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BMC Ophthalmology

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Open Access 01-12-2015 | Research article

Square-edge intraocular lenses and epithelial lens cell proliferation: implications on posterior capsule opacification in an in vitro model

Authors: Rita Mencucci, Eleonora Favuzza, Carlotta Boccalini, Jean-Jacques Gicquel, Laura Raimondi

Published in: BMC Ophthalmology | Issue 1/2015

Abstract

Background

To evaluate lens epithelial cell (LEC) proliferation with two different designs (one-piece or three-piece) of hydrophobic acrylic IOLs with 360° square optic edge using an in vitro culture model of posterior capsule opacification (PCO).

Methods

This experimental study was conducted at the Department of NEUROFARBA, Section of Pharmacology, University of Florence, Italy. Human LECs were seeded and cultured in transwell cell culture inserts coated with a type-IV collagen membrane on which an IOL (one-piece Tecnis-1 or three-piece AR40E, Abbott Medical Optics Inc.) had been previously placed. As control, cells were plated on the insert membrane without an IOL. At day six (cells confluent in controls) IOLs were removed and cell counting, viability and cell density under and outside the IOLs were evaluated.

Results

No statistically significant difference in the number of cells (p > 0.05) between inserts with the one-piece and three-piece IOLs was found. Cell density in the area under each IOL was significantly lower than in the area outside of it (p < 0.05), or in the control insert. (p < 0.05). Cell density under the single-piece IOL was not significantly different from that under the three-piece IOL (p > 0.05).

Conclusions

A 360° sharp-edge played a crucial role in avoiding LEC migration under the IOL and preventing the formation of PCO after cataract surgery. Long term clinical evaluation is necessary to estimate functional results.

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The pre-publication history for this paper can be accessed here:http://www.biomedcentral.com/1471-2415/15/5/prepub

Title: Square-edge intraocular lenses and epithelial lens cell proliferation: implications on posterior capsule opacification in an in vitro model
Authors: Rita Mencucci
Eleonora Favuzza
Carlotta Boccalini
Jean-Jacques Gicquel
Laura Raimondi
Publication date: 01-12-2015
Publisher: BioMed Central
Published in: BMC Ophthalmology / Issue 1/2015
Electronic ISSN: 1471-2415
DOI: https://doi.org/10.1186/1471-2415-15-5

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Springer Medicine

Square-edge intraocular lenses and epithelial lens cell proliferation: implications on posterior capsule opacification in an in vitro model

Abstract

Background

Methods

Results

Conclusions

Keynote webinar | Spotlight on medication adherence

Springer Medicine

Abstract

Background

Methods

Results

Conclusions

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