Takeshi Muneta, Koji Otabe, Hideyuki Koga, Young-Jin Ju, Tomoyuki Mochizuki, Koji Suzuki, Ichiro Sekiya

Published in: BMC Musculoskeletal Disorders | Issue 1/2010

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Abstract

Background

Though sera are essential for Mesenchymal stem cells (MSCs), the effect of heat-inactivation remains unknown. Autologous human serum is recommended for clinical use; however, it is unclear whether differentiation potentials are maintained. To examine whether heat-inactivation of serum affected the proliferation and whether autologous human serum influenced their multipotentiality.

Methods

After whole blood collection, human synovium and bone marrow were harvested. Nucleated cells were expanded with autologous human serum and FBS.

Results

Heat-inactivation of autologous human serum enhanced proliferation of synovial MSCs. Heat-inactivation of each types of serum didn't affect calcification of synovial MSCs. The induction of calcification increased ALP activity, with the exception of bone marrow MSCs with autologous human serum. For adipogenesis of synovial MSCs, the Oil Red-O positive colony forming efficiency with autologous human serum was similar to or less than that with FBS.

Conclusion

These clarified the processing of human autologous serum and the influence of different sera for differentiation of synovial and bone marrow MSCs.
Appendix
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Metadata
Title
Analysis of human synovial and bone marrow mesenchymal stem cells in relation to heat-inactivation of autologous and fetal bovine serums
Authors
Akimoto Nimura
Takeshi Muneta
Koji Otabe
Hideyuki Koga
Young-Jin Ju
Tomoyuki Mochizuki
Koji Suzuki
Ichiro Sekiya
Publication date
01-12-2010
Publisher
BioMed Central
Published in
BMC Musculoskeletal Disorders / Issue 1/2010
Electronic ISSN: 1471-2474
DOI
https://doi.org/10.1186/1471-2474-11-208

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