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BMC Public Health
Published in: BMC Public Health 1/2012

Open Access 01-12-2012 | Research article

Air sampling procedures to evaluate microbial contamination: a comparison between active and passive methods in operating theatres

Authors: Christian Napoli, Vincenzo Marcotrigiano, Maria Teresa Montagna

Published in: BMC Public Health | Issue 1/2012

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Abstract

Background

Since air can play a central role as a reservoir for microorganisms, in controlled environments such as operating theatres regular microbial monitoring is useful to measure air quality and identify critical situations. The aim of this study is to assess microbial contamination levels in operating theatres using both an active and a passive sampling method and then to assess if there is a correlation between the results of the two different sampling methods.

Methods

The study was performed in 32 turbulent air flow operating theatres of a University Hospital in Southern Italy. Active sampling was carried out using the Surface Air System and passive sampling with settle plates, in accordance with ISO 14698. The Total Viable Count (TVC) was evaluated at rest (in the morning before the beginning of surgical activity) and in operational (during surgery).

Results

The mean TVC at rest was 12.4 CFU/m3 and 722.5 CFU/m2/h for active and passive samplings respectively. The mean in operational TVC was 93.8 CFU/m3 (SD = 52.69; range = 22-256) and 10496.5 CFU/m2/h (SD = 7460.5; range = 1415.5-25479.7) for active and passive samplings respectively. Statistical analysis confirmed that the two methods correlate in a comparable way with the quality of air.

Conclusion

It is possible to conclude that both methods can be used for general monitoring of air contamination, such as routine surveillance programs. However, the choice must be made between one or the other to obtain specific information.
Appendix
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Literature
1.
Sehulster L, Chinn RY: Guidelines for environmental infection control in health-care facilities. Recommendations of CDC and the Healthcare Infection Control Practices Advisory Committee (HICPAC). MMWR Recomm Rep. 2003, 52: 1-42.PubMed
2.
Napoli C, Fasano F, Iatta R, Barbuti G, Cuna C, Montagna MT: Legionella spp. and legionellosis in southeastern Italy: disease epidemiology and environmental surveillance in community and health care facilities. BMC Public Health. 2010, 10: 660-10.1186/1471-2458-10-660.CrossRefPubMedPubMedCentral
3.
Pasquarella C, Veronesi L, Castiglia P, Liguori G, Montagna MT, Napoli C, Rizzetto R, Torre I, Masia MD, Di Onofrio V, Colucci ME, Tinteri C, Tanzi M: Italian multicentre study on microbial environmental contamination in dental clinics: a pilot study. Sc Total Environ. 2010, 408: 4045-4051. 10.1016/j.scitotenv.2010.05.010.CrossRef
4.
Tseng CC, Chang LY, CS L: Detection of airborne viruses in a pediatrics department measured using real-time qPCR coupled to an air-sampling filter method. J. Environ. Health. 2010, 73: 22-28.PubMed
5.
Vescia N, Brenier-Pinchart MP, Osborn JF, Cerquetani F, Cavarischia R, Grillot R, D’Alessandro D: Field validation of a dusting cloth for mycological surveillance of surfaces. Am. J. Infect. Control. 2011, 39: 156-158. 10.1016/j.ajic.2010.05.018.CrossRefPubMed
6.
Demir F: A survey on prevention of surgical infections in operating theaters. Worldviews Evid. Based Nurs. 2009, 6: 102-113. 10.1111/j.1741-6787.2009.00152.x.CrossRefPubMed
7.
Beldi G, Bisch-Knaden S, Banz V, Mühlemann K, Candinas D: Impact of intraoperative behavior on surgical site infections. Am J Surg. 2009, 198: 157-162. 10.1016/j.amjsurg.2008.09.023.CrossRefPubMed
8.
Weiss KD, Osborne SF, Callahan-Lyon P: Prevention of surgical-site infections. N. Engl. J. Med. 2010, 362: 1541-1542.PubMed
9.
Barrow C: A patient’s journey through the operating department from an infection control perspective. J. Perioper. Pract. 2009, 19: 94-98.PubMed
10.
Buettcher M, Heininger U: Prospective surveillance of nosocomial viral infections during and after hospitalization at a university children’s hospital. Pediatr. Infect. Dis. J. 2010, 29: 950-956. 10.1097/INF.0b013e3181e32d97.CrossRefPubMed
11.
Charnley J: Postoperative infection after total hip replacement with special reference to air contamination in the operating room. Clin. Orthop. 1972, 87: 167-187. 10.1097/00003086-197209000-00020.CrossRefPubMed
12.
Hansis ML: Hygiene between tradition and implementation. Orthopade. 2004, 33: 412-415. 10.1007/s00132-003-0625-9.CrossRefPubMed
13.
Pasquarella C, Sansebastiano GE, Ferretti S, Saccani E, Fanti M, Moscato U, Giannetti G, Fornia S, Cortellini P, Vitali P, Signorelli C: A mobile laminar air flow unit to reduce air bacterial contamination at surgical area in a conventionally ventilated operating theatre. J Josp Infect. 2007, 66: 313-319.CrossRef
14.
Dharan S, Pittet D: Environmental controls in operating theatres. J. Hosp. Infect. 2002, 51: 79-84. 10.1053/jhin.2002.1217.CrossRefPubMed
15.
Scaltriti S, Cencetti S, Rovesti S, Marchesi I, Bargellini A, Borella P: Risk factors for particulate and microbial contamination of air in operating theatres. J. Hosp. Infect. 2007, 66: 320-326. 10.1016/j.jhin.2007.05.019.CrossRefPubMed
16.
Gosden PE, MacGowan AP, Bannister GC: Importance of air quality and related factors in the prevention of infection in orthopaedic implant surgery. J. Hosp. Infect. 1998, 39: 173-180. 10.1016/S0195-6701(98)90255-9.CrossRefPubMed
17.
Oren I, Haddad N, Finkelstein R, Rowe JM: Invasive pulmonary aspergillosis in neutropenic patients during hospital construction: before and after chemoprophylaxis and institution of HEPA filters. Am. J. Hematol. 2001, 66: 257-262. 10.1002/ajh.1054.CrossRefPubMed
18.
Pasquarella C, Albertini R, Dall’aglio P, Saccani E, Sansebastiano GE, Signorelli C: Air microbial sampling: the state of the art. Ig. Sanita Pubbl. 2008, 64: 79-120.PubMed
19.
Cleanrooms and associated controlled environments – Biocontamination control. Part 1: General principles and methods. 2003, UNI, Milano, 14698–1
20.
Blomquist G: Sampling of biological particles. Analyst. 1994, 119: 53-56.PubMed
21.
22.
Pasquarella C, Pitzurra O, Savino A: The index of microbial air contamination. J. Hosp. Infect. 2000, 46: 241-256. 10.1053/jhin.2000.0820.CrossRefPubMed
23.
French MLV, Eitzen HE, Ritter MA, Leland DS: Environmental control of microbial contamination in the operating room. Wound Healing and Wound Infection. Edited by: Hunt TK. 1980, Appleton-Century Crofis, New York, 254-261.
24.
Whyte W: Sterility assurance and models for assessing airborne bacterial contamination. J. Parenter. Sci. Technol. 1986, 40: 188-197.PubMed
25.
Orpianesi C, Cresci A, La Rosa F, Saltalamacchia G, Tarsi R: Evaluation of microbial contamination in a hospital environment. Comparison between the Surface Air System and the traditional method. Nuovi Ann Ig Microbiol. 1983, 34: 171-185.PubMed
26.
Perdelli F, Sartini M, Orlando M, Secchi V, Cristina ML: Relationship between settling microbial load and suspended microbial load in operating rooms. Ann. Ig. 2000, 12: 373-380.PubMed
27.
Petti S, Iannazzo S, Tarsitani G: Comparison between different methods to monitor the microbial level of indoor air contamination in the dental office. Ann. Ig. 2003, 15: 725-733.PubMed
28.
Sayer WJ, MacKnight NM, Wilson HW: Hospital airborne bacteria as estimated by the Andersen sampler versus the gravity settling culture plate. Am. J. Clin. Pathol. 1972, 58: 558-566.CrossRefPubMed
29.
Anhang 4 zur KlatAS. 2007, IMA-Methode, Beschreibung der
30.
31.
De Hoog GS, Guarro J, Gené J, Figueras MJ: Atlas of Clinical Fungi. 2009, Centraalbureau voor Schimmelcultures, Utrecht, 3
32.
Napoli C, Tafuri S, Montenegro L, Cassano M, Notarnicola A, Lattarulo S, Montagna MT, Moretti B: Air sampling methods to evaluate microbial contamination in operating theatres: results of a comparative study in an orthopaedics department. J. Hosp. Infect. 2012, 80: 128-132. 10.1016/j.jhin.2011.10.011.CrossRefPubMed
33.
Friberg B, Friberg S, Burman LG: Correlation between surface and air counts of particles carrying aerobic bacteria in operating rooms with turbulent ventilation: an experimental study. J. Hosp. Infect. 1999, 42: 61-68. 10.1053/jhin.1998.0542.CrossRefPubMed
34.
Moretti B, Larocca A, Napoli C, Martinelli D, Paolillo L, Cassano M, Notarnicola A, Moretti L, Pesce V: Active warming systems to maintain perioperative normothermia in hip replacement surgery: A therapeutic aid or a vector of infection?. J. Hosp. Infect. 2009, 73: 58-63. 10.1016/j.jhin.2009.06.006.CrossRefPubMed
35.
Montagna MT, De Giglio O, Napoli C, Lovero G, Caggiano G, Delia M, Pastore D, Santoro N, Specchia G: Invasive Fungal Infections in Patients with Hematologic Malignancies (Aurora Project): Lights and Shadows During 18-Months Surveillance. Int. J. Mol. Sci. 2012, 13: 774-787. 10.3390/ijms13010774.CrossRefPubMedPubMedCentral
36.
Verhoeff AP, van Wijnen JH, Boleij JS, Brunekreef B, van Reenen-Hoekstra ES, Samson RA: Enumeration and identification of airborne viable mould propagules in houses. A field comparison of selected techniques. Allergy. 1990, 45: 275-284. 10.1111/j.1398-9995.1990.tb00496.x.CrossRefPubMed
37.
Asefa DT, Gjerde RO, Sidhu MS, Langsrud S, Kure CF, Nesbakken T, Skaar I: The performance of SAS-super-180 air sampler and settle plates for assessing viable fungal particles in the air of dry-cured meat production facility. Food Control. 2009, 20: 997-1001. 10.1016/j.foodcont.2008.11.011.CrossRef
38.
Alberti C, Bouakline A, Ribaud P, Lacroix C, Rousselot P, Leblanc T, Derouin F: Relationship between environmental fungal contamination and the incidence of invasive aspergillosis in haematology patients. J. Hosp. Infect. 2001, 48: 198-206. 10.1053/jhin.2001.0998.CrossRefPubMed
39.
Crimi P, Valgiusti M, Macrina G, Grieco A, Massone L, Ciucci A, Ansaldi F, Sticchi L, Sasso L, del Buono S, Durando P: Evaluation of microbial contamination of air in two haematology departments equipped with ventilation systems with different filtration devices. J. Prev. Med. Hyg. 2009, 50: 33-36.PubMed
40.
Kundsin RB: Architectural Design and Indoor Microbial Pollution. The microbiologist’s role in evaluating the hygienic environment. 1988, Oxford University Press, New York, 103-122.
41.
Whyte W: In support of settle plates. PDA J Pharm Scien Technol. 1996, 50: 201-204.
Metadata
Title
Air sampling procedures to evaluate microbial contamination: a comparison between active and passive methods in operating theatres
Authors
Christian Napoli
Vincenzo Marcotrigiano
Maria Teresa Montagna
Publication date
01-12-2012
Publisher
BioMed Central
Published in
BMC Public Health / Issue 1/2012
Electronic ISSN: 1471-2458
DOI
https://doi.org/10.1186/1471-2458-12-594

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