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BMC Cancer
Published in: BMC Cancer 1/2014

Open Access 01-12-2014 | Research article

MicroRNA in exosomes isolated directly from the liver circulation in patients with metastatic uveal melanoma

Authors: Maria Eldh, Roger Olofsson Bagge, Cecilia Lässer, Joar Svanvik, Margareta Sjöstrand, Jan Mattsson, Per Lindnér, Dong-Sic Choi, Yong Song Gho, Jan Lötvall

Published in: BMC Cancer | Issue 1/2014

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Abstract

Background

Uveal melanoma is a tumour arising from melanocytes of the eye, and 30 per cent of these patients develop liver metastases. Exosomes are small RNA containing nano-vesicles released by most cells, including malignant melanoma cells. This clinical translational study included patients undergoing isolated hepatic perfusion (IHP) for metastatic uveal melanoma, from whom exosomes were isolated directly from liver perfusates. The objective was to determine whether exosomes are present in the liver circulation, and to ascertain whether these may originate from melanoma cells.

Methods

Exosomes were isolated from the liver perfusate of twelve patients with liver metastases from uveal melanoma undergoing IHP. Exosomes were visualised by electron microscopy, and characterised by flow cytometry, Western blot and real-time PCR. Furthermore, the concentration of peripheral blood exosomes were measured and compared to healthy controls.

Results

The liver perfusate contained Melan-A positive and RNA containing exosomes, with similar miRNA profiles among patients, but dissimilar miRNA compared to exosomes isolated from tumor cell cultures. Patients with metastatic uveal melanoma had a higher concentration of exosomes in their peripheral venous blood compared to healthy controls.

Conclusions

Melanoma exosomes are released into the liver circulation in metastatic uveal melanoma, and is associated with higher concentrations of exosomes in the systemic circulation. The exosomes isolated directly from liver circulation contain miRNA clusters that are different from exosomes from other cellular sources.
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Metadata
Title
MicroRNA in exosomes isolated directly from the liver circulation in patients with metastatic uveal melanoma
Authors
Maria Eldh
Roger Olofsson Bagge
Cecilia Lässer
Joar Svanvik
Margareta Sjöstrand
Jan Mattsson
Per Lindnér
Dong-Sic Choi
Yong Song Gho
Jan Lötvall
Publication date
01-12-2014
Publisher
BioMed Central
Published in
BMC Cancer / Issue 1/2014
Electronic ISSN: 1471-2407
DOI
https://doi.org/10.1186/1471-2407-14-962

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